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A strain of Pseudomonas stutzeri with aerobic denitrification function and its application

A technology of Pseudomonas stutzeri and aerobic denitrification, applied in the field of microorganisms, can solve the problems of time consumption, complexity of sewage treatment system, low nitrification rate, etc., achieve fast metabolism, overcome intolerance to high organic load , The effect of short film growth time

Active Publication Date: 2021-02-12
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This whole reaction process is not only time consuming but also has low nitrification rates and the complexity of wastewater treatment systems separating aerobic and anoxic zones

Method used

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  • A strain of Pseudomonas stutzeri with aerobic denitrification function and its application
  • A strain of Pseudomonas stutzeri with aerobic denitrification function and its application
  • A strain of Pseudomonas stutzeri with aerobic denitrification function and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: the screening method of strain

[0022] culture medium

[0023] Denitrification enrichment medium: Na 3 C 6 h 5 o 7 2H 2 O 1g / L, KNO 3 1g / L, KH 2 PO 4 1.5g / L, Na 2 HPO 4 0.42g / L, MgSO 4 ·7H 2 O 1.0g / L, CuSO 4 ·5H 2 O 4.0mg / L, FeSO 4 ·7H 2 O 0.7mg / L, FeCl 3 ·6H 2 O7.0mg / L, CoCl 3 ·6H 2O 0.2mg / L, Na 2 MO 4 2H 2 O 3.4mg / L, CaCl 2 2H 2 O 2.0g / L, pH 7.2.

[0024] Bromothymol blue (BTB) identification medium: Na 3 C 6 h 5 o 7 2H 2 O 1g / L, KNO 3 1.0g / L, FeSO 4 ·7H 2 O0.05 g / L, CaCl 2 2H 2 O 0.2g / L, MgSO 4 ·7H 2 O 1.0g / L, 1% BTB 1.0mL / L, pH 6.8.

[0025] Denitrification performance test medium: Na 3 C 6 h 5 o 7 2H 2 O 1.0g / L, KNO 3 1.0g / L, KH 2 PO 4 0.75g / L, MgSO 4 ·7H 2 O 0.4g / L, pH 7.2.

[0026] Ammonia nitrogen degradation performance test medium: NH 4 Cl 0.382g / L, CH 3 COONa 0.7g / L, MgSO 4 ·7H 2 O0.05g / L, K 2 HPO 4 0.2g / L, NaCl 0.12g / L, MnSO 4 4H 2 O 0.01g / L, FeSO 4 0.01g / L, pH 7.2.

[0027] Nitr...

Embodiment 2

[0042] Embodiment 2: Observation of strain morphology

[0043] Such as figure 1 As shown, the purified preferred strain was spread on the BTB solid medium, and the colony morphology of the strain was observed. The results of BTB plate identification show that ZH-14 can carry out denitrification and alkali production on the solid plate with potassium nitrate as the only nitrogen source, increase the pH value of the medium, and turn BTB from green to blue. The single colony of strain ZH-14 on the BTB plate was round, light yellow, smooth, opaque, raised, and easy to stir up in the early stage; light yellow folds were formed on the surface in the late growth period, and the surface area of ​​the colony became larger.

Embodiment 3

[0044] Example 3: Nitrogen removal performance test

[0045] 1. Nitrogen removal performance test of aerobic denitrifying bacteria

[0046] Pick a single colony and inoculate them on the denitrification performance test medium, nitrite degradation test medium and ammonia nitrogen degradation test test medium respectively, and culture at constant temperature and shaking at 30°C and 160r / min for 1 day to obtain the corresponding seed solution.

[0047] Take the above-mentioned bacterial solution and inoculate them according to 1% (v / v) into 250mL Erlenmeyer flasks equipped with 100mL denitrification performance test medium, nitrite degradation test medium and ammonia nitrogen degradation test medium. Three repetitions were carried out at 30°C and 160r / min for constant temperature shaking culture. Among them, the denitrification performance measurement medium, the nitrite degradation performance measurement medium are sampled every 6h, the ammonia nitrogen degradation performanc...

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Abstract

The invention belongs to the field of microorganisms, and discloses a strain of Pseudomonas stutzeri ZH‑14 (Pseudomonas stutzeri ZH‑14) with aerobic denitrification function, and its preservation number is CCTCC NO: M2018730. The above Pseudomonas stutzeri can be applied to degrade ammonia nitrogen or nitrate nitrogen or nitrite nitrogen or COD in nitrogenous sewage. Beneficial effects: Pseudomonas stutzeri ZH‑14 has both aerobic denitrification and heterotrophic nitrification functions, and the degradation process contains extremely low levels of nitrite nitrogen accumulation; it can directly use nitrite for growth and metabolism ;Pseudomonas stutzeri ZH‑14 has a relatively strong periplasmic nitrate reductase activity in a low carbon-to-nitrogen ratio environment, and the aerobic expression is less affected by the type of carbon source used, which solves the problem of using aerobic Carbon source problem in the process of denitrifying bacteria treating nitrogenous sewage.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Pseudomonas stutzeri with aerobic denitrification function and its application. Background technique [0002] In recent years, with the improvement of large-scale breeding of livestock and poultry, the problem of breeding pollution has become increasingly prominent and has become an important bottleneck restricting the development of animal husbandry. Biogas slurry is the product of livestock and poultry sewage treated by anaerobic biogas engineering. Because it contains a lot of organic matter, nitrogen, phosphorus and other pollutants, it cannot be discharged directly, and further treatment is still needed to avoid the impact on the environment. At present, the more common biogas slurry treatment processes include physical, chemical and biological means. Among them, the technology of using microorganisms to treat biogas slurry has played an important role in the purif...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/02C02F3/34C12R1/38C02F101/16
CPCY02W10/10
Inventor 汤江武孙宏李园成王新姚晓红吴逸飞沈琦
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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