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Application of thelephora ganbajun in preparation of antitumor drugs

An anti-tumor drug, the technology of scalloped bacteria, which is applied in the chemical industry, can solve the problems that have not been reported on the anti-tumor effect of scallopedicin, and achieve the effect of inhibiting proliferation and tumor inhibition.

Inactive Publication Date: 2012-06-13
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, so far, there has been no report on the anti-tumor effect of ganbacin

Method used

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  • Application of thelephora ganbajun in preparation of antitumor drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 MTS method to measure the growth inhibitory effect of scadamectin on liver cancer cells

[0032] HGC cells (purchased from the Cell Bank of the Chinese Academy of Sciences) 3 × 10 3 Each well was inoculated into a 96-well plate, cultured for 24 hours to allow it to adhere to the wall, and then added ganbacin (purchased from Shanghai Institute of Materia Medica, Chinese Academy of Sciences), with 6 concentration gradients and 3 replicate wells for each concentration. Cells at 37°C, 5% CO 2 After culturing for 72 hours under the same conditions, the culture medium was discarded, and the cell viability was measured with an MTS kit (Promega).

[0033] The test method is: wash the cells once with serum-free medium, and add the pre-prepared MTS chromogenic solution according to the amount of 100 μl / well (add 2ml of solution 1 and 100μl of solution 2 to 10ml of serum-free medium, and mix well). A well without cells was set as the background well to correct for the ...

Embodiment 2

[0036] Example 2 The growth inhibitory effect of scadamectin on human pancreatic cancer cells

[0037] Using cck-8 kit (Japan Dojin Chemical Research Institute) detection.

[0038] step:

[0039] 1) PANC-1 cells (purchased from ATCC) were evenly planted in a 96-well plate, with 3000 cells per well.

[0040] 2) Wait for the wall to adhere, and add medicine after overnight.

[0041] 3) After culturing for 48 hours, replace the complete medium with a mixture of serum-free medium and CCK8 (10:1), and incubate in a 37°C incubator for 2 hours.

[0042] 4) Take 450nm as the measurement wavelength and 650nm as the reference wavelength, and measure the reading on a microplate reader.

[0043] Results: The IC50 value of ganbacin on PANC-1 cells was 3.29 μM.

Embodiment 3

[0044] Example 3 The growth inhibitory effect of ganbamectin on human lung adenocarcinoma cells

[0045] With the method of Example 2, the measured IC50 value of scadamectin on A549 cells was 8.54 μM.

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Abstract

The invention belongs to the chemical field and the medical field, and relates to application of thelephora ganbajun in preparation of antitumor drugs. Tumor cells comprise hepatocellular carcinoma cells, lung adenocarcinma cells and pancreatic cancer cells. The thelephora ganbajun belongs to a natural product, is small in toxic and side effects, high in bioavailability and stable in performance,and has clinical service values. Small molecular compounds are developed as novel antitumor drugs or auxiliary elements of the novel antitumor drugs, are obvious in antitumor effect and are environment-friendly. The application of thelephora ganbajun in preparation of antitumor drugs provides a novel way and a novel means for treating and curing tumor.

Description

technical field [0001] The invention belongs to the fields of chemical industry and medicine, and relates to the application of stilamectin in the preparation of antitumor drugs. Background technique [0002] Ganba fungus is a rare wild edible fungus unique to Yunnan Province, which cannot grow in other provinces in China or abroad. Scientific name: hydrangea, also known as the flower fungus, horse tooth fungus and so on. It grows among the pine trees in the mountain forests in central and western Yunnan, and is the top grade of wild edible fungi. This fungus has no cap and gills, and clusters like bovine teeth, so it is commonly known as bovine tooth fungus. When first unearthed, this fungus is yellow-brown, and when it is mature, it turns dark brown and has a strong aroma similar to corned beef jerky. Because corned beef jerky is also called dry bark, the fungus is named dry bark fungus. [0003] Ganbamectin has strong antibacterial activity, and it is also an important...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P35/00
Inventor 余龙刘祖龙胡立宏
Owner FUDAN UNIV
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