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Method for inhibiting generation of nitrite ion or nitrite in cubilose

A technology of nitrite and nitrite, applied in the field of inhibiting the production of nitrite or nitrite in bird's nest, can solve the problems of lack of data support, loss percentage, etc.

Active Publication Date: 2014-05-28
INFINITY SGC HK LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the percentage of nutrients lost in bird's nest during repeated washing and reshaping, and the amount of nitrite and nitrite remaining in bird's nest after treatment are not supported by data

Method used

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  • Method for inhibiting generation of nitrite ion or nitrite in cubilose
  • Method for inhibiting generation of nitrite ion or nitrite in cubilose
  • Method for inhibiting generation of nitrite ion or nitrite in cubilose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Embodiment 1. Contain a large amount of nitrate or nitrate in the natural environment of bird's nest and its place of production

[0049] According to the method described in China's "National Food Safety Standard-Determination of Nitrite in Food" GB5009.33-2010, various types of bird's nests sold in the market and naturally harvested were tested. The inventor has detected the content of nitrate radicals in the bird's nests that are harvested from commercially available and natural places of origin, wherein commercially available Cave Bird's Nest, House Red Bird's Nest and House Yellow Bird's Nest are from places such as Indonesia, Vietnam, Malaysia and Thailand respectively (see Table 1), Cave swallows, house red swallows and house yellow swallows harvested from natural origins are respectively harvested from Gomantong Cave in Malaysia and Swiftlet House on Java Island in Indonesia. As a result, it was found that no matter commercially available or naturally harvested ...

Embodiment 2

[0058] Embodiment 2. Nitrate reductase is contained in bird's nest

[0059] The inventor separated a specific protein band of about 43kDa from the red swallow in Sabah, Malaysia and the red swallow in Java, Indonesia, by SDS-PAGE and Coomassie blue staining technology (see image 3 ). The above-mentioned specific protein bands were decolorized, trypsinized and desalted, and then the protein peptide extract was analyzed by liquid phase time-of-flight mass spectrometer. Deduce the amino acid sequence of described protein belt according to protein peptide mass difference, this amino acid sequence comprises fragment APLLGCGCGNKGCMDNYLSGR (see Figure 4 ). After comparing with the protein sequence in the protein database SWISSPROT, it was confirmed that the specific protein band was the periplasmic nitrate reductase from Bradyrhizobium. The specific methods and steps are described in detail below.

[0060] SDS-PAGE analysis

[0061] For each edible bird's nest (EBN) (Malaysian...

Embodiment 3

[0068] Example 3. Nitrate or nitrate reduction in different types of bird's nests and the inhibitory effect of sodium tungstate on the production of nitrite or nitrite

[0069] Weigh 10mg of sodium tungstate (CAS#: 10213-10-2; SIGMA-ALDRICH) into a 100mL volumetric flask, add 80ml of pure water, and then sonicate for 5 minutes. Sodium tungstate aqueous solution.

[0070] The three kinds of bird's nests coated with the above-mentioned sodium tungstate inhibitor (namely, Indonesian house white bird's nest, Indonesian house yellow bird's nest and Indonesian house red bird's nest) and the control bird's nest without sodium tungstate inhibitor were soaked in a solution containing 1M potassium nitrate or pure water in a test tube and placed in a 37°C water bath for 15 days. After 15 days, the nitrite or nitrite content in all test tubes was measured according to the method described in the Chinese "National Standard for Food Safety-Determination of Nitrite in Foodstuffs" GB5009.33-...

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Abstract

The invention relates to a method for inhibiting generation of nitrite ion or nitrite in health care products, food or medicinal material containing nitrate ion or nitrate and nitrate reductase. The method comprises inhibiting the activity of nitrate reductase in the health care products, food or medicinal material by using the health care food, food or medically accepted nitrate reductase. The invention particularly relates to a method for inhibiting generation of nitrite ion or nitrite in cubilose, comprising using cubilose-accepted nitrate reductase inhibitor to inhibit the activity of the nitrate reductase in cubilose. Particularly, the method comprises adding the cubilose-accepted nitrate reductase inhibitor with effective inhibiting amount in newly collected cubilose or adding cubilose-accepted nitrate reductase inhibitor with the effective inhibiting amount in the cubilose production process. The nitrate reductase inhibitor can be selected from tungstate. By adopting the method, generation of nitrite ion or nitrite in cubilose can be effectively inhibited, and the edible risk of the cubilose can be lowered.

Description

technical field [0001] The present invention generally relates to a method for inhibiting the production of nitrite or nitrite in health products, food or medicinal materials containing nitrate or nitrate and nitrate reductase by using a nitrate reductase inhibitor, in particular to inhibiting A method for producing nitrite or nitrite in bird's nest, and a use of a nitrate reductase inhibitor for inhibiting the production of nitrite or nitrite in bird's nest. Background technique [0002] Nitrite belongs to highly toxic chemicals, and there are many related research reports explaining its pathological symptoms. Among them, due to immature digestive system and methaemoglobin reductase system, infants are more likely to suffer from methaemoglobinemia caused by nitrite, and the consequences can be fatal. In addition, nitrite and nitrate can react with stomach acid to produce compounds called nitrosamines. Nitrosamines are highly carcinogenic substances. It has been reported t...

Claims

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Application Information

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IPC IPC(8): A23L1/015A23L1/29A23L5/20A23L33/00
CPCA23L5/27A23L33/00
Inventor 詹华强董婷霞陈嘉伦
Owner INFINITY SGC HK LTD
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