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Method of quickly acquiring Tilia plant primary aseptic material

A technology of the Tilia genus and plants, applied in the field of quickly obtaining the first-generation aseptic materials of the Tilia genus plants, to achieve the effects of saving production costs, high germination rate, and avoiding pollution problems

Inactive Publication Date: 2019-03-19
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] For above-mentioned deficiencies in the prior art, the present invention provides a kind of method that obtains the first-generation aseptic material of Tilia plant quickly, the present invention uses river sand as the first-generation aseptic seedling culture substrate, which is conducive to quickly removing the dormancy of Tilia seeds, It can also greatly save production costs, and at the same time effectively avoid the pollution problem in tissue culture, with a high seedling yield and good practical application prospects

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  • Method of quickly acquiring Tilia plant primary aseptic material

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Embodiment 1

[0040] A method for rapidly obtaining the first generation of Tilia aseptic material, the specific steps comprising:

[0041] (1) Culture substrate treatment: mix river sand with water and keep the humidity at 65%; divide the wet sand into culture bottles for autoclaving, the sterilization method is: 121°C, 0.12Mpa / cm 2 Sterilize for 20 minutes;

[0042] (2) Linden tree seed treatment: a. put the seeds into a sterile bottle, pour in concentrated sulfuric acid (the amount of concentrated sulfuric acid should not pass through the seeds) and process for 15 minutes, during which the glass rods are constantly used to stir; b. drain Concentrated sulfuric acid, rinse the seeds with sterile water several times until the residue of concentrated sulfuric acid is rinsed; c. Soak the seeds in 0.1% mercuric chloride solution for 5 minutes, and rinse them with sterile water for 5 times; d. Use sterile 500mg / L GA 3 Soak the seeds in the solution for 8 hours (the seed treatment process is a...

Embodiment 2

[0046] A method for rapidly obtaining the first generation of Tilia aseptic material, the specific steps comprising:

[0047] (1) Culture substrate treatment: mix river sand with water and keep the humidity at 60%; divide the wet sand into culture bottles for autoclaving, the sterilization method is: 121°C, 0.12Mpa / cm 2 Sterilize for 20 minutes;

[0048] (2) Linden tree seed treatment: a. put the seeds into a sterile bottle, pour in concentrated sulfuric acid (the amount of concentrated sulfuric acid should not pass through the seeds) and process for 15 minutes, during which the glass rods are constantly used to stir; b. drain Concentrated sulfuric acid, rinse the seeds with sterile water several times until the residue of concentrated sulfuric acid is rinsed; c. soak the seeds in 0.1% mercuric chloride solution for 5 minutes, and rinse with sterile water for 5 times; d. use sterile 200mg / L GA 3 Soak the seeds in the solution for 10 hours (the seed treatment process is all car...

Embodiment 3

[0052] A method for rapidly obtaining the first-generation aseptic material of Tilia bran, the specific steps comprising:

[0053] (1) Culture substrate treatment: mix river sand with water and keep the humidity at 60%; divide the wet sand into culture bottles for autoclaving, the sterilization method is: 121°C, 0.12Mpa / cm 2 Sterilize for 20 minutes;

[0054] (2) Linden tree seed treatment: a. put the seeds into a sterile bottle, pour in concentrated sulfuric acid (the amount of concentrated sulfuric acid should not pass through the seeds) and process for 15 minutes, during which the glass rods are constantly used to stir; b. drain Concentrated sulfuric acid, rinse the seeds with sterile water several times until the residue of concentrated sulfuric acid is rinsed; c. soak the seeds in 0.1% mercuric chloride solution for 5 minutes, and rinse them with sterile water for 5 times; d. use sterile 800mg / L GA 3 Soak the seeds in the solution for 7 hours (the seed treatment process...

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Abstract

The invention provides a method of quickly acquiring a Tilia plant primary aseptic material. The method includes: S1, treating a culture medium, to be specific, disinfecting wet sand to obtain a linden seed culture medium; S2, treating linden seeds, to be specific, using concentrated sulfuric acid, mercury dichloride and gibberellin successively to treat linden seeds; S3, acquiring an aseptic material, to be specific, culturing the linden seeds treated in step S2, in the culture medium of S1. River sand is utilized herein as a culture medium for primary aseptic seedlings; dormancy of linden seeds can be quickly eliminated, production cost can be greatly reduced, the pollution problem in tissue culture can be effectively avoided, seedling yield is high, and the method has a good actual application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant propagation, and in particular relates to a method for rapidly obtaining primary aseptic materials of Tilia plants. Background technique [0002] The genus Tilia (Tilia L.) belongs to the deciduous trees of Tiliaceae. It has a beautiful tree shape, most of which are majestic, with large leaves and thick shade, few pests and diseases, and strong resistance to smoke and harmful gases. It is an excellent landscaping tree species and is known as the world's One of the four broad-leaved street trees, many species are still important timber species. Its wood is white, soft, elastic, not warped or cracked, and can be used for a variety of materials. Its flowers are fragrant and fragrant, and it is the main nectar source plant. In addition, Linden Tree flowers and leaves contain flavonoids, coumarins, lactones and other secondary substances, which have high medicinal value. However, due to its great medici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 王因花孔雨光燕丽萍鲁仪增刘翠兰李庆华任飞梁静梁燕吴德军
Owner SHANDONG FOREST SCI RES INST
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