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SNP markers related to assessment of incidence risks of NK/T cell lymphoma and application of SNP markers

A lymphoma and marker technology, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial measurement/inspection, etc., can solve the problems that the susceptibility of the population has not been verified, and the risk of NK/T cell lymphoma is low , to achieve the effects of convenient material collection, increased sensitivity, and improved applicability

Pending Publication Date: 2020-06-19
SUN YAT SEN UNIV CANCER CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the pathogenesis of NK / T-cell lymphoma is complex and affected by environmental and genetic factors, and the risk of NK / T-cell lymphoma assessed by a single SNP is low (OR<2)
In addition, the susceptibility of people in areas with a high incidence of NK / T-cell lymphoma outside Asia has not yet been verified

Method used

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  • SNP markers related to assessment of incidence risks of NK/T cell lymphoma and application of SNP markers
  • SNP markers related to assessment of incidence risks of NK/T cell lymphoma and application of SNP markers
  • SNP markers related to assessment of incidence risks of NK/T cell lymphoma and application of SNP markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1. Extract the DNA from the sample:

[0038] Take 2ml of whole blood, and use the kit QIAamp DNA Mini Kit Cat.No.51304 to extract DNA. The specific steps are as follows:

[0039] (1) Add 200ul QIAGEN protease to a 15ml centrifuge tube, add 2ml blood sample, shake and mix;

[0040] (2) Add 2.4ml of AL buffer solution, shake and mix well, and incubate in a water bath at 70°C for 10 minutes;

[0041] (3) Add 2ml of absolute ethanol, mix upside down;

[0042] (4) Take half of the volume of the above liquid and add it to a 15ml QIAGEN separation column, centrifuge at 3000g for 3 minutes, and discard the filtrate;

[0043] (5) Repeat (5) for the remaining liquid in (3);

[0044](6) Add 2ml AW1 to the separation column, centrifuge at 3000g for 1 minute, and discard the filtrate;

[0045] (7) Add 2ml AW2 to the separation column, centrifuge at 3000g for 1 minute, and discard the filtrate;

[0046] (8) Centrifuge the separation column at 3000g for 1-2 minutes, and prepare a...

Embodiment 2

[0123] The preparation method of embodiment 2 prediction kit

[0124] (1) Using the above method to extract the genomic DNA from the peripheral blood of the subject to be tested.

[0125] (2) Use specific primers to amplify the fragments containing the three sites of rs13015714, rs9271588, and rs9277378. The following is the primer information as shown in Table 2:

[0126] Table 2: Amplification Primers

[0127]

[0128] (3) PCR reaction system and conditions:

[0129] The reaction system is shown in Table 3 below:

[0130] Table 3: Reaction system

[0131] Reagent Usage amount Final concentration 5xPCR buffer 2μl dNTP Mixture (2.5mM) 2μl 200uM magnesium chloride 1μl forward primer 6pmol 0.5uM reverse primer 6pmol 0.5uM Genomic DNA in the peripheral blood of the test subject 100-200ng DNA polymerase 1μl 1U / 20μl Deionized water Add to 20μl

[0132] Reaction Condition Table 4:

[01...

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Abstract

The invention provides a set of SNP markers related to assessment of the incidence risks of NK / T cell lymphoma. The SNP markers comprise rs13015714, rs9271588 and rs9277378 sites. Sampling of testersis convenient, only peripheral blood is needed, no other tissue samples are needed, SNP is detected through simplified and specific amplification primers so that the operation is simple, and then theincidence risks of individuals are assessed through SNP typing. High-risk groups of NK / T cell lymphoma can be confirmed more accurately through a molecular diagnosis method so that the groups are guided to take preventive intervention measures, and the applicability and sensitivity are increased at the same time, so that the SNP markers are further applied to scientific research and clinical work.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and specifically relates to a group of SNP markers related to evaluating the risk of NK / T cell lymphoma and its application. Background technique [0002] NK / T cell lymphoma (Extranodal natural killer / T cell lymphoma, NKTCL) is a relatively rare non-Hodgkin lymphoma derived from NK cells or T cells. The incidence of the disease is very low in western Caucasian population, relatively high in Asia and South America, and the incidence in East Asia is 0.13-0.25 / 100,000, suggesting that the incidence of NK / T cell lymphoma may be affected by susceptibility genes . Most NK / T cell lymphomas occur in the nasal cavity and paranasal sinuses. The clinical manifestations are strong invasion, insensitive to chemotherapy, easy to develop drug resistance, short survival period, poor prognosis, and the 5-year survival rate in the advanced stage is less than 50% %. The early symptoms and signs of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/156
Inventor 贝锦新林国旺彭柔君陈杰荣
Owner SUN YAT SEN UNIV CANCER CENT
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