Method for culturing strong seedling of potato virus-free tube seedling

A technology of potato and test-tube seedlings, which is applied in horticultural methods, botany equipment and methods, cultivation, etc., can solve the problems of unsuitability for large-scale seedling cultivation, affecting the inoculation of virus-free seedlings, and excessive growth of test-tube seedlings, etc., so as to improve the transplanting efficiency. Effects of survival rate, short culture cycle and short internode

Inactive Publication Date: 2014-02-12
四川省植物工程研究院
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Problems solved by technology

The production of virus-free potato seedlings using traditional medium is prone to excessive growth of test-tube seedlings, small stem diameter, long internodes, small leaves, "burnt tips" and many fibrous roots, which not only affect the virus-free seedlings. The working efficiency of inoculation and transplanting reduces the reproduction coefficient and transplanting survival rate of virus-free seedlings. Therefore, it is particularly important to conduct research on promoting the growth of virus-free test-tube seedlings of potato, so as to cultivate robust and high-quality virus-free test-tube seedlings.
[0003] Chinese patent application CN200710066315.6 discloses a method for floating seedlings of potato detoxification tube tubers, which improves the emergence rate of tube tubers, but needs to build seedling ponds, the cost is high, it is not suitable for large-scale seedling cultivation, and the operation is inconvenient
Chinese patent application CN201210450346.2 discloses a cycle production process of virus-free potato test-tube tubers, including the following steps: test-tube plantlets strong seedling cultivation, test-tube tuber induction, after the test-tube plantlets strong seedlings training step, the test-tube plantlets strong seedlings are cultivated After cutting the terminal buds of the virus-free potato plantlets, add the virus-free potato plantlets without the terminal buds to the test-tube tube induction medium to induce the production of test-tube tubers; the clipped terminal buds continue to be cultivated for strong seedlings of the test-tube plantlets. The formula of solid strong seedling medium in this process is MS+10mg.L -1 B9+3.5g / L agar powder+30g / L sugar, the formula in the induction medium is MS+8mg.L -1 BA+100g / L sugar, KNO in MS medium 3 , NH 4 NO 3 , KH 2 PO 4 ,MgSO 4 ,CaCl 2 The concentration is higher, the cost is higher, the process cycle is long, and the liquid strong seedlings are cultivated for about 25 days

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  • Method for culturing strong seedling of potato virus-free tube seedling
  • Method for culturing strong seedling of potato virus-free tube seedling
  • Method for culturing strong seedling of potato virus-free tube seedling

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Experimental program
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Effect test

Embodiment 1

[0018] Example 1 Rapid Propagation and Transplanting of Virus-free Potato Seedlings

[0019] (1) Preparation of medium:

[0020] The preparation contains 950mg.L -1 KNO 3 , 825mg.L -1 NH 4 NO 3 , 100mg.L -1 K H 2 PO 4 ,185mg.L -1 MgSO 4 ,220mg.L -1 CaCl 2 , 27.8mg.L -1 FeSO 4 ·7H 2 O, 37.3mg.L -1 Na 2 ·EDTA·2H 2 O, 0.83mg.L -1 KI, 6.2mg.L -1 h 3 BO 3 , 22.3mg.L -1 MnSO 4 4H 2 O, 8.6mg.L -1 ZnSO 4 ·7H 2 O, 0.25mg.L -1 Na 2 MO 4 2H 2 O, 0.025mg.L -1 CuSO 4 ·5H 2 O, 0.025mg.L -1 CoCl 2 2H 2 O, 100mg.L -1 Inositol, 2mg.L -1 Glycine, 0.1mg.L -1 Thiamine Hydrochloride, 0.5mg.L -1 Pyridoxine hydrochloride, 0.5mg.L -1 Niacin, 30g / L edible sugar, 5.4g / L agar, 0~25mg.L -1 For B9 medium, adjust the pH to 5.8;

[0021] (2) Rapid propagation of virus-free seedlings:

[0022]Insert each stem node as an explant forward into the culture medium in step (1), and cultivate it for 15 days at 23°C under 2000 lx light conditions, and count the stem length...

Embodiment 2

[0030] Example 2 Rapid Propagation and Transplanting of Virus-free Potato "Mira" Seedlings

[0031] (1) Preparation of medium:

[0032] The preparation contains 950mg.L -1 KNO 3 , 825mg.L -1 NH 4 NO 3 , 100mg.L -1 K H 2 PO 4 ,185mg.L -1 MgSO 4 ,220mg.L -1 CaCl 2 , 27.8mg.L -1 FeSO 4 ·7H 2 O, 37.3mg.L -1 Na 2 ·EDTA·2H 2 O, 0.83mg.L -1 KI, 6.2mg.L -1 h 3 BO 3 , 22.3mg.L -1 MnSO 4 4H 2 O, 8.6mg.L -1 ZnSO 4 ·7H 2 O, 0.25mg.L -1 Na 2 MO 4 2H 2 O, 0.025mg.L -1 CuSO 4 ·5H 2 O, 0.025mg.L -1 CoCl 2 2H 2 O, 100mg.L -1 Inositol, 2mg.L -1 Glycine, 0.1mg.L -1 Thiamine Hydrochloride, 0.5mg.L -1 Pyridoxine hydrochloride, 0.5mg.L -1 Niacin, 30g / L edible sugar, 5.4g / L agar, 0~25mg.L -1 For B9 medium, adjust the pH to 5.8;

[0033] (2) Rapid propagation of virus-free seedlings:

[0034] Insert each stem node as an explant forward into the culture medium in step (1), and cultivate it for 15 days at 23°C under 2000 lx light conditions, and count the ste...

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Abstract

The invention relates to the technology of strengthening seedlings of potato virus-free tube seedlings, and particularly relates to a method for culturing a strong seedling of a potato virus-free tube seedling. The method mainly comprises the following steps: preparation of a culture medium; rapid propagation of a virus-free seedling; transplanting of the virus-free seedling. According to the method, B9 with different concentrations are added in different stages of tissue culture and rapid propagation of potato by taking an improved 1/2 MS as a culture medium and tap water and edible white sugar as raw materials, so that the subculture period is shortened and the test-tube seedling is more robust. The concentration of the B9 added in the rapid propagation stage of the virus-free tube seedling is 4-8mg/L, so that stem diameter, number of effective stems and number of roots of the virus-free tube seedling are effectively promoted. The blade is thickened and deeply green. The subculture period is shortened from conventional 3-4 weeks to just 14-18 days, so that the propagation efficiency of the potato virus-free tube seedling is extremely improved. B9 with concentration of 10-15mg/L is added into the culture medium before the virus-free seedling is transplanted, so that the steam of the virus-free tube seedling is thicker and stronger, shorter in internode and higher in degree of lignification of the stem, and the transplanting survival rate of the seedling can be improved.

Description

technical field [0001] The invention relates to a technique for growing strong seedlings of potato virus-free test tube seedlings, in particular to a method for cultivating strong seedlings of potato virus-free test-tube seedlings. Background technique [0002] The establishment of potato virus-free and seed potato production system has fundamentally solved the problem of potato seed degradation, and the production and rapid propagation of virus-free test-tube seedlings is one of the most critical links in the whole system. The production of virus-free potato seedlings using traditional medium is prone to excessive growth of test-tube seedlings, small stem diameter, long internodes, small leaves, "burnt tips" and many fibrous roots, which not only affect the virus-free seedlings. The working efficiency of inoculation and transplanting reduces the reproduction coefficient and transplanting survival rate of virus-free seedlings. Therefore, it is particularly important to carry...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 秦廷豪阳翠黄运军李晓梅龙小蓉彭晓玲
Owner 四川省植物工程研究院
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