Tobacco product labeling system

a product labeling and tobacco technology, applied in the field of visual indicators, can solve the problems of difficult deciphering for a tobacco user, limited success of nrt in enabling people to quit smoking, etc., and achieve the effect of reducing the exposure of a tobacco consumer to nicotine, less nicotine, and reducing the nicotine conten

Inactive Publication Date: 2006-11-02
VECTOR TOBACCO LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

One drawback of these labeling systems is that they can be very complicated and thus make it difficult for a tobacco user to decipher.
The factors involved with the habitual use of the delivery system are hereinafter referred to as “secondary factors of addiction.” In addition to the fact that conventional NRT does little to quell the secondary factors of addiction, NRT has had only limited success in enabling people to quit tobacco use.

Method used

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  • Tobacco product labeling system

Examples

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example 1

Isolation and Sequencing

[0173] TobRD2 cDNA (Conkling et. al., Plant Phys. 93, 1203 (1990)) encodes QPTase, which is predicted to be a cytosolic protein. Comparisons of the NtQPT1 amino acid sequence with the GenBank database revealed limited sequence similarity to certain bacterial and other proteins; quinolate phosphoribosyl transferase (QPTase) activity has been demonstrated for the S. typhimurium, E. coli, and N. tabacum genes. The NtQPT1 encoded QPTase has similarity to the deduced peptide fragment encoded by an Arabidopsis EST (expression sequence tag) sequence (Genbank Accession number F20096), which may represent part of an Arabidopsis QPTase gene.

example 2

Transformation of Tobacco Plants

[0174] DNA of the QPTase gene, in antisense orientation, is operably linked to a plant promoter (CaMV 35S or TobRD2 root-cortex specific promoter) to produce two different DNA cassettes: CaMV35S promoter / antisense QPTase-encoding gene and TobRD2 promoter / antisense QPTase-encoding gene.

[0175] A wild-type tobacco line and a low-nicotine tobacco line are selected for transformation, e.g., wild-type Burley 21 tobacco (Nic1+ / Nic2+) and homozygous Nic1- / Nic2-Burley 21. A plurality of tobacco plant cells from each line are transformed using each of the DNA cassettes. Transformation is conducted using an Agrobacterium vector, e.g., an Agrobacterium-binary vector carrying Ti-border sequences and the nptII gene (conferring resistance to kanamycin and under the control of the nos promoter (nptII)).

[0176] Transformed cells are selected and regenerated into transgenic tobacco plants called Ro. The Ro plants are grown to maturity and tested for levels of nicotin...

example 3

Tobacco Having Reduced Nicotine Levels

[0178] Tobacco of the variety Burley 21 LA was transformed with the binary Agrobacterium vector pYTY32 to produce a low nicotine tobacco variety, Vector 21-41. The binary vector pYTY32 carried the 2.0 kb NtQPT1 root-cortex-specific promoter driving antisense expression of the NtQPT1 cDNA and the nopaline synthase (nos) 3′ termination sequences from Agrobacterium tumefaciens T-DNA. The selectable marker for this construct was neomycin phosphotransferase (nptII) from E. coli Tn5 which confers resistance to kanamycin, and the expression nptII was directed by the nos promoter from Agrobacterium tumefaciens T-DNA. Transformed cells, tissues, and seedlings were selected by their ability to grow on Murashige-Skoog (MS) medium containing 300 μg / ml kanamycin. Burley 21 LA is a variety of Burley 21 with substantially reduced levels of nicotine as compared with Burley 21 (i.e.. Burley 21 LA has 8% the nicotine levels of Burley 21, see Legg el al., Can J G...

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Abstract

The disclosed invention relates to visual content indicators of a tobacco product. More specifically, the invention includes labeling systems that provide at least one visual indicator of a tobacco product, methods for using the systems, and tobacco products that display one or more visual indicators of content. The present invention generally relates to tobacco products, which have a reduced amount of nicotine and tobacco specific nitrosamines (TSNAs), and contain one or more nicotine substitutes. The use of these tobacco products to reduce the exposure of an individual to nicotine, as well as, facilitating tobacco-use cessation is disclosed.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation of and claims the benefit of priority to U.S. patent application Ser. No. 11 / 393,176, filed Mar. 30, 2006, which is a continuation of and claims the benefit of priority to PCT / US2004 / 029953, which designates that United States and was published in English, filed Sep. 13, 2004, which claims the benefit of priority to U.S. Provisional Application No. 60 / 509,042, filed Oct. 2, 2003; all of the above-referenced applications are hereby expressly incorporated by reference in their entireties.FIELD OF THE INVENTION [0002] The present invention relates to visual indicators that denote the presence or content level of one or more substances found in a tobacco product. More specifically, tobacco product labeling systems that provide at least one visual content indicator of a tobacco product, methods for using these systems, and tobacco products that display one or more visual indicators of content are disclosed....

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A24F47/00A24C1/42A24C5/60A24D3/04A24D3/06G09F
CPCA24B3/12A24B15/10A24B15/20A24B15/243A24B15/245A24C5/601A24D1/002A24D1/025A24D1/16
Inventor REICH, SUSANCONKLING, MARKZELSON, STEVE
Owner VECTOR TOBACCO LLC
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