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Miniature lotus root seed breeding technique for lotus root

A technology of miniature lotus root and lotus root is applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of inconvenient transportation, slow reproduction, large volume, etc., and achieves simple and easy cultivation technology, convenient transportation, Small size effect

Active Publication Date: 2012-06-20
WUHAN VEGETABLE RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It overcomes the shortcomings of conventional lotus root, such as slow propagation speed, large volume, and inconvenient transportation, and makes lotus root seedlings miniaturized, which is convenient for farmers to plant and produce in the field while being convenient for transportation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take Elian No. 5 test-tube lotus root and cultivate it in a circular container with a diameter of 30 cm and a height of 12 cm. After growth and development, the lotus root is multiplied to form a miniature seed lotus root. The specific steps are:

[0025] Step 1: Place the container and fill the substrate: clean up the weeds in the field, level the field, place the container, and fill the container with soil (mud). The diameter of the container is 30cm, the height is 12cm, and the soil (mud) is filled to 2 / 3 of the depth of the container.

[0026] Step 2: Planting: The daily average temperature is stable above 13°C, and the test tube lotus root of lotus root Elian No. 5 is transplanted into the prepared container.

[0027] Step 3: Add water or nutrient solution: Add 1cm of water to the container to make the plants grow normally.

[0028] Step 4: Fine management: During the growth and development of the miniature lotus root, water depth adjustment, top dressing, weed r...

Embodiment 2

[0034] Get the terminal buds of Elian No. 5 lotus root, cultivate them in a circular container with a diameter of 35 cm and a height of 12 cm, and grow and reproduce to form a miniature seed lotus root. The specific steps are:

[0035] Step 1: Place the container and fill the substrate: clean up the weeds in the field, level the field, place the container, and fill the container with soil (mud). The diameter of the container is 30cm, the height is 12cm, and the soil (mud) is filled to 2 / 3 of the depth of the container.

[0036] Step 2: Planting: The daily average temperature is stable above 13°C, and the terminal buds of the mini lotus root of Elian No. 5 are cultivated in the prepared container.

[0037] Step 3: Add water or nutrient solution: Add 2cm deep water to the container to make the plants grow normally.

[0038] Step 4: Fine management: During the growth and development of the miniature lotus root, water depth adjustment, top dressing, weed removal, pest control an...

Embodiment 3

[0042] 1. Stem tip initiation: Take the cleaned terminal buds of the disease-free Elian No. 5 variety, soak them in 75% alcohol for 30 seconds, and then soak them in 0.1% HgCl 2 Soak in the solution for 3 to 12 minutes, then rinse with sterile water for 3 times, each time for 3 to 5 minutes. After completing the above surface disinfection treatment procedure, peel off the outer leaf sheath with a scalpel, cut off a 0.3-0.6cm long shoot tip, and inoculate it in lotus root shoot tip differentiation medium 1 / 2MS+6-BA2.0mg / L+NAA0.5mg / L+3.0% sugar, at a temperature of 24-26°C and a light intensity of 2000-2500 lx, and light for 10 hours a day, buds or clusters of buds will appear after 30-45 days. The pH value of the lotus root shoot tip differentiation medium is 5.8-6.0.

[0043] 2. Subculture: transfer the buds or clumps of buds to the subculture medium 1 / 2MS+6-BA1.0mg / L+NAA0.5mg / L+3.0%sugar for subculture, at a temperature of 24-26°C , under the light intensity of 2000-2500lx...

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Abstract

The invention discloses a miniature lotus root seed breeding technique, and belongs to the technical field of vegetable seedling production. The method comprises the following steps that: tissue culture seedlings of lotus root, test-tube lotus root, terminal buds and axillary buds of lotus root, lotus seeds and the like are cultured in containers with the diameter of 15 to 50cm and the height of 8 to 60cm, and grow and develop into miniature lotus root seeds. The miniature lotus root seed breeding technique comprises the following steps of: preparing soil, arranging containers, and filling soil (mud or other substrates) until the soil is 2 / 3 of volumes of the containers; after the mean daily temperature is stably higher than 13 DEG C, culturing lotus root brood bodies in the prepared containers; adding water or nutrient solution into the containers; performing management such as water depth regulation, top application, weed removal, pest control and the like in the growth and development process of the miniature lotus root seeds; and breeding to obtain the miniature lotus root seeds with at least 3-5 knots, the length of 12 to 80cm and the mass of 20 to 1,500g. The technique can make the lotus root brood bodies miniaturized.

Description

technical field [0001] The invention relates to a method for multiplying miniature lotus roots, and belongs to the technical field of vegetable seedling production. technical background [0002] Lotus root is an important aquatic economic crop, which not only has edible, ornamental and medicinal value, but also has environmental ecological value such as horticultural beautification and water purification. At the same time, lotus root is one of the most distinctive aquatic vegetable crops in my country with the largest cultivation area. The annual cultivation area of ​​lotus root is about 5 million mu, so there is a great demand for lotus root seeds. In recent years, with the adjustment of the agricultural industrial structure, the economic and social value of the lotus root has become more and more prominent. Many rice fields and fish ponds have also been used by farmers to grow lotus roots. However, in the old lotus root production areas, the same variety is planted every ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00A01G1/00
Inventor 柯卫东彭静刘玉平刘义满黄新芳叶元英李双梅李峰朱红莲黄来春赵春李明华
Owner WUHAN VEGETABLE RES INST
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