Extraction method of cyanidin

An extraction method and anthocyanin technology, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve problems such as consumer insecurity, and achieve the effects of simplifying extraction steps, reducing costs, and improving dissolution rate

Inactive Publication Date: 2015-12-30
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In existing reports, methanol, acetone, hydrochloric acid, etc. can be used to extract anthocyanins, but due to the potential toxicity of residual solvents, the extracts are not safe for consumers

Method used

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  • Extraction method of cyanidin
  • Extraction method of cyanidin
  • Extraction method of cyanidin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. The design ratio of solid to liquid is 1:20, 1:30, 1:40 (m / v, g / ml), the extraction temperature is 40, 50, 60°C, and the extraction time is 40, 60, 80min each time. Orthogonal experiment was designed, and the factor levels are shown in Table 1.

[0029] 2. Take fresh leaves, wash them, dry them, remove the main veins, cut the leaves into pieces, weigh 0.4g, and place them in a mortar. Add a small amount of pre-cooled citric acid-sodium citrate buffer solution with a pH of 3 to the mortar, grind and homogenize the slurry under ice bath conditions, and transfer it to a centrifuge tube.

[0030] Table 1 Orthogonal factor level table

[0031]

[0032] 3. According to the orthogonal test designed in the above step 1, add citric acid-sodium citrate buffer solution to the mortar several times to rinse the mortar, transfer the rinse solution to a centrifuge tube, and mix well so that the final material-to-liquid ratio is respectively 1:20, 1:30, 1:40 (m / v, g / ml), the ce...

Embodiment 2

[0038] 1. Take a total of 15 parts of fresh leaves, wash them, dry them, remove the main veins, cut up the leaves and weigh them, each part is 0.4g, and put them in a mortar.

[0039] 2. Add a small amount of pre-cooled citric acid-sodium citrate buffer solution with a pH of 3 to the mortar, grind and homogenize in an ice bath, transfer the material liquid into a centrifuge tube and number it. Continue to add citric acid-sodium citrate buffer solution to the mortar, rinse the mortar several times, then transfer to the above-mentioned corresponding centrifuge tubes, mix well, and finally, the ratio of solid to liquid is 1:10, 1:20, 1:30, 1:40, 1:50 (m / v, g / ml), each material-liquid ratio was repeated 3 times, and the feed liquid in the centrifuge tube was extracted at 50°C for 60 minutes in the dark and shaken, and at 4°C, 4000rpm / min, centrifuge for 10min. The supernatant was filtered through filter paper and became the sample solution.

[0040] 3. Sample measurement: the sa...

Embodiment 3

[0042] 1. Take a total of 15 parts of fresh leaves, wash them, dry them, remove the main veins, cut up the leaves and weigh them, 0.4g per part, and put them in a mortar.

[0043] 2. Add a small amount of pre-cooled citric acid-sodium citrate buffer solution with a pH of 3 to the mortar, grind and homogenize in an ice bath, then transfer the material liquid into a centrifuge tube and number it. Continue to add citric acid-sodium citrate buffer solution to the mortar respectively, rinse the mortar several times, then transfer to the above-mentioned centrifuge tubes respectively, mix well, and finally, the solid-liquid ratio is 1: 30 (m / v, g / ml), the feed liquid in the centrifuge tube was extracted at 50°C for 20, 40, 60, 80, and 100 min in the dark, respectively, and each extraction time was repeated 3 times. The centrifuge tube was centrifuged at 4°C, 4000 rpm / min for 10 min. The supernatant was filtered through filter paper and became the sample solution.

[0044]3. Sample ...

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Abstract

The invention belongs to the field of plant effective component extraction, and provides an extraction method of cyanidin. The method comprises the following steps: (1) sample preparation; (2) leaf grinding; and (3) cyanidin extraction. The orthogonal test by using a citric acid-sodium citrate buffer solution as an extraction solvent detects that the sequence of factors responding to the purple lettuce cyanidin extraction effect is as follows: material/liquid ratio>extraction temperature>extraction time; and therefore, the material/liquid ratio is preferentially determined in the cyanidin extraction process. The control on the material/liquid ratio, extraction time, extraction temperature and extracting solution pH value proves that the method enhances the extraction rate and cyanidin leaching rate on the premise of lowering the cost and simplifying the process. Since all the extraction solvents are nontoxic and are reagents widely used as food additives, the method can satisfy the requirements of modern people for safety, and thus, has wide applicability in the fields of food, health care, drugs and the like.

Description

technical field [0001] The invention belongs to the field of extraction of effective plant components, and relates to an extraction method of anthocyanins. Background technique [0002] Anthocyanin, also known as anthocyanin, is a kind of natural pigment widely present in plants, belonging to flavonoids, mostly in the form of glycosides, and is the main color-forming substance of plants. As a natural pigment, anthocyanins have high safety performance, and have health functions such as anti-mutation, lowering blood pressure, and protecting the liver, and anthocyanins are called "oral skin cosmetics" in Europe, which can prevent skin wrinkles. Early generation is currently the most effective antioxidant substance in nature. It can not only prevent the early generation of skin wrinkles, but also supplement nutrition and eliminate harmful free radicals in the body. Modern people have found that although the research on antibiotics and vitamins has been very in-depth, they canno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09B61/00C07D311/62
Inventor 杨立飞朱月林刘丹丹应铮峥
Owner NANJING AGRICULTURAL UNIVERSITY
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