Modulation of nitrate levels in plants via mutation of nitrate reductase
A nitrate, reductase technology, applied in the directions of oxidoreductase, plant products, plant genetic improvement, etc., can solve problems such as low nitrite levels, and achieve the effect of overcoming the difficulties of growth and commercialization
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
specific Embodiment approach
[0072] In one aspect, an isolated polynucleotide sequence encoding a nitrate reductase polypeptide comprising the contiguous polypeptide sequence of SEQ ID NO: 5 is provided, wherein methionine is reduced compared to a control plant cell Amino acid substitutions for nitrate reductase activity.
[0073] Suitably, the nitrate reductase polypeptide has the contiguous polypeptide sequence of SEQ ID NO: 7, wherein methionine is substituted with an amino acid that reduces nitrate reductase activity compared to control plant cells.
[0074] Suitably, the isolated polynucleotide sequence encodes a nitrate reductase polypeptide in which methionine is substituted by a different amino acid, preferably an aliphatic non-polar amino acid, more preferably isoleucine .
[0075] Suitably, the aliphatic non-polar amino acid is selected from the group of amino acids consisting of glycine, alanine, proline, isoleucine, leucine or valine.
[0076] Suitably, the aliphatic non-polar amino acid is ...
Embodiment
[0184] M0 seeds of Nicotiana vulgaris AA37 were treated with ethyl methanesulfonate (EMS) at different concentrations and exposure times to generate plant populations with random point mutations. Kill curves were estimated for each treatment at M1 generation, along with lethality, fertility and mosaicism. Selfing of the M1 plants produces seeds of the M2 family to allow the recessive allele to be recovered as homozygotes and the lethal allele to be recovered as heterozygotes. Genomic DNA was extracted from eight M2 plants per family of the EMS mutagenized population and screened for mutants, while M2 plant material and M3 seeds were collected and stored for future analysis. To identify and characterize mutant variants, genomic DNA samples from M2 plants were pooled in groups and screened by sequencing of targeted gene fragments. The tobacco NIA2 gene-specific primers shown in Table 2 were used to amplify the target gene fragment. Mutations in target genes are retrieved by se...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com