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Cellular biological technique, reagent kits and preparation device

A kit and cell technology, applied in the direction of biological testing, preparation of test samples, biochemical equipment and methods, etc., can solve problems that are difficult to distinguish, cumbersome and complicated, and difficult to carry out at the same time

Inactive Publication Date: 2007-02-28
赵翀
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In this case, the problem with the traditional cell smear test is that it is difficult to conduct simultaneous tests on more than two or more different tissue cells due to the difficulty in distinguishing them.
In this case, the problem with the traditional solid-phase adsorption test is that it is difficult to carry out simultaneous tests for more than three different substances to be detected because it is difficult to distinguish them.
That is to say, with the traditional solid phase adsorption test method, the amount of the detected substance to be detected or analyzed in one test is limited or restricted by the solid phase system.
For example, the traditional method is to first purify a large number of different biological probes, such as drug molecules, protease molecules, growth factors and receptors, antigens and antibodies with different specificities, through separation and purification techniques and processes. , and then immobilize the biological probes on the surface of solid-phase substrates such as different microwell plates or microbeads, respectively react with the corresponding chemical substances, antibodies or / and antigens in the sample, and then perform detection and analysis respectively, so If more than a dozen components are to be detected, more than a dozen different experiments are required, which is extremely cumbersome and complicated

Method used

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  • Cellular biological technique, reagent kits and preparation device
  • Cellular biological technique, reagent kits and preparation device
  • Cellular biological technique, reagent kits and preparation device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment I

[0089] Example I: Application in monoclonal antibody development.

[0090] In the research of mouse monoclonal antibodies, it is usually necessary to identify the tissue and cell distribution of the antigen target molecules recognized by the antibodies, such as various receptor molecules or CD molecules on the cell surface, etc. The specific operation steps in the implementation are as follows:

[0091] 1. Glass slide treatment: Strictly cleaned glass slides were dipped in xylene, then rinsed with 70% ethanol and dried; -Aminopropyltriethoxysilane) for 30 seconds, rinse with anhydrous acetone and distilled water sequentially, and dry.

[0092] 2. Loading the slides: Put the processed glass slides into the grooves of the bottom tray of the cell sheet preparation device, followed by the sealing gasket / ring, the through-hole plate, and then tighten the fastening device for later use.

[0093] 3. Preparation of cell sheets: inoculate the adherent growth of different human tumor c...

Embodiment II

[0099] Example II: Application in prenatal and postnatal care.

[0100] The expression of eukaryotic genes and viral proteins in prokaryotic host cells is efficient and inexpensive. However, the expressed proteins often have low or no activity due to incorrect folding or low efficiency. In particular, post-translational processing and modification of proteins cannot be carried out by prokaryotic cells at all. Rubella virus, cytomegalovirus, herpes simplex virus, coxsackie virus and Toxoplasma gondii are the main pathogens that cause intrauterine infection and fetal malformation; hepatitis B, syphilis and AIDS are infectious diseases that can cause neonatal infection through vertical transmission causative agent of disease. Therefore, the detection and diagnosis of the above pathogenic infection in reproductive age and pregnant women has become an important means to prevent congenital malformations and neonatal infections. The specific steps in the implementation are as foll...

Embodiment III

[0107] Example III: Use in in situ hybridization.

[0108] In situ hybridization experiment is the most commonly used technical method in molecular biology, tissue and pathology research, especially in gene expression regulation and tissue cell localization. The specific operation steps in the implementation are as follows:

[0109] 1. Take the cell sheets treated with DEPC-PBS (phosphate buffered saline prepared with DEPC-treated double-distilled water) and treat them with Triton-X100 for 15 minutes. After washing with DEPC-PBS for 25 minutes, use RNase-free proteinase K. Or pepsin was incubated at 37°C for 10-30 minutes, rinsed with glycine PBS or DEPC-PBS, fixed with paraformaldehyde, rinsed with DEPC-PBS, incubated with acetic anhydride triethanolamine solution, and washed with 2xSSC for 3 minutes;

[0110] 2. Use random primers, nick translation or PCR to label or prepare probes;

[0111] 3. Incubate with probe-free oligonucleotide probe hybridization buffer at 37°C for ...

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Abstract

The invention relates to a new cell biological technique, agent box and prepare device, wherein said invention has high sensitivity, large information amount, simple production, and low cost, while it can analyze different nucleic acids, proteins, and molecules of sample at same time; the invention is characterized in that it adheres different cells on film base or plants them on different micro holes of porous plate, the tested sample is combined with cell or carried biology molecule, and the tested result can be watched by eye or recorded and analyzed by machine. The invention can be used in drug research, biology, etc.

Description

technical field [0001] The invention relates to a novel cell biotechnology, a kit and a preparation device. The technology is sensitive, rapid, informative, and has a simple preparation method and low cost. It can simultaneously analyze the distribution and expression regulation of biomolecules such as genes, proteins and antigens in a variety of different tissue cells or cell samples; it can also be used in multiple samples. High-throughput parallel detection and analysis of different chemical substances, biomolecules, microorganisms or cells, etc., can be widely used in biology, medicine, preventive medicine, zoology and botany, agriculture and animal husbandry, food and health, energy and Chemical industry, environmental monitoring, and medical diagnosis and testing. Background technique [0002] Cells and cell lines are the most commonly used in vivo experimental materials in biology, medicine, pharmacy and related fields. They are often used for tissue distribution, ce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50G01N1/28G01N21/78C12Q1/00
Inventor 赵翀
Owner 赵翀
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