Seedling hardening and transplant acclimatization method for roxburgh anoectochilus terminal bud tissue culture seedlings

The technology of tissue culture and hardening is applied in the field of plant seedling breeding, and can solve the problems of low survival rate of tissue culture seedlings transplanted, poor self-supporting ability of tissue culture seedlings, and low survival rate of transplanting, etc. Achieve the effect of improving the transplanting survival rate, easy to grasp, and low disease incidence.

Inactive Publication Date: 2015-06-10
ZHEJIANG FORESTRY UNIVERSITY
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  • Description
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AI Technical Summary

Problems solved by technology

At present, the field production of clematis is mainly carried out by cultivating tissue culture seedlings for propagation. The tissue culture seedlings just produced from the tissue culture room have poor self-support ability and poor adaptability to the outside world, resulting in a low survival rate of transplanting ( Usually only 50-60%)
Seedling hardening is an important step before going from the tissue culture room to field cultivation. However, according to the current hardening method, the survival rate of transplanted clematis tissue culture seedlings is not high, which keeps the production cost at a relatively high level.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Implementation location: Baicao Garden of Zhejiang A&F University

[0020] (2) Implementation time: March 2013-April 2013

[0021] (3) Implementation plan: ①Selection of seedling training ground: choose a flat land with good ecological conditions, clean water source, good drainage, open site, and ventilation. There are no industrial factories, mines, "three wastes" pollution, garbage dumps and other sources of pollution within 5km around , and away from the area beyond 500m from the main traffic road; 2. Construction of the seedling hardening greenhouse: the length of the seedling hardening greenhouse is 30m, the width is 8m, and the height is 3.3m. Plastic film and 80% shading rate are installed on the greenhouse Sunshade nets, seedbeds are set in parallel in the greenhouse, the width of the seedbeds is 2m, and the height is 0.8m; 3. the selection of tissue culture seedlings in bottle seedlings: Clematis clematis tissue culture seedlings require good growth, leaf s...

Embodiment 2

[0024] (1) Implementation location: Baicao Garden of Zhejiang A&F University

[0025] (2) Implementation time: March 2014-April 2014

[0026] (3) Implementation plan: ①Selection of seedling training ground: choose a flat land with good ecological conditions, clean water source, good drainage, open site, and ventilation. There are no industrial factories, mines, "three wastes" pollution, garbage dumps and other sources of pollution within 5km around , and away from the area beyond 500m from the main traffic road; 2. Construction of the seedling hardening greenhouse: the length of the seedling hardening greenhouse is 30m, the width is 8m, and the height is 3.3m. Plastic film and 80% shading rate are installed on the greenhouse Sunshade nets, seedbeds are set in parallel in the greenhouse, the width of the seedbeds is 2m, and the height is 0.8m; 3. the selection of tissue culture seedlings in bottle seedlings: Clematis clematis tissue culture seedlings require good growth, leaf s...

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Abstract

The invention relates to a seedling hardening and transplant acclimatization method for roxburgh anoectochilus terminal bud tissue culture seedlings and aims to obviously increase the transplant survival rate of the roxburgh anoectochilus terminal bud tissue culture seedlings. The method has the characteristics of high economical efficiency, practicability, high simpleness in operation, high easiness in mastering, high standardability, high efficiency and the like. According to the technical scheme, the method comprises the following steps: firstly, selecting a seedling hardening field; secondly, constructing a seedling hardening greenhouse; thirdly, selecting tissue culture bottle seedlings; fourthly, selecting seedling hardening matrix; fifthly, management before the bottle seedlings are taken out of bottles; sixthly, washing the seedlings out of the bottles; seventhly, transplanting and management.

Description

technical field [0001] The invention relates to a method for hardening and transplanting domestication of tissue cultured plantlets of plants, in particular to a method for hardening and transplanting domestication of tissue cultured plantlets of A. auranthus clematis, belonging to the technical field of plant seedling breeding. Background technique [0002] Anoectochilus roxburghii (Anoectochilus roxburghii), also known as golden thread orchid and golden silk grass, is a perennial precious Chinese herbal medicine of the family Orchidaceae. It is mainly produced in Fujian, Zhejiang, Taiwan, Jiangxi, Guizhou and other places. It has detoxification and other effects, and is used to treat diabetes, nephritis, acute and chronic hepatitis and other diseases, and enjoys the laudatory title of "King of Medicine" among the people. Modern research shows that clematis contains polysaccharides, flavonoids, steroids, terpenes, alkaloids and other active ingredients, which have the pharm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01H4/00
CPCA01G22/00A01G24/00A01G31/00
Inventor 邵清松叶申怡易浩君张爱莲
Owner ZHEJIANG FORESTRY UNIVERSITY
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