Method for culturing test tube plantlet of Xingren Anoectochilus
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A cultivation method and test-tube seedling technology, applied in the field of plant tissue cultivation, can solve the problems of endangerment and low seed germination rate, and achieve the effect of being suitable for popularization and application, and the method is simple
Inactive Publication Date: 2011-08-10
SUBTROPICAL CROPS INST OF GUIZHOU PROVINCE
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[0004] The technical problem to be solved by the present invention is to overcome the low germination rate of existing wild A. roxburghii seeds and the defects of being on the verge of extinction, and to provide a method for cultivating test-tube seedlings of A. chrysanthemum Xingren. Th
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[0013] The technical scheme of the present invention is described in detail below in conjunction with specific embodiment, the cultivation method of Xingren Aurea test-tube seedling:
[0014] (1) Selection of explants and establishment of a sterile system: Select robust plants, take 1cm-long stems with axillary buds as explants, clean the stems, and rinse them in washing powder water for 3 to 5 minutes. Minutes, then rinse with running water for 30 minutes, in the ultra-clean workbench, wet with 75% alcohol for 5-8 seconds, sterilize in 0.1% HgCl solution for 9-13 minutes, take it out, rinse with sterile water for 5-6 Second-rate;
[0015] (2) Sprout induction and proliferation culture: inoculate the aseptically treated explants on the bud induction medium for bud induction culture, and generally germinate after 7 to 10 days of inoculation. When it germinates to a length of 0.5 cm, it is cut open, transferred to the proliferation medium for further proliferation culture, and ...
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Abstract
The invention discloses a method for culturing a test tube plantlet of Xingren Anoectochilus, which comprises the steps of: selecting a stem section with an axillary bud as an explant, carrying out collateral bud induction and enrichment culture, grafting the collateral bud into a strong seedling rooting culture medium for strong seedling rooting cultivation, and obtaining the test tube plantlet, wherein the culture medium used for collateral bud induction and enrichment culture is MS+(2-5)mg/l 6-BA (butyl acrylate)+(0.2-0.5)mg/l NAA (naphthyl acetic acid)+8g/l agar+30g/l sugar pH: 5.7-6.0; and the strong seedling rooting medium is (1/2MS-MS)+(0.01-0.05)mg/l 6-BA+(0.05-0.10)mg/l NAA+8g/l agar+(15-25)g/l sugar pH: 5.7-6.0. The Xingren Anoectochilus has the propagation coefficient of more than 10 times, so that the test tube plantlet is thick and strong and has the transplant survival rate of more than 95%.
Description
technical field [0001] The invention relates to the technical field of plant tissue cultivation, in particular to a method for cultivating test-tube seedlings of Xingren clematis. Background technique [0002] Xingren golden clematis ( A. xingrenensis Z.H.Tsi et X.H.Jin) is Orchidaceae (Orchidaceae) Anoectochilus ) perennial herbaceous plant, collectively referred to as clematis chinensis, Taiwan clematis, Kaohsiung clematis, etc., are used as medicine in the folk, which can reduce inflammation, detoxify, stop bleeding and lower blood pressure, strengthen the heart and diuresis, and treat acute hepatitis, Chronic hepatitis, acute and chronic nephritis, renal failure, high blood pressure, diabetes, gynecological diseases, cancer, etc. have very obvious curative effects, so it is known as "the king of medicine, magic medicine, and golden grass" among the people. According to reports, the amino acid composition, composition, content and anti-aging active trace element conte...
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