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Method for preparing hydrogen from straws

A technology of straw and hydrogen, which is applied in the field of using straw for biomass fermentation to prepare hydrogen, can solve problems such as inability to digest straw well, difficulty in hydrogen production, etc., and achieves a technology that is beneficial to the degradation of hydrogen-producing microorganisms, has good effect and high hydrogen yield. Effect

Inactive Publication Date: 2014-02-26
四川蓉加川大环保工程咨询设计有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main reason is that most biological methods cannot digest straw well, because straw is mainly composed of cellulose, hemicellulose and lignin.
Hemicellulose and lignin are covalently bonded in straw to form a complex to embed cellulose in it, making it difficult for cellulase to contact cellulose, so it is difficult to degrade straw to produce hydrogen

Method used

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  • Method for preparing hydrogen from straws
  • Method for preparing hydrogen from straws
  • Method for preparing hydrogen from straws

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. A method for preparing hydrogen from stalks, comprising the following steps:

[0029] 1. Utilize the microbial composite bacterial agent for degrading straw to carry out aerobic degradation of straw: use the patent number CN103194392A invention titled microbial composite bacterial agent for degrading straw and its method for degrading straw; the specific operations are: ① straw pretreatment: the straw Mix with dilute sulfuric acid at a mass ratio of 1:2, heat in an autoclave at a constant temperature of 150°C for 2 hours, then wash the straw with distilled water until the pH of the washing solution is 7, filter, and dry the straw at 60°C for later use; ②Preparation of straw Aerobic degradation medium: first prepare liquid aerobic degradation medium, then add the above-mentioned pretreated straw, add 0.5-1.5 g of straw per milliliter of liquid aerobic degradation medium; each liter of liquid aerobic degradation medium contains Components of the following mass: ammoniu...

Embodiment 2

[0039] 1. Using straw to prepare hydrogen includes the following steps:

[0040] 1. Carrying out aerobic degradation of straw by using a microbial compound agent for degrading straw: the same as in Example 1.

[0041] 2, cow dung pretreatment: with embodiment 1.

[0042] 3. Preparation of straw anaerobic degradation medium: Weigh 5 g of pretreated straw powder into 50 ml of liquid anaerobic degradation medium, and sterilize at 120° C. for 20 minutes. Wherein the components in the liquid anaerobic degradation medium are the same as those in Example 1.

[0043] 4. Add the pretreated cow dung to the straw anaerobic degradation medium in an amount of 1g per gram of straw, pass nitrogen gas for 3 minutes to remove oxygen, seal, avoid light, and shake at 65°C and 120r / min Fermentation and cultivation for 7 days. Simultaneously add the samples without pretreatment straw and no straw as comparative examples.

[0044] 2. The result of straw degradation to produce hydrogen

[0045]...

Embodiment 3

[0050] 1. Using straw to prepare hydrogen includes the following steps:

[0051] 1. Carrying out aerobic degradation of straw by using a microbial compound agent for degrading straw: the same as in Example 1.

[0052] 2, cow dung pretreatment: with embodiment 1.

[0053] 3. Preparation of straw anaerobic degradation medium: Weigh 5 g of pretreated straw powder into 50 ml of liquid anaerobic degradation medium, and sterilize at 120° C. for 20 minutes. Wherein the components in the liquid anaerobic degradation medium are the same as those in Example 1.

[0054] 4. Add the pretreated cow dung to the straw anaerobic degradation medium at an amount of 1.5g per gram of straw, pass nitrogen gas for 3 minutes to remove oxygen, seal, avoid light, shake at 65°C and 120r / min Fermentation culture was carried out for 7 days. Simultaneously, samples without pretreatment straw and fermentation temperature of 35° C. were added as comparative examples.

[0055] 2. The result of straw degra...

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Abstract

The invention discloses a method for preparing hydrogen from straws, which comprises the following steps: carrying out aerobic degradation on straws by using a composite microbial inoculum capable of degrading straws; cattle manure pretreatment: screening the cattle manure with a 50-mesh screen to remove granular solids and impurities, boiling for 30-60 minutes, and centrifugating to remove excessive moisture for later use; preparation of straw anaerobic degradation culture medium: preparing a liquid anaerobic degradation culture medium, and adding the straws subjected to aerobic degradation; and adding the pretreated cattle manure into the straw anaerobic degradation culture medium, charging nitrogen into the container to remove oxygen, sealing, and culturing at 60-70 DEG C at the rate of 120 r / min in a dark place. The method optimally combines anaerobic degradation and anaerobic fermentation, has the advantages of short required time for hydrogen production, low cost, no need of adding enzymes in the fermentation process and cheap raw materials, and greatly saves the cost.

Description

technical field [0001] The invention relates to a method for preparing hydrogen by using straw, in particular to a method for preparing hydrogen by using straw to ferment biomass. Background technique [0002] my country is a large agricultural country in the world, with 1.5 billion mu of arable land. According to incomplete statistics, more than 700 million tons of straw will be produced after harvesting crops in my country every year. These straws are often of no value to be used. They were used as fuel or compost in the past. , kitchen electronic equipment into people's homes, and the widespread use of industrial fertilizers, straw is treated as garbage or incinerated. Therefore, a large amount of straw has brought us great environmental pressure, but it is also a cheap potential biomass energy. [0003] Straw is an organic carbohydrate, rich in C and H elements, and also contains a small amount of other organic matter such as protein. After microbial degradation, it can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P3/00C12R1/645C12R1/125C12R1/685C12R1/74
Inventor 周丽蓉崔泽周冯琳之
Owner 四川蓉加川大环保工程咨询设计有限公司
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