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Method for extracting alga auxin from alga

A technology for extracting seaweed and auxin, applied in chemical instruments and methods, preparation of organic compounds, preparation of amino compounds, etc., can solve the problems of late research work, low auxin, complex process, etc., to achieve low cost and promote crop growth. , the effect of wide practicability

Inactive Publication Date: 2006-01-04
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Because the content of auxin in seaweed is very low, its extraction amount is low, and the process is complicated, (Crouch, I.J., J.V.Staden, 1993, Evidence for the presence of plant regulators incommercial seaweed products, Plant Growth Regulation, 13: 21-29 .Jacobs, W.P., K.Falkenstein and R.H.Hamilton, 1985, Nature and amount of auxin inalgae, Plant Physiol, 78:844-848.) At present, my country's research work on extracting auxin from seaweed is relatively late until 1995 This part of the work was gradually carried out after the Institute of Oceanology of the Chinese Academy of Sciences undertook the research projects of the National "Ninth Five-Year Plan"

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Take 100 kg of artificially cultivated centipede algae (or kelp (brown algae), or red algae such as Polytubella), wash it with sea water (do not use fresh water), remove impurities and foreign objects, put it into a colander (net), and turn it over To ensure that the surface of fresh seaweed is dry, add 30g of seaweed polyphenol antioxidant (or add 0.2g / Kg of seaweed polyphenol antioxidant and 0.1g / Kg tert-butyl p-hydroxyanisole) and 160 liters of 75% methyl alcohol After the solution is stirred evenly, it is sealed, stirred and extracted in a dark room for 8 hours, filtered, and the filter residue is washed with 10 liters of pure methanol. 1.1Kg.

Embodiment 2

[0019] Take 100 kg of artificially cultivated kelp (or red algae such as polycystic algae and centipede algae), wash it with sea water (do not use fresh water), remove impurities and foreign objects, put it into a colander (net), and turn it over to ensure fresh seaweed The surface is dry, and then it is put into a 2cm-scale shredder for chopping, and the block seaweed after chopping is put into a stainless steel container with a stirrer and a flow port device with a diameter of 120cm, and 30g of seaweed polyphenol antioxidant is added (or add 0.3g / Kg tert-butyl-p-hydroxyanisole) after stirring evenly, add 80 liters of 78% methanol solution, stir for 30min and mix evenly. The mixed solution is transferred from the flow port to a homogenizer, and crushed for 3 minutes (homogenization can be 3-5 minutes). Add 80 liters of 78% (can be volume concentration 75-80%) methanol solution again in the homogeneous solution, move to the dark room and continue to stir and extract for 3 hour...

Embodiment 3

[0021] Take 100 kg of artificially cultured Polytubule algae (or kelp (brown algae), or red algae such as centipede algae), wash it with sea water (do not use fresh water) to remove impurities and foreign objects, put it into a colander (net), and turn it over to Make sure that the surface of fresh seaweed is dry, then directly put it into a stainless steel container with a diameter of 150cm and a stirrer and a flow port device, add 40g of seaweed polyphenol antioxidant, stir evenly, then add 100 liters of 80% methanol solution, stir After 1 hour, move it to a homogenizer, crush (homogenize) for 8 minutes; add 80 liters of 80% methanol solution to the homogeneous solution again, move to a dark room and continue stirring and extracting for 6 hours, filter, and filter the residue with pure methanol 10 liters were washed, and the filtrate was rotary evaporated to about 10 liters at 45° C., and then freeze-dried to obtain 1 kg of dry seaweed auxin.

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Abstract

The present invention relates to natural algae auxin, and is especially method of extracting algae auxin from algae. Algae is first washed with sea water to eliminate surface small life and impurity and has its surface water eliminated, algae poly phenol antioxidant in the amount of 0.2-0.4g / kg algae and methanol in the amount of 1.4-1.8g / kg algae are then added and mixed with algae to extract under stirring inside dark room, and through filtering and evaporating concentration to obtain the concentrated matter as the algae auxin. The present invention has the advantages of simple and practical process, low cost, short preparation period, etc.

Description

technical field [0001] The invention relates to natural seaweed auxin (indole acetic acid, IAA), in particular to a method for extracting seaweed auxin (indole acetic acid) from seaweed. Background technique [0002] Auxin is an important class of endogenous plant hormones, which widely exist in aquatic and terrestrial plants, and play a vital role in their growth and development. Existing studies have shown that auxin can stimulate crop root development and cold resistance, and it can greatly improve the survival rate when cutting plants. Auxin was first isolated and crystallized from human urine and yeast in 1934. At that time, it was named heteroauxin (heteroauxin). Now this name has been abandoned in European and American materials, and it is collectively called auxin. The most common auxin is indole-3-acetic acid (IAA). Many seaweeds themselves contain auxin and auxin-like compounds. Van Overbeek announced as early as 1940 that auxin is widely present in many seaweed...

Claims

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Application Information

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IPC IPC(8): C07C209/12
Inventor 韩丽君范晓房国明
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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