11 results about "Molecular Diagnostic Method" patented technology

To provide a molecular diagnosis method using a composition for cytolysis for nucleic acid extraction.SOLUTION: The present invention relates to a molecular diagnosis method using a composition for cytolysis for nucleic acid extraction in which: by containing only Tween20 as a solution for extracting nucleic acid and applying a polymerase chain reaction to a mixture to which only a PCR buffer solution has been added for the polymerase chain reaction, the polymerase chain reaction can be performed without another nucleic acid extraction process to minimize the time for molecular diagnosis, and the cost of molecular diagnosis can be reduced by minimizing dedicated devices and consumables used for extraction.SELECTED DRAWING: Figure 2

The application provides a molecular diagnosis method combining a multi-enzyme isothermal rapid amplification technology (MIRA) and a CRISPR-Cas12a nucleic acid detection technology, and particularly relates to application thereof in rapid, sensitive and specific detection of common pathogenic bacteria of a central nervous system (CNS). The application combines the multi-enzyme isothermal rapid amplification technology and the CRISPR-Cas12a nucleic acid detection system, realizes rapid, sensitive and specific detection of nucleic acids of common pathogenic bacteria of the CNS under a constant temperature condition, and thus provides a new technical means for early diagnosis of the CNS infection.

The invention provides a molecular diagnosis method for avian leukosis virus and Marek's disease virus mixed infection. The molecular diagnosis method comprises the following steps: (S01) collecting diseased material tissues; (S02) carrying out nucleic acid extraction on the sick material tissue of the sick chicken; (S03) detecting the nucleic acid by using specific primers of avian leukosis and Marek's disease; (S04) preparing a PCR (Polymerase Chain Reaction) detection system, and respectively adding ALV-env gene amplification primers and MDV-meq gene amplification primers for gene amplification; (S05) carrying out 1% agarose gel electrophoresis detection on the amplification product in the step (4), and carrying out sequence determination after determining a target band; (S06) carrying out BLAST comparison on the sequences obtained by sequencing, carrying out gene sequence analysis and drawing an evolutionary tree; and (S07) determining the virus type according to the homology and the evolutionary branches, so as to realize rapid diagnosis of ALV and MDV mixed infection, strain characteristic analysis and exclusion of other similar epidemic diseases, and provide technical support for accurate prevention and control of AL and MD in the poultry industry, provenance purification and epidemiological investigation.

To provide a composition for cell lysis and nucleic acid extraction, and a nucleic acid extraction method using the same.SOLUTION: The present invention relates to a composition for cell lysis and nucleic acid extraction, a nucleic acid extraction method using the same, and a molecular diagnosis method using the same, which can minimize the time for molecular diagnosis by performing a polymerase chain reaction without a separate purification process and elution process by using a composition containing an RNase inhibitor as a solution for extracting nucleic acids and including a step of heating at a specific temperature, and can reduce the cost of molecular diagnosis by minimizing a dedicated device and consumables used for extraction.SELECTED DRAWING: Figure 2

The photothermal material-based lab-on-a-chip, the molecular diagnosis method using same, and the molecular diagnosis apparatus using same according to the present invention enable highly efficient PCR to be performed within a short period of time without concerns regarding substrate effects. In addition, analyses of different targets can be carried out simultaneously, thereby providing an advantage of enabling multiplex diagnosis. Therefore, the photothermal material-based lab-on-a-chip, the molecular diagnosis method using same, and the molecular diagnosis apparatus using same according to the present invention can be used as point-of-care diagnostic tools requiring rapidity, accuracy, and multiplex diagnostic capability.

This invention discloses a strategy for rapid detection of isoniazid resistance in Mycobacterium tuberculosis based on multiplex polymerase chain reaction (Multiplex PCR) using local rTth DNA polymerase and interpretation of amplification patterns. This strategy utilizes a specific primer combination that allows simultaneous amplification of the control fragment and the target fragment of the katG gene codon 315 mutation in a single PCR reaction. The amplification products are analyzed by 1% (w / v) agarose gel electrophoresis and interpreted based on the resulting DNA banding pattern to distinguish sensitive isolates, isolates carrying the katG315 mutation causing isoniazid resistance, and invalid test results. The use of local rTth DNA polymerase provides an alternative thermostable enzyme to support national diagnostic raw material independence without changing the detection principle.This strategy allows for rapid identification of katG315 mutations associated with isoniazid resistance without the need for DNA sequencing or further molecular analysis. The invention provides a simple, rapid, specific, easily interpretable, and cost-effective molecular diagnostic method to support the early detection of isoniazid-resistant tuberculosis.

Presented herein are techniques for qualifying, checking and monitoring the use of diagnostic apparatuses and reagents, such as sequencing apparatuses and reagents, to ensure performance and quality of diagnostic methods. In one aspect, a system is provided for determining whether a sequencing run is a qualification run. The system includes a memory configured to store identifiers of sequencing apparatuses and an associated status, a network interface configured to communicate with a sequencing apparatus and a client terminal, and a processor configured to receive a report from the sequencing apparatus via the network interface for a sequencing run, and determine whether the sequencing run is designated as a qualification run.

The invention discloses a single cross primer pair for a rahnella aquatica DNA target sequence OmpA gene and application of an in-vitro isothermal amplification rapid detection method of the single cross primer pair. Nucleotide sequences of the specific DNA target OmpA gene primer pair for detecting the rahnella aquatica are shown as SEQ ID NO.1-5. The invention develops a rapid detection and molecular diagnosis method for Rahnella aquatica under a 62-65 DEG C isothermal amplification condition on the basis of establishing a Rahnella aquatica OmpA gene single cross primer sequence and an isothermal amplification rapid detection technical method thereof. According to the method, five specific primer sequences of a Rahnella aquatica OmpA target gene are used for identifying a specific DNA domain of the Rahnella aquatica OmpA target gene, amplification detection is completed within 60 minutes, the method has high specificity and high sensitivity to Rahnella aquatica, and the lowest detection limit is 48 pg / mL. Through the molecular diagnosis method provided by the invention, the rahnella aquatica detection method is simpler, more convenient and quicker, and the clinical diagnosis is more accurate; the invention provides an efficient and rapid technical means for Rahnella aquatic OmpA gene target sequence detection or DNA target molecular diagnosis, and has important application value for quarantine, molecular diagnosis and epidemiological monitoring of diseases caused by Rahnella aquatic in clinical practice.

Presented herein are techniques for qualifying, checking and monitoring the use of diagnostic apparatuses and reagents, such as sequencing apparatuses and reagents, to ensure performance and quality of diagnostic methods. In one aspect, a system is provided for determining whether a sequencing run is a qualification run. The system includes a memory configured to store identifiers of sequencing apparatuses and an associated status, a network interface configured to communicate with a sequencing apparatus and a client terminal, and a processor configured to receive a report from the sequencing apparatus via the network interface for a sequencing run, and determine whether the sequencing run is designated as a qualification run.

The invention discloses an application of an HLA (human leukocyte antigen) gene SNP (single nucleotide polymorphism) site in predicting the genetic susceptibility risk of immune thrombocytopenic purpura of Chinese population, and the HLA gene SNP site is rs2187668 and has Cgt; according to the present invention, the genotype of the site is detected, and the genotypes of the site have different risks of immune thrombocytopenic purpura, such that the effective prediction can be performed by detecting the genotype of the site so as to achieve the purpose of predicting the Chinese iTTP genetic susceptible population through the molecular diagnosis method detection, and the significant clinical diagnosis application value is provided.

According to the method, gene expression specific to the cell tumor state in a predefined gene list is analyzed by using a trained binary classifier algorithm, so that the tumor of a biological sample can be estimated.