An improved compound microbial inoculant and its application in increasing the yield of Angelica sinensis

A technology of compound microbial agent and microbial fertilizer, applied in the direction of microorganism-based methods, microorganisms, chemicals for biological control, etc., can solve problems such as urea burning seedlings and ammonium salt poisoning that have not been considered, and promote the absorption of rich Accumulate, promote the conversion into ammonium nitrogen, enhance the effect of activity

Active Publication Date: 2022-07-19
甘肃绿能农业科技股份有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The inventor has studied the impact of Pseudomonas compound microbial bacterial agent on the physiology, biochemistry and quality of continuous cropping Angelica sinensis, and has applied for a national invention patent (a kind of Pseudomonas compound microbial bacterial agent and application in Angelica sinensis disease resistance, yield increase and quality improvement), but the problem of burning seedlings and ammonium salt poisoning caused by a large amount of urea and unreasonable use of farmers in the planting process has not been considered. Phosphorus can promote flowering. Therefore, Angelica planting related bacterial agents The development should also consider the problem of phosphorus solution

Method used

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  • An improved compound microbial inoculant and its application in increasing the yield of Angelica sinensis
  • An improved compound microbial inoculant and its application in increasing the yield of Angelica sinensis
  • An improved compound microbial inoculant and its application in increasing the yield of Angelica sinensis

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Experimental program
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Effect test

Embodiment 1

[0043] Embodiment 1 Determination of microbial phosphorus solution, nitrogen fixation and nitrification

[0044] 1. Qualitative detection of phosphorus dissolving ability

[0045] Different strains were inoculated on NBRIP inorganic phosphorus solid medium, and cultured at a constant temperature of 28 °C for 2 to 5 days. effect.

[0046] 2. Quantitative detection of nitrogen fixation activity

[0047] Take 5ml of purified bacterial liquid and inoculate it into sterilized 50ml of Axubi nitrogen-free medium, 200r·min -1 , 30 ℃ shaking culture for 72h, measure the turbidity OD 600 .

[0048] Determination of nitrogenase activity of the strain by acetylene reduction method: transfer 5ml of the above fresh bacterial liquid into a 20ml headspace bottle and seal it, extract 2ml of gas from the bottle, inject an equal volume of acetylene, continue to cultivate for 24h, use a gas chromatograph The amount of ethylene produced was detected. Gas chromatographic analysis conditions: ...

Embodiment 2

[0058] The influence of embodiment 2 different treatments on the yield-related indexes of Angelica sinensis

[0059] After harvesting, the number of adventitious roots was calculated by conventional methods, the diameter, head length and body length of Angelica were measured with vernier calipers, and the fresh weight of each plant was weighed by electronic scales.

[0060] The results are shown in Table 2. Compared with CK, the adventitious root number, reed head diameter, reed head length, body length and average fresh weight of angelica were basically unchanged after treatment with CBS4 single bacteria. The number of adventitious roots, diameter of reed head, reed head length, body length and average fresh weight per plant all increased. Compared with the CK group, the number of adventitious roots, the diameter of the head, the length of the head, the length of the head, and the average fresh weight per plant increased by 14.30% and 19.74%, respectively, after being treated...

Embodiment 3

[0064] Embodiment 3 Angelica rhizosphere soil enzyme activity assay

[0065] Determination of rhizosphere soil urease (S-UE), soil sucrase (S-SC), soil FDA hydrolase, soil β-glucosidase (S-β-GC), soil alkalinity at different stages of Angelica sinensis according to the method described in the kit Protease (S-ALPT), soil alkaline phosphatase activity (S-AKP / ALP), soil catalase (S-CAT) activity, each sample was repeated three times, and the average value was calculated.

[0066]The effects of different treatments on rhizosphere soil enzyme activities are shown in Table 3. Compared with the CK group, the treatment of T1 and T2 compound inoculants (S2 and S3 periods) all increased the levels of FDA hydrolase, soil invertase, soil-β-glucosidase, soil urease, soil alkaline protease in the rhizosphere soil of Angelica sinensis. The results showed that the two compound inoculants could improve the microbial activity of the rhizosphere soil and the transformation ability of organic ma...

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Abstract

The invention belongs to the field of microorganisms and biotechnology, and in particular relates to an improved compound microorganism inoculum and its application in increasing the yield of Angelica sinensis. The composite microbial inoculum is compounded by Pseudomonas fluorescens, Pseudomonas alcaligenes, Pseudomonas psychrophilic and Zygomycota; Functional characteristics, and improve the transformation, absorption and utilization of soil nutrients; and the compound microbial inoculum can not only promote the conversion of ammonium nitrogen in the rhizosphere soil to nitrate nitrogen, reduce the toxic effect of soil ammonium salt on plants, and improve It can also enhance the activity of nitrate reductase in plants, promote the conversion of nitrate nitrogen in plants to ammonium nitrogen, promote the absorption and enrichment of nitrogen by plants, and improve plant yield.

Description

technical field [0001] The invention belongs to the field of microorganisms and biotechnology, and in particular relates to an improved compound microorganism inoculum and its application in increasing the yield of Angelica sinensis. Background technique [0002] Angelica is the dried root of Angelica sinensis (Oliv.) Diels of the Umbelliferae plant. It is a traditional bulk Chinese medicine and has been included in the catalogue of medicinal and food homology. Gansu is an Angelica Dao production area, with a planting area of ​​400km in the province 2 , the output is 1.2×10 5 kg, accounting for more than 80% of the total supply in the national market, and it plays a decisive role in the market price of angelica. Angelica sinensis can be cultivated at an altitude of about 1500-3000m. It likes alpine and cool climate. It likes deep, loose, well-drained, fertile sandy loam rich in humus. It is not suitable for low-lying stagnant water or easily compacted clay and barren sand ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/27A01N63/20A01P21/00C05G3/90C05G3/00C12R1/39C12R1/38C12R1/01
CPCC12N1/20A01N63/27A01N63/20C05G3/90C05G3/00Y02P60/21
Inventor 杨涛牛永艳王治业赵疆毛婷姚阳阳李鑫
Owner 甘肃绿能农业科技股份有限公司
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