The invention provides a method for breeding tridentiger trigonocephalus by artificial induced spawning and
insemination, which relates to the artificial propagation of gobies. The method required tobe provided for artificially breeding the tridentiger trigonocephalus comprises the steps of ripe fish screening, maturity acceleration,
insemination, incubation and larval fish culture, wherein the maturity acceleration of ripe fishes uses luteotropin to release
hormone analogues,
domperidone maleate and chorionic
gonadotropin, and is characterized in that the three hormones are used twice for the maturity acceleration; in the maturity acceleration, the
water temperature of culture is between 18 and 21 DEG C, the
salinity is between 2 and 6, the dissolved
oxygen is between 3.5 and 6.5mg / L, and the pH is between 7.5 and 9.0; and after the second time of the maturity acceleration, in 8 to 10 hours, mature ova and mature sperms are selected for
artificial insemination which comprises the following steps that: the activated sperms are poured on the ova for sufficient contact so as to obtain fertilized ova; the fertilized ova are placed in a
hatchery pond for incubation; and the hatched larval fish is placed in a culture pond for incubation, and
chlorella and
yolk powder of eggs are placed in water from the third day after the larval fish is hatched to feed food for the larval fish. The method is used for the artificial breeding of the tridentiger trigonocephalus.