A norfloxacin-degrading Acinetobacter calcoacetate nor‑36 and its application
A technology of NOR-36 and norfloxacin, which is applied in norfloxacin to degrade Acinetobacter calcoaceticus NOR-36 and its application field, can solve the problems affecting the development of animal husbandry and aquaculture industry, the impact of environmental microecology, etc. Achieve the effect of improving the quality and safety of the water environment, good removal effect, and protecting the ecological environment
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0025] Example 1 Norfloxacin degrades the screening and isolation of Acinetobacter calcoacetate NOR-36
[0026] Get the activated sludge 3.0ml that obtains from the wastewater treatment tank of a certain pesticide factory production workshop that produces norfloxacin and add 100ml norfloxacin concentration and be in the LB medium containing piperazine of 50mg / L, its formula is: Contain 5g / L piperazine, NaCl10.00g / L, peptone 10.00g / L, yeast powder 5.00g / L, pH 7.0, shake culture at 160r / min, 30℃, inoculate with 3% every 7 days The amount was transferred to fresh inorganic salt medium, and transferred 5 times in a row.
[0027] Take 1.0ml of the enriched bacteria solution obtained above, add 9.0ml of sterile water, and make 10 -1 The rich solution, and then draw 1.0ml prepared 10 -1 The enrichment solution was added to 9.0ml sterile water, mixed thoroughly to make 10 -2 The enrichment solution, and so on, carry out gradient dilution on the enrichment solution. The dilution so...
Embodiment 2
[0029] Example 2 Shake Flask Degradation Experiment of Acinetobacter Calcoacetate NOR-36
[0030] 2.1 The effect of inoculum size on the degradation efficiency of NOR-36
[0031]In the inorganic salt medium containing 500ug / L norfloxacin, the formula is: every liter contains 500ug / L piperazine, 1.50 gK 2 HPO 4 , 0.50g KH 2 PO 4 , 0.20g MgSO 4 ×7H 2 O, 1.00g NaCl, 1.00g (NH 4 ) 2 SO 4 , pH 7.0, inoculate NOR-36 in the culture medium according to the inoculum amount of 1%, 2%, 3%, 4%, 5%, shake culture at 30°C, and take samples for measurement after 72 hours. The result is given by figure 2 It can be seen that when 1% is inoculated, the degradation rate is 91%. With the increase of the inoculum amount, the degradation rate of norfloxacin increases slightly. Considering the reasons of bacterium growth and economy, the inoculum amount of 1% is adopted The requirement can be met.
[0032] 2.2 The effect of pH on the degradation efficiency of NOR-36
[0033] The pH valu...
Embodiment 3
[0035] Embodiment 3 Containing the preparation of calcium acetate Acinetobacter NOR-36 bacterial agent
[0036] The original species of norfloxacin-degrading calcoacetic acid Acinetobacter NOR-36 screened and isolated in Example 1 was activated on a petri dish, and its degradation performance was measured, and it was inoculated on the inclined surface of the test tube for subsequent use. The test tube was inoculated in a 1000ml shake flask containing 200ml of LB medium containing piperazine. The formula of LB medium was: piperazine 5g / L, yeast extract 5.00g / L, peptone 10.00g / L, NaCl10.00g / L, pH 7.0, constant temperature shaking culture to logarithmic phase, ready to inoculate seed tanks. The seed tank is 500 liters, the feeding amount is 400 liters, the medium formula is: piperazine 5g / L, glucose 8g / L, (NH 4 ) 2 SO 4 1g / L, K 2 HPO 4 2g / L, MgSO 4 0.5g / L, NaCl1g / L%, CaCO 3 0.5g / L, yeast extract 2g / L, pH 7.2-7.5. After the feeding is completed, 121°C high-pressure dam...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com