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Thelephora ganbajun mycelium polysaccharide as well as preparation method and application of thelephora ganbajun mycelium polysaccharide

A technology of mycelium polysaccharide and dry bacteria, which can be used in biochemical equipment and methods, microorganism-based methods, pharmaceutical formulations, etc., can solve the problems of insufficient utilization and the like

Active Publication Date: 2017-05-31
济南健康产业科技研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem of insufficient utilization of the dry bacteria polysaccharide in the above prior art in extraction and application, the application provides a bacteria strain composed of dry bacteria ( Thelephoraganbajun Zang) Mycelia polysaccharide obtained by TG-1 fermentation

Method used

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  • Thelephora ganbajun mycelium polysaccharide as well as preparation method and application of thelephora ganbajun mycelium polysaccharide
  • Thelephora ganbajun mycelium polysaccharide as well as preparation method and application of thelephora ganbajun mycelium polysaccharide
  • Thelephora ganbajun mycelium polysaccharide as well as preparation method and application of thelephora ganbajun mycelium polysaccharide

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The acquisition of embodiment 1 bacterial strain

[0043]Collect 4 fresh fruiting bodies of D. spp. from different areas in Yunnan Province, disinfect the surface with 75% alcohol, rinse several times with sterile water, use sterilized tweezers to tear off small pieces of the internal tissue of D. spp. and inoculate them with chloramphenicol On the potato dextrose (PDA) medium plate, move it into a 24 ℃ incubator for cultivation. After the mycelium grows, it will be purified repeatedly until the pure culture of D. spp. is obtained. Four strains of S. spp. were screened from 4 S. spp. fruiting bodies and named as TG-1, TG-2, TG-3 and TG-4 respectively.

Embodiment 2

[0044] The liquid fermentation of embodiment 2 dried bacteria

[0045] Carry out fermenter culture experiments, ① Activation of strains. The bacteria strains TG-1, TG-2, TG-3 and TG-4 were respectively inoculated on the activation medium plate (potato 200 g / L, glucose 20 g / L, magnesium sulfate 1 g / L, potassium dihydrogen phosphate 1.5 g / L), cultured at 24°C for 7 days. Take 0.5 cm 2 The activated strain of D. spp. was inserted into the liquid medium (potato 200 g / L, glucose 20 g / L, peptone 2 g / L, magnesium sulfate 1 g / L, potassium dihydrogen phosphate 1.5 g / L, pH natural), The volume of the 500 mL Erlenmeyer flask was 300 mL, and then cultured with shaking on a shaker at 24 °C for 10 days. ② Fermentation tank culture conditions. Loading capacity: 7.5 liters; Inoculation amount: 10%; Fermentation temperature: 24 ℃; Stirring speed: 350 r / min; Foam control: Add two drops of defoamer after inoculation. ③ Determination of pH value and DO value during fermentation. The pH elec...

Embodiment 3

[0050] The extraction of embodiment 3 dried bacteria mycelium polysaccharides

[0051] 1) Separation of the mycelia of D. spp.:

[0052] Centrifuge the dry bacteria culture (10000 r / min, 10 min), collect the mycelium, and wash the mycelium 3 times. The mycelium was dried and pulverized at 55°C to obtain the mycelium powder of D.

[0053] 2) Extraction of polysaccharides from the mycelium of Drosophila:

[0054] Take the mycelium powder of D. spp., mix the mycelium powder with deionized water at a ratio of 1:20, adjust the pH value to 8, and ultrasonically pulverize it with an ultrasonic power of 600 W for 10 min, and then place it at 90 °C In a water bath, extract for 2 h, centrifuge to get the supernatant. The mycelium residue after centrifugation was extracted twice according to the above steps, and the supernatant was combined.

[0055] 3) Purification of polysaccharides from the mycelia of Drosophila:

[0056] Concentrate the polysaccharide extract to 1 / 2 of its origi...

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Abstract

The invention relates to the technical field of thelephora ganbajun polysaccharide, and particularly relates to a thelephora ganbajun mycelium polysaccharide. The preservation number is CGMCC No.12977; a thelephora ganbajun strain TG-1 is fermented in a liquid culture medium; and polysaccharide is extracted from mycelium and is eluted by using deionized water and 0.05, 0.1 or 0.3mol / L of NaCl solution after chromatographic separation to obtain MPS-1, MPS-2, MPS-3 and MPS-4 separately. The mycelium grows vigorously when the TG-1 is fermented and cultivated in liquid, and the mycelium is high in polysaccharide yield, and is of very great significance in development and utilization of thelephora ganbajun and massive production of the thelephora ganbajun mycelium polysaccharide. The antioxidant ability of the MPS-2 is the highest, the antioxidant ability of the MPS-3, the MPS-4 and the MPS-1 are secondary, and a theoretical basis is laid for activity analysis of the polysaccharide and development of polysaccharide foods with an anti-aging function; and the thelephora ganbajun mycelium polysaccharide also has excellent moisturizing ability.

Description

technical field [0001] The present invention relates to the technical field of the dry mushroom polysaccharide, in particular to a dried dry mushroom mycelium polysaccharide, and also relates to a preparation method and application thereof. Background technique [0002] Dried bacteria ( Thelephora ganbajun Zang) is a rare wild edible fungus of the genus Thelephora unique to my country. It has a good taste and high nutritional value. It is only distributed on the weakly acidic red soil ground in the high altitude area of ​​800-2300 m in central Yunnan, my country. There is an ectomycorrhizal relationship between D. spp. and pine trees, and it is difficult to succeed in artificial cultivation. As a result, the phenomenon of disorderly harvesting of D. spp. is serious, and the output decreases year by year. Every year, D. spp. is always in short supply when it comes to the market, and the price increases year by year. Due to the limitation of its distribution, the researc...

Claims

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Application Information

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IPC IPC(8): C12P19/04A23L31/00A23L33/125A61K8/9728A61K8/73A61Q19/00A61Q19/08C12R1/645
Inventor 马耀宏郑岚杨俊慧蔡雷高广恒史建国
Owner 济南健康产业科技研究院
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