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Underflow constructed wetland ecological system for disposing rural domestic waste and disposing method

A technology for constructed wetlands and domestic sewage, applied in biological treatment devices, chemical instruments and methods, biological water/sewage treatment, etc. The effect of faster builds, lower costs, optimized operation

Active Publication Date: 2018-02-13
ZHEJIANG METALLURGICAL RES INST +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the intermittent operation mode has unstable factors for the treatment of rural domestic sewage, and it is difficult to ensure the continuous operation of the system in large quantities

Method used

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  • Underflow constructed wetland ecological system for disposing rural domestic waste and disposing method
  • Underflow constructed wetland ecological system for disposing rural domestic waste and disposing method
  • Underflow constructed wetland ecological system for disposing rural domestic waste and disposing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take domestic sewage, mix it with clean water according to the volume ratio of 1:4, and cultivate ungerminated canna seeds as a culture medium. When the root length reaches about 10cm, rinse the canna roots to make the root microorganisms disperse in the culture medium, and the OD of the culture medium 600nm = 0.2; filter with a 0.22 μm filter membrane, take the filter membrane in the DNA extraction kit ( DNAIsolationKit), the extracted DNA product is first used for sample amplification. The PCR primers used the V3-V4 general primers of the Miseq sequencing platform: F primer 5'-ACTCCTACGGGAGGCAGCAG-3' and R primer: 5'-GGACTACHVGGGTWTCTAAT-3'; the library was diluted to a certain concentration according to the requirements of the machine, and sequenced by Miseq.

[0025] The test results showed that Alcaligenes (27%), Flavobacterium (29%), Lysobacterium (30%) and Pseudomonas (13%) were dominant bacteria in canna root system, and dioxygenase , dehydrogenase, phosphate ...

Embodiment 2

[0027] Take domestic sewage, mix it with clear water according to the volume ratio of 3:4, and cultivate ungerminated canna seeds as a culture medium. When the root length reaches about 10cm, rinse the canna roots so that the root microorganisms are dispersed in the culture medium, and the OD of the culture medium 600nm =0.6; filter with a 0.22 μm filter membrane, take the filter membrane in the DNA extraction kit ( DNAIsolationKit), the extracted DNA product is first used for sample amplification. The PCR primers used the V3-V4 general primers of the Miseq sequencing platform: F primer 5'-ACTCCTACGGGAGGCAGCAG-3' and R primer: 5'-GGACTACHVGGGTWTCTAAT-3'; the library was diluted to a certain concentration according to the requirements of the machine, and sequenced by Miseq.

[0028] The test results showed that Alcaligenes (30%), Flavobacterium (25%), Lysobacterium (28%) and Pseudomonas (16%) were dominant bacteria in canna root system, and dioxygenase , dehydrogenase, phosph...

Embodiment 3

[0030] Step 1. Domestic sewage is mixed with clean water at a volume ratio of 1:2 to cultivate ungerminated canna seeds. When the root length reaches about 10 cm, rinse the roots of canna to disperse the root microorganisms in the culture solution. The OD of the culture solution 600nm =0.4;

[0031] Step 2, the OD 600nm =0.4 culture medium according to 1L / m 3 Mixed evenly into the soil and gravel respectively to form the wetland matrix;

[0032] Step 3. Put the gravel and soil in step 2 into the artificial wetland pool according to the volume ratio of 4:1, the soil is attached to the gravel, and the soil thickness is less than 1 meter; then according to 6 plants / m 2 The planting density of two kinds of wetland plants is cross-planted; such as figure 2 As shown, the two kinds of wetland plants cross-planted are rushes and reeds, reeds and windmills, calamus and quinces, canna and water onions, pike grass and yellow iris, small cattails and dripping Guanyin.

[0033] Step 4...

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Abstract

The invention discloses an underflow constructed wetland ecological system for disposing rural domestic waste and a disposing method. By adding root advantageous microorganisms in a canna water planting system, ratio of Alkaligenes, flavobacterium, lysobacter, Pseudomonas and other advantageous germs can be rapidly increased, the abundance of dioxygenase, dehydrogenase, phosphate dehydrogenase, urease, nitrate reductase, and dehydrogenase gene in catalase gene in the constructed wetland can be improved; two kinds of wetland plants can be planted in an intersecting manner, thus the different plant root secreta can be generated and a rich root microbial community structure is formed; the underflow constructed wetland ecological system can remove COD, ammonia nitrogen and total phosphorus atthe same time. Then the method of alternatively running continuous water feeding-intermittent water feeding is applied, thus the nitrogen removal efficiency is promoted while the system is stably operated. The method fully applies the advantages of the constructed wetland, strengthens the rapid construction of the constructed wetland ecological system, and optimizes the wetland running mode; the underflow constructed wetland ecological system is significant in environment, economic and social values.

Description

technical field [0001] The invention relates to the technical field of environmental protection for rural domestic sewage treatment, in particular to an underflow artificial wetland ecosystem and a treatment method for treating rural domestic sewage that can operate stably and efficiently remove COD, ammonia nitrogen and total phosphorus at the same time. Background technique [0002] Rural domestic sewage has the characteristics of wide area and large volume, complex treatment conditions and weak foundation. Based on this, constructed wetlands can use the physical, chemical and biological triple coordination of the substrate-microbe-plant complex ecosystem to achieve efficient purification of rural domestic sewage; especially subsurface flow constructed wetlands (SFCWs), whose water flow is located below the surface of the filler , through the multiple functions of plant root system, surface biofilm and filler interception, it has a good pollutant removal effect, and this t...

Claims

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Application Information

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IPC IPC(8): C02F3/32C02F101/16C02F101/10
CPCC02F3/32C02F2101/105C02F2101/16C02F2203/006
Inventor 范海青王凌文殷操王丹俞河
Owner ZHEJIANG METALLURGICAL RES INST
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