A molecular diagnosis method of nitrogen nutrition in field potato plants and its application in fertilization

Abstract

The invention discloses a nitrogen nutrition molecular diagnosis method for field potato plants. Specifically, the nitrogen absorption related gene nitrate reductase gene (NR) of potato plants is used as a molecular marker for measuring the nitrogen nutrition status of field potato plants. During the diagnosis, the RNA of the field potato plant was used as the template, the cDNA was obtained by reverse transcription, and the Real? time? The expression level of NR gene was detected by PCR. According to the expression level of NR gene, the nitrogen nutrition status of field potato plants could be judged, and then whether topdressing nitrogen fertilizer and its application rate could be determined. The diagnostic method has the advantages of good judgment accuracy and high sensitivity.

Description

technical field [0001] The invention belongs to the technical field of potato nutrition and fertilization management, and relates to a molecular diagnosis method for nitrogen nutrition of field potato plants. More specifically, the method of Realtime PCR is used to quantitatively analyze the expression of NR gene in leaves of plants to judge The nitrogen nutrient status of field potato plants, and determine whether fertilization and the specific amount of fertilization are required, and provide guidance for field nitrogen management. Background technique [0002] Nitrogen is an indispensable nutrient element for crop growth and development. It is a component of chlorophyll, protein, and nucleic acid in plants, and a component of many endogenous hormones, accounting for 1% to 7% of plant dry weight. The level of chlorophyll content directly affects the size of photosynthesis, and reflects the level of nitrogen in leaves to a certain extent. Nitrogen fertilizer management pla...

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Problems solved by technology

Li et al. found that the gene expression of nitrate reductase and nitrite reductase in potato test tube seedlings was down-regulated within 3 days under the condition of nitrogen deficiency through hydroponic method, but under the condition of artificial greenhouse, the nutrient solution was used, the nitrogen was nitrate nitrogen, and the set nitrogen concentration Less gradient (sudden or large decrease in nitrogen concentration, sufficient (7.5mMNO 3 - ), limited (0.75mMNO 3 - ), and nitrogen deficiency (0mMNO 3 - ), the plants used are thin test-tube plantlets (plant height 8-10cm, stem diameter 2-3mm), only for a short period of time (7 days) for cultivation, not involving the formation and expansion of potato tubers, let alone yield, It needs less nitrogen, and potato production is carried out in the field. The seed potatoes are cut into pieces and planted. The plants are strong (plant height 50-70cm, stem diameter 10-15mm), and the soil itself contains a certain concentration of nitrogen. Seed potatoes It contains a certain amount of nutrients for the plants to absorb in the early stage of growth. The nitrogen applied in the field is urea or compound fertilizer, which is different from the nitrate nitrogen used in hydroponics. The fertilization level is not the molar concentration, but the fertilization amount per unit area (generally used for potatoes in southern China. The planting time is a long growing season, more than 80 days, including five periods of potato emergence, seedling, tuber formation, tuber expansion and tuber maturity, involving A large amount of nitrogen is needed for the formation of yield, so potato plants in the field environment are far more complicated than hydroponic test-tube seedlings. The research results of Li et al. have no reference significance for the nitrogen nutrition diagnosis and fertilization of potato plants in the field.

So far, there is no research report on using the expression of NR gene as a measure of the nitrogen nutrition status of field potato plants

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