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Method for anaerobically degrading feather keratin with microbial strain 18D-TA

A feather keratin and microorganism technology, applied in the field of microorganisms, can solve the problems of increasing the time cost of industrial development, increasing the fermentation cost, large ventilation equipment, etc., and achieves the advantages of improving absorbability, short fermentation period and good substrate solubility. Effect

Active Publication Date: 2012-01-25
BIOGAS SCI RES INST MIN OF AGRI
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Problems solved by technology

This technology uses Stenotrophomonas maltophilia DHHJ to degrade hair keratin at 40°C for a long time of 48h-96h, which is likely to increase the time cost of industrial development. In addition, this bacteria belongs to aerobic bacteria degradation Hairy keratin requires larger aeration equipment, which increases the cost of fermentation; it is easy to cause bacterial contamination during aeration

Method used

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  • Method for anaerobically degrading feather keratin with microbial strain 18D-TA
  • Method for anaerobically degrading feather keratin with microbial strain 18D-TA

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Embodiment 1

[0027] The method for anaerobically degrading feather keratin using microbial strain 18D-TA is characterized in that the specific steps are as follows:

[0028] A. Feather treatment

[0029] After washing the feathers, put them in an oven at 45°C-55°C, and dry them for 2-3 days for later use;

[0030] B. Inoculation of strains

[0031] 1) Preparation of medium: Add 500ml of distilled water to a 1L Erlenmeyer flask in advance, add K 2 HPO 4 3 g / L, KH 2 PO 4 2.5 g / L, NaCl 3 g / L, MgSO 4 ·7H 2 O 0.02 g / L, CaCO 3 0.02 g / L vitamin solution 1% (v / v ), trace element solution 1% (v / v ) until dissolved, then add water to 1L, boil for 5-8min, cool to room temperature, add 0.1% (w / v v) Cysteine-HCl·H 2 O, adjust the pH to 7.0-7.5 with 5mol / L KOH, then add 0.1% (v / v) resazurin solution with a concentration of 0.1% (w / v), press 2.0m 3 / h pass N 2 , boiled for 15 minutes, cooled and divided into anaerobic tubes or serum bottles pre-added with 1% (w / v) feathers treated in step A, s...

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Abstract

The invention relates to a method for anaerobically degrading feather keratin with a microbial strain 18D-TA, belonging to the technical field of microorganisms. The method comprises the following specific steps of: A, treating feathers: cleaning feathers with water, putting into a drying oven of which the temperature is 45-55 DEG C, and drying for 2-3 days for later use; and B, inoculating the strain: performing high-temperature moist-heat sterilization on a culture medium and the feathers treated in the step A, inoculating the strain 18D-TA in the amount of 2-10 percent, and anaerobically standing and culturing at the temperature of 45-60 DEG C for 8-10 hours till feather down falls off, wherein the feathers are completely separated from feather stems 18-24 hours later, and the feather stems are degraded in different degrees. Compared with other aerobic bacteria, the strain used in the method has the advantages: more products can be accumulated, so that ventilation equipment is not required to be provided, and the energy consumption is lowered greatly; and meanwhile, the efficiency is high, the fermenting period is short, racemization of most amino acids is avoided, and the absorption applicability of a feather hydrolysis product is enhanced.

Description

technical field [0001] The invention relates to a method for degrading feather keratin, in particular to a method for anaerobically degrading feather keratin by using microbial strain 18D-TA, and belongs to the technical field of microorganisms. Background technique [0002] At present, the technology of transforming feather keratin resources mainly adopts physical and chemical methods that pollute, consume a lot of energy, and have low nutritional value. Biological methods, due to the high production cost of keratinase and the low efficiency of microbial degradation of feathers, affect the popularization and utilization of the technology. . For example, the patent application with application number 200810018317.2 discloses a method for preparing livestock and poultry hair keratin by ultrasonic treatment, including the steps of preparing keratin stock solution by reduction hydrolysis or oxidative hydrolysis, ultrasonic treatment and drying. This method requires reductive ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/10A23K1/16C12N1/20C12R1/01A23K10/12A23K10/26
Inventor 邓宇
Owner BIOGAS SCI RES INST MIN OF AGRI
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