Molecular diagnostic method for nitrogen nutrition of field potato plants and application of method in fertilization

Abstract

The invention discloses a molecular diagnostic method for nitrogen nutrition of field potato plants. Specifically speaking, a nitrate reductase (NR) gene related to nitrogen absorption by potato plants is used as a molecular marker for judging the states of nitrogen nutrition of field potato plants. During diagnosis, RNA of a field potato plant is used as a template, reverse transcription is carried out to obtain cDNA, the expression level of the NR gene is detected by using real-time PCR, the state of nitrogen nutrition of the field potato plant is judged according to the expression level of the NR gene, and whether to carry out topdressing of a nitrogenous fertilizer and the amount of topdressing of the nitrogenous fertilizer can be further determined. The diagnostic method has the advantages of good accuracy in judgment, high sensitivity, etc.

Description

technical field [0001] The invention belongs to the technical field of potato nutrition and fertilization management, and relates to a molecular diagnosis method for nitrogen nutrition of field potato plants. More specifically, the Real time PCR method is used to quantitatively analyze the expression of NR gene in plant leaves. To judge the nitrogen nutrition status of field potato plants, and determine whether fertilization and the specific amount of fertilization are needed, and provide guidance for field nitrogen management. Background technique [0002] Nitrogen is an indispensable nutrient element for crop growth and development. It is a component of chlorophyll, protein, and nucleic acid in plants, and a component of many endogenous hormones, accounting for 1% to 7% of plant dry weight. The level of chlorophyll content directly affects the size of photosynthesis, and reflects the level of nitrogen in leaves to a certain extent. Nitrogen fertilizer management plays an ...

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Problems solved by technology

Li et al. found that the gene expression of nitrate reductase and nitrite reductase in potato test tube seedlings was down-regulated within 3 days under the condition of nitrogen deficiency through hydroponic method, but under the condition of artificial greenhouse, the nutrient solution was used, the nitrogen was nitrate nitrogen, and the set nitrogen concentration Less gradient (sudden or large decrease in nitrogen concentration, sufficient (7.5 mM NO3-), limited (0.75 mM NO3-), and nitrogen deficiency (0 mM NO3-), the plants used are thin test-tube plantlets (plant height 8-10cm, stem diameter 2-3mm), only for a short period of time (7 days) cultivation, not involved in the formation of potato tubers, swelling, and not involved in the yield, the need for less nitrogen, while potato production is carried out in the field, using seed potato cutting Planted in blocks, the plants are strong (plant height 50-70cm, stem diameter 10-15mm), the soil itself contains a certain concentration of nitrogen, and the seed potatoes contain certain nutrients for the plants to absorb in the early stage of growth. The nitrogen used in the field is urea or compound Different from nitrate nitrogen in hydroponics, the fertilization level is not the molar concentration, but the fertilization amount per unit area (15kg pure N per mu is commonly used for potatoes in southern China), and the planting time is a long growing season , more than 80 days, including 5 periods of potato emergence, seedling, tuber formation, tuber expansion and tuber maturity, involving the formation of yield and requiring a lot of nitrogen, so potato plants in the field environment are far more than Hydroponic test-tube plantlets are complicated, and the research results of Li et al. have no reference significance for nitrogen nutrition diagnosis and fertilization of field potato plants

So far, there is no research report on using the expression of NR gene as a measure of the nitrogen nutrition status of field potato plants

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