Soil hydroxyl amine reductase activity detection coloration process

A technology of activity detection and reductase, which is applied in biochemical equipment and methods, measurement/inspection of microorganisms, material analysis through observation of the influence of chemical indicators, etc., can solve large system errors, reduce analysis timeliness, hydroxylamine Insufficient measurement accuracy of reductase activity and other problems, to achieve the effects of high detection accuracy and precision, high sensitivity, high accuracy and precision

Inactive Publication Date: 2008-06-04
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

Its shortcoming is that the concentration of hydroxylamine measured by this color development method is lower than that measured by iodometric method, which often forms a large systematic error
The hydroxylamine reductase activity determined by this method is also lacki

Method used

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  • Soil hydroxyl amine reductase activity detection coloration process
  • Soil hydroxyl amine reductase activity detection coloration process
  • Soil hydroxyl amine reductase activity detection coloration process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Soil 1 is red soil, collected from Yingtan Experimental Station, Jiangxi, Chinese Academy of Sciences; soil 2 is meadow brown soil, collected from Shenyang Ecological Station, Chinese Academy of Sciences; soil 3 is paddy soil, collected from Yangzhou Fine Seed Farm, Jiangsu. The hydroxylamine reductase activity in three soils is now measured by spectrophotometry, and the color development and colorimetry are carried out by the color development method of the present invention.

[0039] Take 0.5g of air-dried soil and place it in a 100ml bottle with a ground stopper, add 20mg of calcium carbonate, and mix carefully. Then 1 ml of 0.5% hydroxylamine hydrochloride solution and 1 ml of 1% glucose solution were added. Extract the air in the bottle under the pressure of 10-12mm mercury column. Shake the bottle carefully and place it in a 30°C incubator for 5h. Sterilized soil plus reagent was used as a control. After cultivating, add 50ml of distilled water and 2ml of alumi...

Embodiment 2

[0045] The three kinds of soils used in this example are: No. 1 is the control, that is, ordinary soil that has been cultivated at 25°C for more than 24 hours without any treatment and without adding any human soil additives; Dimethylpyrazole phosphate and cultivated at 25°C for more than 24h; No. 3 is the soil added with nitrification inhibitor 1-hydroxymethyl-3,5-dimethylpyrazole and cultivated at 25°C for more than 24h, soil The water content is 20%. No. 2 and No. 3 soils with nitrification inhibitors added, the addition amount of both inhibitors is 1.15mg·kg -1 earth. The activities of three soil hydroxylamine reductases were detected by the above method.

[0046] The specific analysis process for each soil is as follows:

[0047] Take 1.0g of air-dried soil and place it in a 100ml bottle with a ground stopper, add 10mg of calcium carbonate, and mix carefully. Then 2 ml of 0.5% hydroxylamine hydrochloride solution and 2 ml of 1% glucose solution were added. Extract th...

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Abstract

The invention relates to a color development method for detecting the soil hydroxylamine reductase activity, in particular to a method that the extracting solution after the soil culture is added in with the ferric iron salt solution and the Alfa-Alfa'bipyridyl solution and has the color developed in the subacidity environment, and then the hydroxylamine concentration of the extracting solution are colorimetric-determined by a spectrophotometric method. The hydroxylamine concentration of the culture solution is acquired by comparing the extinction value of the extracting solution with the standard curve; as a result, the soil hydroxylamine reductase activity is worked out. The color reaction is carried out at the normal temperature, the acquired solution shows orange, and the color comparison wave length is 520nm. If the color comparison can not be completed in 40 minutes, the masking agent should be added in order to prevent the side reaction. Compared with the traditional color development method, the improved color development method has the advantages of high sensitivity, small system error, high accuracy and precision and little use level of the color developing agent, more accurate and reliable analysis result and better analysis reproducibility.

Description

technical field [0001] The invention relates to a color development method in the process of detecting hydroxylamine reductase activity in soil by using spectrophotometry, in particular to a color development method for indirect determination of hydroxylamine concentration in soil culture solution by using a complex color development method. Background technique [0002] Hydroxylamine reductase in the soil can reduce the intermediate product hydroxylamine formed in the process of nitrogen metabolism (nitrite reduction or ammonia oxidation) in the soil to ammonia, and the reduced compound in the soil can be used as a hydrogen donor. [0003] <chemistry num="0001"> <chem file="200610134408_CML001.XML" / > < / chemistry> [0004] Therefore, the activity of hydroxylamine reductase in soil affects the ammonia volatilization loss of nitrogen in the process of soil nitrogen metabolism, and indirectly affects the use efficiency of nitrogen fertilizer. The activity...

Claims

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Application Information

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IPC IPC(8): G01N21/78C12Q1/26
Inventor 武志杰史云峰陈利军王殳屹隽英华
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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