Novel tuckahoe strain and efficient cultivation technology thereof

The technology of Poria cocos and strains is applied in the field of new strain breeding of Poria cocos and its high-efficiency cultivation, which can solve the problems of declining biological efficiency, backward cultivation technology, consumption of large pine resources, etc. The effect of strong anti-bacterial infection ability and improving the survival rate of inoculation

CN102742453AActive Publication Date: 2012-10-24HUNAN BUTIAN PHARMA
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2012-10-24

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Abstract

The invention discloses a novel tuckahoe strain (china center for type culture collection (CCTCC) M2011072) and bag-material efficient cultivation technology. The novel tuckahoe stain is applied to cultivation by adopting the bag-material efficient cultivation technology. The novel tuckahoe stain is mainly characterized in that 1) a mycelium is strong, white, obvious in clamp connection; and hypha is spread quickly in a cultivation process; 2) the tuckahoe fruits earlier, the fruit body is even and strong, the color of pulp is white or light yellow, and the skin of tuckahoe is thin and presents purplish red color; 3)the novel tuckahoe strain has the advantages of being stable inheritable characteristics, strong in anti-bacteria infection capacity, high in biological efficiency and yield, good in quality and the like; and 4) the novel tuckahoe strain remarkably improves content of effective components of amino acid, pachymaran and the like in the tuckahoe.
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Description

technical field

[0001] The invention relates to strain selection and cultivation technology thereof, in particular to new strain selection and high-efficiency cultivation technology of poria cocos. Background technique

[0002] Poria Cocos (schw) WOLF) is a polypore fungus (Basidiomycetes), sweet, light, and flat in nature. Traditional Chinese medicine is also one of the prescriptions of "Four Gentlemen and Eight Treasures", and its market prospect is very broad.

[0003] The main component of Poria cocos is β-pachyan (also known as "Poria cocos polysaccharide"), which accounts for about 93% of the dry weight. Poria polysaccharide is an immune enhancer, which has the functions of enhancing immunity, adjuvant chemotherapy, preventing and treating tumors, and anti-tumor. It has been widely used as a new anti-cancer drug, hepatitis rehabilitation drug, and used to treat bronchial asthma and chronic bronchitis. Existing Poria health products include Poria flat tablets, Poria r...

Examples

Embodiment 1

[0049] Example 1 Breeding Method and Character Analysis of Poria Cocos New Strain (CCTCC NO: M2011072)

[0050] The breeding method of Poria cocos new strain (CCTCC NO: M2011072) of the present invention comprises the steps:

[0051] 1) Cultivation of mycelium: select wild Poria cocos with uniform and firm body, thin skin and good quality, and carry out tissue culture at 24-28°C to separate sclerotia and mycelium, wherein the tissue cultured The medium consists of 1000 ml of water, 200 grams of potatoes, 20 grams of glucose, 20 grams of agar, 3 grams of potassium dihydrogen phosphate, 1.5 grams of magnesium sulfate, 1.5 grams of vitamin B1 and 5 grams of peptone;

[0052] 2) Purification and rejuvenation of mycelium: Inoculate the separated mycelium into multi-tube culture medium, then place it in a sterile box, and cultivate it at 26-28°C for 7-10 days until the mycelium is fully grown. Select the one with the best growth condition to carry out transplantation culture, wh...

Embodiment 2

[0056] Example 2 Study on the Efficiency of Poria Cocos New Strain (CCTCC NO: M2011072)

[0057] Promoted 523,000 packs of Poria cocos new strains (CCTCC NO: M2011072), cultivated 11.5 million bags with bags, created a total output value of 120.6086 million yuan, increased profits and taxes of 79.731 million yuan, earned foreign exchange of 192,200 US dollars from exports, and a total output value of 116 million yuan. Produced good ecological benefits, economic benefits and social benefits, the results are shown in Table 1.

[0058] Table 1

[0059]

[0060]

[0061] 1. Economic benefits

[0062] Promoting 523,000 packs of Poria cocos strains (CCTCC NO: M2011072) of the present invention, its sales income can reach 1.046 million yuan, deducting costs of 627,600 yuan, and realizing profits and taxes of 418,400 yuan; among them, 523,000 packs of strains can plant 221,900 bags (cellars) Poria cocos, with 15kg of raw materials per bag (cellar) and 50.6% biological effi...

Embodiment 3

[0071] Example 3 Comparative study on high-efficiency bag material cultivation and section wood cultivation of a new strain of Poria cocos (CCTCC NO: M2011072)

[0072] The new strain of Poria cocos (CCTCC NO: M2011072) is cultivated in 100 bags, with an average load of 5kg per bag, a total of 500kg; the new strain of Poria cocos (CCTCC NO: M2011072) is cultivated in 22 sections, a total of 488.5kg; Respective cultivation techniques require cultivation management.

[0073] Comparative study of the research results of the bag material cultivation and section tree cultivation of the new strain of Poria cocos (CCTCC NO: M2011072), the research results include the beginning period, the first batch of Poria cocos mature period, the mature period of Poria cocos, the harvested fresh Poria cocos The minimum mass and the maximum mass of the number, single fresh Poria cocos, the total weight and physical and chemical cost of the fresh Poria cocos harvested, etc., the results are sho...