Staphylococcus equi FS310 and application thereof

A technology of FS310 and Staphylococcus, which is applied in the direction of bacteria, microorganisms, and methods based on microorganisms, can solve the problems that have not been reported on Staphylococcus hormonus, achieve good development and application prospects, improve texture and flavor, and facilitate industrial production Effect

Active Publication Date: 2022-05-17
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are no relevant reports about Staphylococcus equimus in the fermentation of meat products

Method used

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  • Staphylococcus equi FS310 and application thereof
  • Staphylococcus equi FS310 and application thereof
  • Staphylococcus equi FS310 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Isolation, screening and identification of Staphylococcus equigastrium FS310

[0028] 1. Collection of samples

[0029] Take a few small pieces of good-flavored Xuanwei ham purchased from Xuanwei, Yunnan, mix them together, 500g in total, label them, and place them in a sampling box with dry ice. After the samples arrive in the laboratory, store them in a refrigerator at 4°C, and store them in the next day. days for sample processing;

[0030] 2. Enrichment culture of the target strain

[0031] The collected samples were minced with a meat grinder, and the meat grinder was sterilized in advance, and 25g of the minced meat tissue was transferred to a 225mL sterile beaker containing 0.85% sterile saline, and the homogenizer was used at 8000r / min , homogenized for 10 min, the homogenized solution was transferred to a sterilized Erlenmeyer flask, oscillated on a shaker for 30 min, and rested for 5 min for gradient dilution. Select 10 -3 、10 -4 、10 -5 Three ...

Embodiment 2

[0039] Example 2: Research on the Functional Characteristics of Staphylococcus equini FS310

[0040] (1) Determination of the growth curve of Staphylococcus equini FS310

[0041] Inoculate Staphylococcus hormones FS310 strain in MSA liquid medium, culture at 30°C for 24h, use a 723 spectrophotometer at a wavelength of 600nm, and use a blank medium as a control, measure its OD value every 4h, record the results and draw a growth curve ( Figure 4 ).

[0042] (2) Nitrite tolerance

[0043] Staphylococcus hormonus FS310 was inoculated in NaNO containing 150 mg / kg at an inoculum volume of 1%. 2 In the MSA culture medium, after culturing for 24 hours, the absorbance value was measured at 600nm with the blank culture medium as a control, and the results are shown in Table 2;

[0044] (3) Salt tolerance

[0045]Inoculate Staphylococcus rumen FS310 with 1% inoculum amount and inoculate in MSA medium containing 10% sodium chloride. After culturing for 24 hours, use blank medium as...

Embodiment 3

[0062] Embodiment 3: Microbial Safety Evaluation of Staphylococcus equini FS310

[0063] (1) Hemolysis test: Inoculate Staphylococcus equineus FS310 cultivated overnight on a blood plate by streaking with an inoculation loop. After incubating at 30°C for 24 hours, observe whether there is a hemolysis circle. If there is a hemolysis circle, it is positive, and if there is no hemolysis circle, it is negative. Indicates safety; the experimental results are shown in Table 3;

[0064] (2) Plasma coagulation enzyme test: Add 0.5mL sterile normal saline to each vial containing freeze-dried rabbit plasma to dissolve it completely, add 0.3mL of 24h liquid culture of Staphylococcus hormeus FS310, mix well Mix well, place at 30°C and incubate, observe whether there is coagulation within 2~6 hours, if coagulation is positive, if no coagulation is negative, it is safe, and the experimental results are shown in Table 3;

[0065] (3) Heat-resistant deoxyribonuclease experiment: Inoculate th...

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Abstract

The invention provides staphylococcus equorum FS310 derived from Xuanwei ham, which is in CGMCC (China General Microbiological Culture Collection Center) No.24492. The strain is high in production adaptability (high salt resistance, nitrate resistance and acid resistance), has the biological characteristics of high growth speed, high acid production capacity and the like, and can be used for preparing high-yield acid-resistant bacteria. Due to the protease activity, nitrate reductase activity, relatively strong antioxidant enzyme activity and biological safety, the bacillus amyloliquefaciens has huge application potential; when the staphylococcus equorum FS310 is applied to fermentation of hams, sausages and aquatic products, color development of meat products can be obviously promoted, the residual amount of nitrite is reduced, fat peroxidation is inhibited, the texture and flavor of the meat products are improved, and the staphylococcus equorum FS310 has a good application prospect in production of fermented meat products as a leavening agent for meat.

Description

technical field [0001] The invention belongs to the technical field of food microbes, in particular to a strain of Staphylococcus equini ( Staphylococcus equorum ) FS310 and its application. Background technique [0002] Fermented meat products refer to the use of microbial fermentation under natural and artificial control conditions to produce meat products with special flavor, color and texture, and have a long shelf life. At present, European and American countries have used microbial starters to produce large-scale fermented meat products (such as Spanish Iberian ham, Italian Felino salami, etc.), with various varieties, widely accepted by consumers, huge market potential, and technological advantages. Sophisticated, strong color and fragrance, unique flavor and so on. However, my country's fermented meat products mainly rely on natural fermentation (such as Zhejiang Jinhua ham, Yunnan Xuanwei ham, Sichuan sausage, etc.), the production cycle is long, and is limited by...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L13/70A23L13/40C12R1/44
CPCA23L13/45A23L13/74
Inventor 梁小波商攀飞谭海青王振宇刘贻坦冯应山
Owner KUNMING UNIV OF SCI & TECH
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