Method for extracting procyanidine by utilizing aqueous two-phase system

A two-phase system, two-phase technology, applied in organic chemistry and other directions, can solve the problems of low comprehensive utilization rate of grape seeds, waste of materials, environmental pollution, etc. high water effect

Inactive Publication Date: 2018-02-27
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, despite the rapid development of my country's wine industry, the comprehensive utilization rate of leftovers such as grape seeds is still relative

Method used

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  • Method for extracting procyanidine by utilizing aqueous two-phase system
  • Method for extracting procyanidine by utilizing aqueous two-phase system
  • Method for extracting procyanidine by utilizing aqueous two-phase system

Examples

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Comparison scheme
Effect test

Embodiment 1

[0026] A method for extracting proanthocyanidins utilizing a two-phase partitioning technique, comprising the following steps:

[0027] 1) Material pretreatment: Bake the purchased grape seeds in an oven for 24 hours, then take them out, grind them into powder and seal them in plastic bags for later use.

[0028] 2) Two-phase extraction operation: put 0.5 g of grape seed powder in a two-phase system consisting of 4.5 mL of water, 3.5 mL of ethanol, 2.5 g of ammonium sulfate, and 0.2 g of sodium chloride, and then extract the powder at 3600 r / min. Centrifuge at a rotating speed for 10 minutes, then stand still and separate the phases for 120 minutes, and separate the upper phase to obtain proanthocyanidins.

[0029] Absorbance measurement:

[0030] (1) Dilute the upper phase of the proanthocyanidins prepared in Example 1 three times, keep the lower phase unchanged, put it into a small test tube, and measure its absorbance value at 500 nm.

[0031] (2) Standard curve preparati...

Embodiment 2

[0034] A method for extracting proanthocyanidins utilizing a two-phase partitioning technique, comprising the following steps:

[0035] 1) Material pretreatment: Put the purchased grape seeds in an oven and dry them for 24 hours, then take them out and grind them into powder, and store them in sealed plastic bags for later use.

[0036] 2) Two-phase extraction operation: Take six 50mL centrifuge tubes, numbered 1-6. Add the same amount of ethanol (3mL) and ammonium sulfate (2g) to each centrifuge tube, and add different amounts of distilled water to each centrifuge tube, as shown in the table below. Then add 0.5g of grape seed powder, mix thoroughly, centrifuge at 3600r / min for 10min, place for 120min, collect the upper and lower phases, put the upper and lower phases containing proanthocyanidins into small test tubes, the numbers correspond to the centrifuge tubes, The upper phase was diluted 3 times, and the lower phase was kept unchanged, and then the absorbance values ​​o...

Embodiment 3

[0046] A method for extracting proanthocyanidins utilizing a two-phase partitioning technique, comprising the following steps:

[0047] 1) Material pretreatment: Put the purchased grape seeds in an oven and dry them for 24 hours, then take them out and grind them into powder, and store them in sealed plastic bags for later use.

[0048] 2) Two-phase extraction operation: take four 50mL centrifuge tubes, numbered 1-4. Add the same amount of distilled water (6 mL) and ammonium sulfate (2 g) into each centrifuge tube, and add different amounts of ethanol into each centrifuge tube, as shown in Table 2 below. Then add 0.5g of grape seed powder, mix thoroughly, centrifuge at 3600r / min for 10min, collect the upper and lower phases, put the upper and lower phases containing proanthocyanidins into small test tubes, the numbers correspond to the centrifuge tubes, and separate the upper phase 3-fold dilution, keep the lower phase unchanged, then measure the absorbance value of the upper...

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Abstract

The invention discloses a method for extracting procyanidine from grape seeds by utilizing an aqueous two-phase system. The method comprises the following steps: 1) pretreating a material, namely drying grape seeds in a drying oven at the temperature of 50-80 DEG C for 18-36 hours, and grinding into powder; and 2) performing aqueous two-phase extraction, namely adding an aqueous two-phase extraction solution composed of ethanol, ammonium sulfate, distilled water and sodium chloride into a centrifugal device, adding the grape seed powder, fully mixing, centrifuging at a rotating speed of 3000-6000 revolutions per minute for 10-15 minutes, and standing for extracting, thereby obtaining procyanidine, wherein the aqueous two-phase extraction solution is composed of 30 wt%-35wt% of ethanol, 20wt%-25wt% of ammonium sulfate, 1.8 wt%-2.2wt% of sodium chloride and the balance of distilled water; and a mass ratio of the grape seed powder to the aqueous two-phase extraction solution is 1:(16-30). The method is rapid in phase separation and low in time consumption, the moisture content of the extraction system is high, an extract can be effectively prevented from being inactivated or denatured, the separation process is complete and economic and is simple in operation, and enlarged production and continuous operations are facilitated.

Description

technical field [0001] The invention relates to the field of proanthocyanidin extraction, in particular to a method for extracting proanthocyanidin using a two-phase system. Background technique [0002] Studies have shown that proanthocyanidins have strong antioxidant properties and are widely found in plants. Their function is to prevent the oxidation of easily oxidized components in plants. E , V C are strong, respectively V E 50 times of V C 20 times. The proanthocyanidins in grape seeds also have anti-inflammatory, anti-mutation, hypolipidemic, anti-radiation, cancer prevention, cardiovascular and cerebrovascular disease prevention and cosmetic effects. Grape seeds are an important resource for the extraction of proanthocyanidins, and foreign countries have made reasonable use of them. However, despite the rapid development of my country's wine industry, the comprehensive utilization rate of leftovers such as grape seeds is still relatively low. Most of them are sim...

Claims

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Application Information

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IPC IPC(8): C07D311/62
CPCC07D311/62
Inventor 吴疆冀学栋田申红王少侠
Owner TIANJIN AGRICULTURE COLLEGE
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