Cell-biomaterial composite support frame, and preparation method and application thereof

A technology of composite scaffolds and biomaterials, applied in liquid material additive processing, manufacturing, additive processing, etc., can solve the problems of low adhesion rate, inability to provide a three-dimensional support environment for cell growth, and large scaffold pore size, etc. Achieve the effect of being suitable for industrial production and improving the efficiency of 3D printing

Active Publication Date: 2017-04-19
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, there are many methods for preparing tissue engineering scaffolds. Among them, although the mainstream three-dimensional printing technology has realized the manufacture of scaffolds of any shape on demand, the pore size of the constructed scaffolds is relatively large, and microscopic pores cannot be obtained. The three-dimensional support environment for cell growth, the adhesion rate of cells on the three-dimensional support is low, and most of the cells fall into the culture dish when seeding

Method used

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  • Cell-biomaterial composite support frame, and preparation method and application thereof
  • Cell-biomaterial composite support frame, and preparation method and application thereof
  • Cell-biomaterial composite support frame, and preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0129] A method for preparing a cell-biological material composite scaffold, comprising the following steps:

[0130] 1. Prepare scaffold matrix precursor slurry:

[0131]Inorganic nanoclay-based hydrogel is used as the precursor slurry of the scaffold matrix of the cell-biomaterial composite three-dimensional scaffold, and the preparation of the scaffold matrix precursor slurry is as follows:

[0132] Get the polyethylene glycol macromolecular cross-linking agent modified by the above double bond, Laponite XLG laponite clay, UV photoinitiator 2-hydroxyl-2-methyl-1-phenyl-1-propanone and water to mix at room temperature , to obtain the scaffold matrix precursor slurry, the viscosity of which is 160Pa·s, wherein the above-mentioned raw materials are mixed according to the following mass percentages:

[0133] Polyethylene glycol diacrylate (molecular weight Mn=4000 of polyethylene) 20%

[0134] Laponite XLG clay: 5%

[0135] UV photoinitiator: 0.05%

[0136] Water: 74.95%. ...

Embodiment 2

[0149] A method for preparing a cell-biological material composite scaffold, comprising the following steps:

[0150] 1. Prepare scaffold matrix precursor slurry:

[0151] Inorganic nanoclay-based hydrogel is used as the precursor slurry of the scaffold matrix of the cell-biomaterial composite three-dimensional scaffold. The viscosity of the precursor slurry is 150 Pa s, including the following raw material components in mass percentage:

[0152] Crosslinking agent (methacrylic acid modified gelatin): 20%;

[0153] Laponite XLG clay: 7%;

[0154] UV photoinitiator: 0.05%;

[0155] Water: 72.95%;

[0156] Wherein, the ultraviolet photoinitiator is 1-[4-(2-hydroxyethoxy)-phenylene]-2-hydroxy-2', 2'-dimethylethanone (Irgacure2959).

[0157] 2. Prepare viscous cell suspension:

[0158] Dissolve hyaluronic acid in water to obtain a hyaluronic acid solution, resuspend human osteoblasts in the hyaluronic acid solution, and obtain a viscous cell suspension with a viscosity of 80P...

Embodiment 3

[0166] A method for preparing a cell-biological material composite scaffold, comprising the following steps:

[0167] 1. Prepare scaffold matrix precursor slurry:

[0168] Sodium alginate-based hydrogel is used as the precursor slurry of the scaffold matrix of the cell-biomaterial composite three-dimensional scaffold. The viscosity of the precursor slurry is 130 Pa s, including the following raw material components in mass percentage:

[0169] Crosslinking agent (polyvinyl alcohol modified with methacrylic acid): 20%;

[0170] Sodium alginate: 10%;

[0171] Water: 70%.

[0172] 2. Prepare viscous cell suspension:

[0173] Dissolve hyaluronic acid in water to obtain a hyaluronic acid solution, resuspend human bone marrow stem cells in the hyaluronic acid solution to obtain a viscous cell suspension with a viscosity of 200 Pa·s, in the viscous cell suspension, the mass fraction of hyaluronic acid 30%, the cell density is 2 x 10 6 individual / mL.

[0174] 3. Print composite ...

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Abstract

The invention provides a cell-biomaterial composite support frame. The composite support frame comprises a support frame base body and viscous cell suspension capable of slowly releasing cells, wherein the composite support frame forms the AC<n>B distribution formation from bottom to top, wherein the A is a support frame base body layer; the B is a viscous cell suspension layer; the C is a mixing layer formed by the viscous cell suspension through crossed distribution in support frame base body gaps; the n is a positive integer from 3 to 20; the viscous cell suspension comprises cells, cell carrying carriers and water; and the carriers are biocompatible viscous materials. The distribution density of the ells on the composite support frame is controllable; the adherence rate and the cell survival rate are high; and the problems of weak tissue regeneration promoting capability, weak proliferation and differentiation and slow growth of the cells on a three-dimensional composite support frame can be solved. The invention also provides a preparation method and application of the composite support frame.

Description

technical field [0001] The invention relates to the technical field of biological materials, in particular to a cell-biological material composite scaffold and its preparation method and application. Background technique [0002] Tissue engineering is an emerging discipline that applies the principles and technologies of life science and engineering to research and develop biological substitutes for repairing, maintaining, and promoting the functions and forms of various tissues or organs of the human body after damage. Among them, the selection and construction of biomaterial-based scaffold materials is the key link. Normal human cells are planted on biocompatible scaffold materials, and after they proliferate and differentiate to a certain extent on the scaffold, the adhesion Cell scaffolds are implanted in tissue defect or loss sites to achieve the purpose of tissue repair. Ideal tissue engineering scaffold materials should not only have excellent biocompatibility, bioac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/24A61L27/22A61L27/20A61L27/02B29C64/106B33Y10/00B33Y70/00
CPCA61L27/025A61L27/20A61L27/222A61L27/24A61L27/3808A61L27/3817A61L27/3821A61L27/3834A61L2430/02B33Y30/00B33Y70/00
Inventor 阮长顺翟欣昀吕维加潘浩波
Owner SHENZHEN INST OF ADVANCED TECH
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