Staphylococcus equi FS310 and application thereof
A technology of FS310 and Staphylococcus, which is applied in the direction of bacteria, microorganisms, and methods based on microorganisms, can solve the problems that have not been reported on Staphylococcus hormonus, achieve good development and application prospects, improve texture and flavor, and facilitate industrial production Effect
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Embodiment 1
[0027] Example 1: Isolation, screening and identification of Staphylococcus equigastrium FS310
[0028] 1. Collection of samples
[0029] Take a few small pieces of good-flavored Xuanwei ham purchased from Xuanwei, Yunnan, mix them together, 500g in total, label them, and place them in a sampling box with dry ice. After the samples arrive in the laboratory, store them in a refrigerator at 4°C, and store them in the next day. days for sample processing;
[0030] 2. Enrichment culture of the target strain
[0031] The collected samples were minced with a meat grinder, and the meat grinder was sterilized in advance, and 25g of the minced meat tissue was transferred to a 225mL sterile beaker containing 0.85% sterile saline, and the homogenizer was used at 8000r / min , homogenized for 10 min, the homogenized solution was transferred to a sterilized Erlenmeyer flask, oscillated on a shaker for 30 min, and rested for 5 min for gradient dilution. Select 10 -3 、10 -4 、10 -5 Three ...
Embodiment 2
[0039] Example 2: Research on the Functional Characteristics of Staphylococcus equini FS310
[0040] (1) Determination of the growth curve of Staphylococcus equini FS310
[0041] Inoculate Staphylococcus hormones FS310 strain in MSA liquid medium, culture at 30°C for 24h, use a 723 spectrophotometer at a wavelength of 600nm, and use a blank medium as a control, measure its OD value every 4h, record the results and draw a growth curve ( Figure 4 ).
[0042] (2) Nitrite tolerance
[0043] Staphylococcus hormonus FS310 was inoculated in NaNO containing 150 mg / kg at an inoculum volume of 1%. 2 In the MSA culture medium, after culturing for 24 hours, the absorbance value was measured at 600nm with the blank culture medium as a control, and the results are shown in Table 2;
[0044] (3) Salt tolerance
[0045]Inoculate Staphylococcus rumen FS310 with 1% inoculum amount and inoculate in MSA medium containing 10% sodium chloride. After culturing for 24 hours, use blank medium as...
Embodiment 3
[0062] Embodiment 3: Microbial Safety Evaluation of Staphylococcus equini FS310
[0063] (1) Hemolysis test: Inoculate Staphylococcus equineus FS310 cultivated overnight on a blood plate by streaking with an inoculation loop. After incubating at 30°C for 24 hours, observe whether there is a hemolysis circle. If there is a hemolysis circle, it is positive, and if there is no hemolysis circle, it is negative. Indicates safety; the experimental results are shown in Table 3;
[0064] (2) Plasma coagulation enzyme test: Add 0.5mL sterile normal saline to each vial containing freeze-dried rabbit plasma to dissolve it completely, add 0.3mL of 24h liquid culture of Staphylococcus hormeus FS310, mix well Mix well, place at 30°C and incubate, observe whether there is coagulation within 2~6 hours, if coagulation is positive, if no coagulation is negative, it is safe, and the experimental results are shown in Table 3;
[0065] (3) Heat-resistant deoxyribonuclease experiment: Inoculate th...
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