Electrochemical immunosensor for detecting H5N1 subtype avian influenza viruses and manufacturing method of electrochemical immunosensor

An avian influenza virus, electrochemical technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., can solve the problems of destroying the graphite SP2 hybrid system, and the conductivity of graphene oxide is much poorer, achieving poor conductivity, high sensitivity, good water solubility
CN102998447AActive Publication Date: 2013-03-27GUANGXI VETERINARY RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUANGXI VETERINARY RES INST
Publication Date
2013-03-27

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Abstract

The invention discloses an electrochemical immunosensor for detecting H5N1 subtype avian influenza viruses and a kit. An antibody A-graphene oxide-BSA (Bovine Serum Albumin) compound for detecting the H5N1 avian influenza viruses provided by the invention is formed by an antibody A of the H5N1 avian influenza viruses and bovine serum albumin which are connected with graphene oxide through amido bonds. Proofed by an experiment provided by the invention, the nanometer antibody A-graphene oxide-BSA compound has an amplifying function, and the detection kit of the super-sensitive electrochemical immunosensor is established on the basis of a signal amplification property of the nanometer compound; and when the content of the H5N1subtype avian influenza viruses in a sample is small, a signal is amplified by the nanometer antibody A-graphene oxide-BSA compound, thereby fulfilling the purpose of effective analysis and detection. The method provided by the invention has the advantages of simple operation, high sensitivity and high specificity. According to the characteristics of the electrochemical immunosensor, if other antibodies are selected in experiments, the method can be used for detecting other pathogenic microorganisms.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to an electrochemical immunosensor for detecting H5N1 subtype avian influenza virus and a preparation method thereof. Background technique

[0002] Avian influenza (Avian influenza, AI) not only seriously affects the development of poultry industry, but also endangers human health. According to the World Health Organization (WHO), from November 2003 to November 2012, there were 606 cases of H5N1 infection in humans, 357 cases died, and the mortality rate was as high as 58.9%. Therefore, it is of great significance to establish a rapid, sensitive and specific method for detecting H5N1 to prevent and control the spread of H5N1 virus.

[0003] At present, the methods used to detect AIV include virus isolation and identification, enzyme-linked immunosorbent assay (ELISA), hemagglutination (HA) and hemagglutination inhibition (HI), reverse transcription polymerase chain reaction (RT-PCR), n...

Claims

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