16 results about "Nitrate reductase (NADH)" patented technology

This invention provides compositions and treatment methods for reducing hyperphenylalaninemia. [Solution] A genetically modified bacterium is provided, comprising: a) one or more genes encoding phenylalanine ammonia lyase (PAL), which are operably linked to a promoter that is not naturally associated with the PAL gene and can be directly or indirectly induced; b) one or more genes encoding a phenylalanine transporter, which are not naturally associated with the phenylalanine transporter gene and can be operably linked to a promoter that is directly or indirectly induceable; and c) one or more genes encoding mutant fumarate and nitrate reductase (FNR), which are not naturally associated with the FNR gene and can be operably linked to a promoter that is directly or indirectly induceable.

This invention belongs to the field of organic agriculture, specifically a biological agent for promoting wheat photosynthesis and its preparation method. Addressing the problems of existing biological agents having relatively simple components, low absorption and utilization efficiency by wheat ears, and a tendency to reduce nitrate reductase activity, nitrogen utilization, and chlorophyll content in wheat leaves, the following solution is proposed: the biological agent comprises the following raw materials in parts by weight: 50-65 parts of yield-enhancing amine, 15-20 parts of choline chloride, 10-15 parts of diethylaminoethanol hexanoate, 5-10 parts of brassinolide, 3-5 parts of p-chlorophenoxyacetic acid, 1-3 parts of calcium diphenylureasulfonate, 2-5 parts of trace agents, and 2-4 parts of ethylene glycol styraldehyde. The biological agent of this invention can promote better photosynthesis in wheat, enhance nitrate reductase activity, and improve nitrogen utilization.

This invention provides genes that enhance crop nitrogen metabolism in acidic soils and their applications, belonging to the field of genetic engineering technology. This invention discovers transcription factors... OsART1 It not only participates in regulating the aluminum toxicity response but also integrates the regulation of key processes in nitrogen uptake and assimilation, making it a core regulatory factor connecting acidic soil adaptability with nitrogen metabolism coordination. The transcription factor... OsART1 By directly activating nitrogen metabolism genes such as nitrate reductase OsNR2 The expression of [a specific ingredient] is used to synergistically enhance the nitrogen metabolism capacity and nitrogen use efficiency of rice in acidic soils. This invention [is described in the original text]. OsART1 By enhancing nitrogen assimilation and promoting root growth, it has become a key regulatory factor affecting nitrogen utilization and yield formation in rice under acidic environments.

This invention discloses a method for promoting the biodegradation of N,N-dimethylformamide and simultaneous denitrification. The method constructs a zero-valent iron (ZFE)-coupled biological treatment system, which not only accelerates electron transfer efficiency but also significantly increases the activity of DMF-degrading enzymes and nitrate reductases, synergistically promoting electron transfer and microbial metabolic activity, thereby enhancing the removal of DMF and nitrate nitrogen. Compared to biological treatment systems that only add activated sludge without adding ZFE, adding ZFE significantly improves the removal efficiency of DMF and nitrate nitrogen, making it suitable for the treatment of large-scale polluted water bodies or industrial wastewater.

The invention belongs to the technical field of forest tree genetic engineering breeding, and particularly relates to a CbuLBD19 gene for regulating and controlling root system development and nitrogen absorption and utilization of catalpa bungei and application of the CbuLBD19 gene. The nucleotide sequence of the CbuLBD19 gene is as shown in SEQ ID No.7, and the amino acid sequence coded by the CbuLBD19 gene is as shown in SEQ ID No.8. It is found that overexpression of the CbuLBD19 gene can cause reduction of the total root length and the root biomass of catalpa bungei. Meanwhile, the net absorption rate of nitrate ions in the root system, the activity of nitrate reductase (NR) and the content of amino acid are reduced, and the comprehensive expression is that nitrogen metabolism is reduced. The negative regulation and control function of the CbuLBD19 gene on the nitrogen absorption and utilization of the catalpa bungei is defined, and a technical route for improving the nitrogen absorption and utilization of the catalpa bungei by reducing the expression of the CbuLBD19 gene is provided.

This invention provides a nitrate reductase gene and its application as a target for regulating plant stress resistance. The invention discloses a novel nitrate reductase gene, named NR1.2, that regulates nitrogen use efficiency in plants. NR1.2 is closely related to drought tolerance in plants; reducing the expression / activity of NR1.2 in plants results in decreased seedling height and increased drought tolerance. Therefore, NR1.2 can be used as a target for regulating drought tolerance in plants, and materials or methods for downregulating NR1.2 can be applied to improve plant varieties.

The present disclosure relates to an L-arginine-producing microorganism in which the activity of a regulator of nitrate reductase operon expression is attenuated and the activity of glutamine synthetase is enhanced, and to a method for producing L-arginine using same, wherein the L-arginine-producing microorganism, in which the activity of the regulator of nitrate reductase operon expression is attenuated and the activity of glutamine synthetase is enhanced, has been confirmed to exhibit increased L-arginine production capability compared to a parent strain, and thus can be widely used for L-arginine production.

The invention belongs to the technical field of medicinal chemistry, and particularly relates to a tripterine prodrug compound as well as a preparation method and application thereof. The tripterine prodrug compound provided by the invention has a structure as shown in a formula I. The tripterine prodrug compound designed by utilizing the unique high expression characteristic of nitrate reductase in a tumor microenvironment can be specifically activated by the nitrate reductase at a tumor part, so that accurate release of tripterine at the tumor part is realized; the tripterine prodrug compound is kept relatively stable in normal tissues, so that the toxic effect on tissues such as normal livers is greatly reduced, and an innovative and potential solution is provided for solving the hepatotoxicity problem of the tripterine and improving the anti-tumor treatment effect of the tripterine; and breakthrough is hopefully brought to tumor treatment and the clinical application value is improved. Formula I.

The invention provides bifidobacterium breve NKU BB 1-13 and application thereof, and belongs to the technical field of microorganisms. The invention provides a bifidobacterium breve NKU BB 1-13, and the preservation number of the bifidobacterium breve NKU BB 1-13 is GDMCC No: 65957. In the invention, the bifidobacterium breve NKU BB 1-13 cannot generate nitrate reductase, shows negative ornithine and lysine decarboxylase, has no hemolytic activity, and is a safe strain. The bifidobacterium breve NKU BB 1-13 has the characteristics of acid resistance and cholate resistance, and can tolerate the digestion environment of gastrointestinal tracts. The bifidobacterium breve NKU BB 1-13 can relieve oxidative stress and inflammation of intestinal tracts in the aging process, meanwhile, can promote goblet cells to secrete intestinal barrier protein and improve the intestinal barrier function, and then can improve intestinal health in the aging process.

Additive mixture for fermentation liquids, containing at least the following components: (A) at least one type of activated carbon containing at least one absorbed or adsorbed or absorbed and adsorbed inorganic nitrate, (B) (b1) NADH and / or NADPH nitrate reductase obtained from plants or yeasts or (b2) at least one type of denitrifying bacteria in free form or (b3) at least one type of denitrifying bacteria carried on and / or in at least one type of activated carbon or (b4) at least one type of denitrifying bacteria in free form and supported on and / or in at least one type of activated carbon or (b5) NADH and / or NADPH nitrate reductase obtained from plants or yeasts and at least one type of denitrifying bacteria in free form or (b6) NADH and / or NADPH nitrate reductase derived from plants or yeasts and at least one type of denitrifying bacteria carried on and / or in at least one type of activated carbon or (b7) NADH and / or NADPH nitrate reductase derived from plants or yeasts, at least one type of denitrifying bacteria in free form and at least one type of denitrifying bacteria carried on and / or in at least one type of activated carbon each in a total quantity that is insufficient to replace the nitrate present in (A) according to equation (I) NO3 - +2 H + + 2 e - = NO2 - + H2O (I) to reduce completely to nitrite, and (C) the bacterium Candidatus Brocadia Anammoxidans in free form or supported on and / or in at least one type of activated carbon or in free form and supported on and / or in at least one type of activated carbon, each in a total quantity sufficient to remove the nitrite resulting according to equation (I) and the ammonia present in the fermentation liquid according to equation (II): NH4 + + NO2 - = N2 + H2O (II), to proportionate to nitrogen.

The invention relates to the technical field of synthetic biology, and discloses an antibiotic-label-free genome traceless editing method in blue-green algae, which comprises the following steps: S1, constructing a chassis cell: knocking out a nitrate reductase gene of the blue-green algae to obtain a mutant strain which can only grow by taking nitrite as a sole nitrogen source; s2, constructing a plasmid platform: constructing at least one recombinant plasmid containing a nitrate reductase gene, a homologous arm and a target gene insertion site; s3, target gene insertion: cloning an exogenous target gene to the target gene insertion site of the recombinant plasmid in the step S2 to obtain a recombinant expression plasmid containing the target gene; s4, transformation and screening: introducing the recombinant expression plasmid in the step S3 into the chassis cell in the step S1, and screening a positive transformant through a nitrate-containing culture medium to realize traceless insertion of an exogenous gene; and S5, repeating the steps S2, S3 and S4, and realizing continuous traceless insertion of a plurality of exogenous genes by alternately using a culture medium containing nitrate or nitrite.

The invention discloses an OsSCL7 gene and application thereof in improving nitrogen utilization efficiency of rice, and belongs to the technical field of plant genetic engineering, and a nucleotide sequence of the OsSCL7 gene is shown as SEQ ID No: 1. With rice ZH11 as the background, it is found that rice with the OsSCL7 gene overexpressed is higher than wild type rice ZH11 in plant height under the nitrogen deficiency condition, and meanwhile the ammoniacal nitrogen content, the nitrate nitrogen content, the nitrate reductase enzyme activity and the glutamine synthetase enzyme activity are all remarkably higher than those of the wild type rice ZH11. Therefore, the OsSCL7 gene can participate in regulation and control of nitrogen utilization of rice, is of great significance to nitrogen fertilizer application reduction, rice agricultural production cost reduction and agricultural sustainable development, and provides an important gene resource for cultivation of rice varieties with nitrogen deficiency stress resistance.

There is disclosed herein a Nicotiana tabacum plant cell comprising: (a) a polynucleotide sequence encoding a NIA1 nitrate reductase polypeptide comprising a contiguous polypeptide sequence of SEQ ID NO: 4, wherein the serine at position 4 of SEQ ID NO: 4 is substituted for an amino acid that reduces nitrate levels in the plant cell as compared to a control plant cell; (b) a polypeptide sequence encoded by the polynucleotide sequence set forth in (a); or (c) a construct, vector or expression vector comprising the polynucleotide sequence set forth in (b). Double mutants are also disclosed containing, for example, a S521N mutation in a Nicotiana tabacum NIA1 nitrate reductase polypeptide and a M527I mutation in a Nicotiana tabacum NIA2 nitrate reductase polypeptide.

The application belongs to the technical field of forest tree genetic engineering breeding, and more particularly relates to a CbuLBD19 gene for regulating root development and nitrogen absorption and utilization of a Catalpa bungeana and application thereof. The nucleotide sequence of the CbuLBD19 gene is shown as SEQ ID No. 7, and the amino acid sequence encoded by the CbuLBD19 gene is shown as SEQ ID No. 8. The application finds that overexpression of the CbuLBD19 gene can lead to reduction of total root length and root biomass of the Catalpa bungeana. Meanwhile, the overexpression can lead to reduction of net absorption rate of nitrate ions, nitrate reductase (NR) activity and amino acid content in the root system, and the Catalpa bungeana shows a comprehensive decrease in nitrogen metabolism. The function of the CbuLBD19 gene in negative regulation of nitrogen absorption and utilization of the Catalpa bungeana is clarified, and a technical route for improving nitrogen absorption and utilization of the Catalpa bungeana by reducing expression of the CbuLBD19 gene is provided.

The present disclosure relates to an L-arginine-producing microorganism in which the activity of a regulator of nitrate reductase operon expression is attenuated and the activity of glutamine synthetase is enhanced, and to a method for producing L-arginine using same, wherein the L-arginine-producing microorganism, in which the activity of the regulator of nitrate reductase operon expression is attenuated and the activity of glutamine synthetase is enhanced, has been confirmed to exhibit increased L-arginine production capability compared to a parent strain, and thus can be widely used for L-arginine production.

The application discloses a soil staphylococcus with high-temperature resistance and smoking resistance, and application thereof, and belongs to the technical field of microbial application and fermented meat products. The soil staphylococcus has a preservation number of CGMCC No. 34994, is named XUCSB 058, has nitrate reductase, catalase, proteolytic enzyme and lipolytic enzyme, can produce acid by utilizing glucose, and has the characteristics of acid resistance, salt resistance, nitrite resistance, low-temperature resistance, non-hemolysis, non-stick production, non-gas production, non-biogenic amine production, non-NH3 production, non-H2S production and the like. The soil staphylococcus has the characteristics of high-temperature resistance and smoking resistance, can promote the persistent formation of color and flavor of smoking fermented meat (such as bacon, sausage, smoked ham and the like), and has the potential of being safely used in the production of various smoking fermented meat (such as bacon, sausage, smoked ham and the like).