Underflow constructed wetland ecological system for disposing rural domestic waste and disposing method

A technology for constructed wetlands and domestic sewage, applied in biological treatment devices, chemical instruments and methods, biological water/sewage treatment, etc. The effect of faster builds, lower costs, optimized operation

Active Publication Date: 2018-02-13
ZHEJIANG METALLURGICAL RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the intermittent operation mode has unstable factors for the treatment of rural domestic

Method used

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  • Underflow constructed wetland ecological system for disposing rural domestic waste and disposing method
  • Underflow constructed wetland ecological system for disposing rural domestic waste and disposing method
  • Underflow constructed wetland ecological system for disposing rural domestic waste and disposing method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1

[0024] Take domestic sewage and mix it with clean water according to the volume ratio of 1:4. Use it as a culture solution to cultivate ungerminated canna seeds. When the root length reaches about 10cm, rinse the roots of the canna so that the root microorganisms are dispersed in the culture solution. The OD of the culture solution 600nm =0.2; filter with 0.22μm filter membrane, take the filter membrane in DNA extraction kit ( In DNAIsolationKit), the extracted DNA product is first used for sample amplification. The PCR primers used the V3-V4 universal primers of the Miseq sequencing platform: F primer 5'-ACTCCTACGGGAGGCAGCAG-3' and R primer: 5'-GGACTACHVGGGTWTCTAAT-3'; the library was diluted to a certain concentration according to the computer requirements, and sequenced with Miseq.

[0025] The test results showed that Alcaligenes (27%), Flavobacterium (29%), Lysobacterium (30%) and Pseudomonas (13%) were dominant bacteria in the root system of Canna, and diox...

Example Embodiment

[0026] Example 2

[0027] Take domestic sewage and mix it with clean water according to the volume ratio of 3:4 to cultivate ungerminated canna seeds as a culture solution. When the root length reaches about 10cm, rinse the roots of the canna so that the root microorganisms are dispersed in the culture solution. The OD of the culture solution 600nm =0.6; filter with 0.22μm filter membrane, take the filter membrane in DNA extraction kit ( In DNAIsolationKit), the extracted DNA product is first used for sample amplification. The PCR primers used the V3-V4 universal primers of the Miseq sequencing platform: F primer 5'-ACTCCTACGGGAGGCAGCAG-3' and R primer: 5'-GGACTACHVGGGTWTCTAAT-3'; the library was diluted to a certain concentration according to the computer requirements, and sequenced with Miseq.

[0028] The test results show that Alcaligenes (30%), Flavobacterium (25%), Lysobacterium (28%) and Pseudomonas (16%) are the dominant bacteria in the root system of Canna, and dioxygenase...

Example Embodiment

[0029] Example 3

[0030] Step 1. The domestic sewage is mixed with clean water according to the volume ratio of 1:2 to cultivate ungerminated canna seeds. When the root length reaches about 10cm, rinse the roots of the canna so that the root microorganisms are dispersed in the culture solution. The OD of the culture solution 600nm =0.4;

[0031] Step 2. OD 600nm =0.4 The culture solution is 1L / m 3 They are evenly mixed into soil and gravel to form a wetland matrix;

[0032] Step 3. Spread the gravel and soil in step 2 into the constructed wetland pool according to the volume ratio of 4:1, the soil is attached to the gravel, and the soil thickness is less than 1 meter; then it is 6 plants / m 2 The planting density is cross-planting two kinds of wetland plants; figure 2 As shown, the two cross-planted wetland plants are Juncus and Zailihua, Reeds and Windmill, Acorus and Lythrum, Canna and Shallots, Barracuda and Yellow Iris, Small Cattail and Dripping Guanyin.

[0033] Step 4. Use a pe...

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Abstract

The invention discloses an underflow constructed wetland ecological system for disposing rural domestic waste and a disposing method. By adding root advantageous microorganisms in a canna water planting system, ratio of Alkaligenes, flavobacterium, lysobacter, Pseudomonas and other advantageous germs can be rapidly increased, the abundance of dioxygenase, dehydrogenase, phosphate dehydrogenase, urease, nitrate reductase, and dehydrogenase gene in catalase gene in the constructed wetland can be improved; two kinds of wetland plants can be planted in an intersecting manner, thus the different plant root secreta can be generated and a rich root microbial community structure is formed; the underflow constructed wetland ecological system can remove COD, ammonia nitrogen and total phosphorus atthe same time. Then the method of alternatively running continuous water feeding-intermittent water feeding is applied, thus the nitrogen removal efficiency is promoted while the system is stably operated. The method fully applies the advantages of the constructed wetland, strengthens the rapid construction of the constructed wetland ecological system, and optimizes the wetland running mode; the underflow constructed wetland ecological system is significant in environment, economic and social values.

Description

technical field [0001] The invention relates to the technical field of environmental protection for rural domestic sewage treatment, in particular to an underflow artificial wetland ecosystem and a treatment method for treating rural domestic sewage that can operate stably and efficiently remove COD, ammonia nitrogen and total phosphorus at the same time. Background technique [0002] Rural domestic sewage has the characteristics of wide area and large volume, complex treatment conditions and weak foundation. Based on this, constructed wetlands can use the physical, chemical and biological triple coordination of the substrate-microbe-plant complex ecosystem to achieve efficient purification of rural domestic sewage; especially subsurface flow constructed wetlands (SFCWs), whose water flow is located below the surface of the filler , through the multiple functions of plant root system, surface biofilm and filler interception, it has a good pollutant removal effect, and this t...

Claims

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Application Information

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IPC IPC(8): C02F3/32C02F101/16C02F101/10
CPCC02F3/32C02F2101/105C02F2101/16C02F2203/006
Inventor 范海青王凌文殷操王丹俞河
Owner ZHEJIANG METALLURGICAL RES INST
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