The invention provides a degrading bacterial agent for eliminating norfloxacin residues in waste water, which belongs to the technical field of biological high technology. The strain used was Gram-negative bacteria NOR‑36, which was identified as Acinetobacter . calcoaceticus. It was deposited in the General Microbiology Center of China Microbial Cultures Preservation Management Committee (CGMCC for short, address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, Zip code 100101), on February 9, 2015, with a deposit number CGMCC NO.10540, identified as Acinetobacter calcoaceticus. The main biological characteristics are negative Gram stain, smooth, short rod-shaped colonies, positive for contact oxidase, and negative for nitrate reductase. The direct application of degrading bacteria products can reduce the residual amount of norfloxacin in water by more than 90%.