Rice nitrate reductase NIA3 protein gene OsNia3 and application thereof

Abstract

The invention discloses a rice nitrate reductase NIA3 protein gene OsNia3. The rice nitrate reductase NIA3 protein gene OsNia3 has a cDNA sequence shown as SEQ ID NO. 1 which encodes a rice NIA3 protein having an amino acid sequence shown as SEQ ID NO. 2. Homozygous mutants with rice gene OsNia3 knocked-out and homozygous stains with OsNia3 overexpression are obtained by utilizing a transgenic technology. It has been discovered by analysis that the stains with the OsNia3 gene knocked-out have shortened plant height, shorted growth period and reduced yield per plant; however, the stains with OsNia3 overexpression have relatively high plant height, prolonged growth period and increased yield per plant. Thus, the rice nitrate reductase NIA3 protein gene OsNia3 can be introduced into plants asa target gene, thereby improving nitrogen use efficiency of the plants so as to be subjected to plant variety improvement.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to a rice nitrate reductase NIA3 protein gene OsNia3 and an application thereof. Background technique [0002] Plants absorb nitrogen from soil primarily in the form of nitrate and ammonium, and nitrate assimilation is a highly regulated nitrogen utilization process [1] . The first step of nitrate degradation occurs in the cytoplasm. Nitrate is reduced to nitrite by nitrate reductase (NR). After nitrite enters the chloroplast or plastid, it is absorbed by the plant through a series of subsequent metabolic reactions. used. The current study found that there are two nitrate reductase genes (AtNia1 and AtNia2) in Arabidopsis, which encode NIA1 and NIA2 proteins, respectively. [2 ]. NR activity was significantly reduced in Arabidopsis mutants after knockout of AtNia1 and AtNia2, whereas almost no NR activity was detected in the nia1nia2 double knockout mutant [3] , over...

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Problems solved by technology

NR activity in Arabidopsis is mainly controlled by gene AtNia2, mutation of AtNia2 gene will lead to loss of 90% NR activity [5]

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