Method for detecting release condition of tobacco specific nitrosamines in buccal tobacco product

A technology for tobacco products and nitrosamines, which can be used in measuring devices, instruments, scientific instruments, etc., can solve problems such as lack of technical methods, and achieve the effects of good repeatability and high sensitivity

Active Publication Date: 2011-09-14
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the quality inspection and control of

Method used

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  • Method for detecting release condition of tobacco specific nitrosamines in buccal tobacco product
  • Method for detecting release condition of tobacco specific nitrosamines in buccal tobacco product
  • Method for detecting release condition of tobacco specific nitrosamines in buccal tobacco product

Examples

Experimental program
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Effect test

Embodiment 1

[0037] The flow rate was 0.2 mL / min, the temperature was 37°C, and the release medium was artificial saliva (pH 6.7); TSNAs in the release liquid and residual samples were determined by liquid chromatography-electrospray tandem mass spectrometry.

[0038] The liquid chromatography operating conditions and mass spectrometry detection parameters adopted by liquid chromatography-electrospray tandem mass spectrometry are:

[0039] HPLC parameters: Chromatographic column: Poroshell 120 SB-C18 (3.0 x 100 mm i.d., 2.7 μm, Agilent, USA); column temperature: 50.0 ℃; injection volume: 5 μL; flow rate: 0.30mL / min; mobile phase: A : water (containing 0.1% acetic acid, volume fraction), B: methanol (containing 0.1% acetic acid, volume fraction); pre-equilibration time: 5min; gradient elution conditions are as in the attached Table 1 above.

[0040] Mass spectrometry conditions: ion source: electrospray ionization source (ESI); scan mode: positive ion scan; detection method: multiple reacti...

Embodiment 2

[0043] The flow rate was 0.4mL / min, the temperature was 37°C, and the pH of the artificial saliva was 7.0; the detection of TSNAs in the released liquid was determined by liquid chromatography-electrospray tandem mass spectrometry, and the operating conditions of liquid chromatography and the detection parameters of mass spectrometry were the same. example 1.

[0044] Add artificial saliva with a pH of 7.0 to the solvent storage bottle, turn on the constant temperature tank and constant flow pump, and set the flow rate to 0.4 mL / min. After the system stabilizes to 37 °C, open the glass ground plug and place the sample flat On the stand, cover the ground stopper, and at the same time, collect once every 5 minutes for a total of 60 minutes. After the release solution is collected, take out the residual sample after release and put it into a 50 mL Erlenmeyer flask, add 0.2 mL of 2 μg / mL of internal standard solution (d 4 -NNK,d 4 -NNN,d 4 -NAB and d 4 -NAT mixed solution) an...

Embodiment 3

[0046] The flow rate was 0.2 mL / min for the first 5 minutes and 1 mL / min for the next 55 minutes, the temperature was 37 °C, and the pH of the artificial saliva was 7.0; the detection of TSNAs in its release liquid was performed by high performance liquid chromatography, and the chromatographic parameters were the same as in Example 1

[0047] Add artificial saliva with a pH of 7.0 to the solvent storage bottle, turn on the constant temperature tank and constant flow pump, set the flow rate to 1 mL / min, and after the system stabilizes to 37 °C, open the glass ground plug and place the sample flat Cover the sample holder with a ground stopper, and at the same time, collect once every 5 minutes for a total of 60 minutes. After the release solution is collected, take out the remaining sample after release and put it into a 50 mL Erlenmeyer flask, add 0.2 mL of 2 μg / mL internal standard solution (d 4 -NNK,d 4 -NNN,d 4 -NAB and d 4-NAT mixed solution) and 20 mL of 100 mM ammoni...

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Abstract

The invention relates to a method for detecting release condition of tobacco specific nitrosamines (TSNAs) in a buccal tobacco product. The method is characterized in that: the release condition of the TSNAs in the buccal tobacco product in a human oral cavity is simulated, and release quantity and release speed are quantified timely, so that the in-vitro release speed and release degree of the TSNAs in the buccal tobacco product are detected. TSNAs release collection is realized by an in-vitro release collecting device which can simulate the release process of the TSNAs in the product in the human oral cavity and can collect TSNAs release liquid on line; and the TSNAs content of the release liquid is detected by a liquid chromatogram-electrospray tandem mass spectrum combined technique. The method has the advantages of simulating the release condition of the TSNAs in the oral tobacco product in the human oral cavity, detecting the release speed and the release degree of the TSNAs in various oral tobacco products quickly and accurately, effectively distinguishing release difference of the TSNAs in different products and contributing to popularization and application, along with high repeatability.

Description

technical field [0001] The invention relates to the field of tobacco, in particular to a method for detecting the release of tobacco-specific nitrosamines (tobacco-specific N-nitrosamines, TSNAs) in buccal tobacco products, which can be used for research or measurement of buccal tobacco Provide specific data support for product safety and quality control. Background technique [0002] Bouched-in-mouth tobacco products are tobacco products made by crushing tobacco leaves and then packaging tobacco powders of different particle sizes through water vapor heat treatment and other processes. The biggest difference between this type of product and cigarettes is that it is a smokeless tobacco product. When using it, it is placed between the upper lip of the mouth and the gums. The tobacco components are dissolved in saliva and absorbed through the oral mucosa. [0003] In recent years, due to the restrictions on smoking in public places by governments of various countries, the con...

Claims

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Application Information

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IPC IPC(8): G01N30/88
Inventor 谢剑平孟全新张杰宗永立李鹏
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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