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Method for enriching, domesticating and screening polycyclic aromatic hydrocarbon anaerobic degradation mixing flora and use thereof

A technology of polycyclic aromatic hydrocarbons and mixed flora, applied in the direction of bacteria, can solve the problems of high toxicity of microorganisms, refractory degradation, low water solubility of polycyclic aromatic hydrocarbons, etc.

Inactive Publication Date: 2009-05-27
BEIJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Li Xiaoming, Chen Jian, and Lun Shiyi's "rapid acclimation and enrichment technology of anaerobic microorganisms for degrading terephthalic acid" reported in the literature is the most relevant technology for this application, but due to polycyclic aromatic hydrocarbons, especially higher than four Polycyclic aromatic hydrocarbons are more difficult to be degraded by anaerobic biodegradation. At the same time, due to the low water solubility of polycyclic aromatic hydrocarbons and high toxicity to microorganisms, the domestication and enrichment technology of terephthalic acid anaerobic microorganisms cannot Adaptation Screening of Microbial Groups for Anaerobic Degradation of Polycyclic Aromatic Hydrocarbons

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The mixed flora domesticated by using the method of the present invention has carried out the anaerobic degradation test of polycyclic aromatic hydrocarbons under nitrate reduction conditions, and the results show that pure strains can effectively anaerobically degrade naphthalene, phenanthrene, fluorene and pyrene; when naphthalene When the initial concentration is 28.6mg / L, it can be degraded to below 0.05mg / L after 35 days. When the initial concentration of phenanthrene is 1.02mg / L, it can be degraded to below 0.05mg / L after 25 days. When the initial concentration of fluorene is 0.11 mg / L, it can be degraded to below 0.01mg / L after 20 days; when the initial concentration of pyrene is 0.12mg / L, it can be degraded to below 0.01mg / L after 20 days; anaerobic degradation of naphthalene, phenanthrene, fluorene and pyrene The rates are respectively (thalline concentration is 5×10 7 Individual / mL): 1.04mg·L -1 d -1 ;0.06mg·L -1 d -1 ;0.01mg·L -1 d -1 and 0.01mg·L -1 d...

Embodiment 2

[0018] Using the mixed flora domesticated by the method of the present invention to carry out the anaerobic degradation test of polycyclic aromatic hydrocarbons under sulfate reducing conditions, the results show that pure strains can effectively anaerobically degrade naphthalene, phenanthrene, fluorene and pyrene; when naphthalene When the initial concentration is 29.2mg / L, it can be degraded to below 0.05mg / L after 35 days. When the initial concentration of phenanthrene is 0.97mg / L, it can be degraded to below 0.05mg / L after 25 days. When the initial concentration of fluorene is 0.09 mg / L, it can be degraded to below 0.01mg / L after 20 days; when the initial concentration of pyrene is 0.11mg / L, it can be degraded to below 0.01mg / L after 20 days; anaerobic degradation of naphthalene, phenanthrene, fluorene and pyrene The rates are respectively (thalline concentration is 5×10 7 Individual / mL): 0.97mg·L -1 d -1 ;0.05mg·L -1 d -1 ;0.01mg·L -1 d -1 and 0.01mg·L -1 d -1 .

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Abstract

The invention belongs to the technical field of biodegradation treatment of contaminated soil and water, and discloses an enrichment, acclimation and screening method for an anaerobic degradation community of polycyclic aromatic hydrocarbon and application thereof. A mixture of an electron acceptor, sodium sulfide and the polycyclic aromatic hydrocarbon is added in the contaminated soil according to the mass ratio and is sealed and stood for 6 months, and then a mixed liquid of a basal culture medium, a micro metal liquid and a vitamin C solution is added into an anaerobic box full of nitrogen according to the proportion for soil acclimatization; the polycyclic aromatic hydrocarbon, the basal culture medium, the micro metal liquid, the vitamin C solution and an adsorbent are added into an anaerobic bottle in the anaerobic box full of nitrogen, the enriched and acclimatized soil is grafted into the anaerobic bottle according to the mass ratio of water to the soil, and the electron acceptor and the sodium sulfide are added according to the mass ratio. The soil is cultured for 20 to 25 days in a rotary oscillator with a temperature of between 18 and 22 DEG C and then is subjected to circular inoculation for more than 5 times to obtain a microbial community used for anaerobically degrading the polycyclic aromatic hydrocarbon. The method can enrich, acclimatize and screen the microbial community with good anaerobic degradation performance, and the degradation rate range of the polycyclic aromatic hydrocarbon is between 0.01 and 1.04mg.L<-1>.d<-1>.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, and in particular relates to a method for enrichment, domestication and screening of polycyclic aromatic hydrocarbon anaerobic degradation bacteria and its application. Background technique [0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of organic compounds with carcinogenic, teratogenic and mutagenic effects. They are widely distributed in the environment and have biodegradability, which has attracted widespread attention. The aerobic biodegradation of polycyclic aromatic hydrocarbons is the fastest, and many strains of aerobic degradation bacteria have been isolated and domesticated. However, underground aquifers or polluted soils are usually in an anaerobic state, so that aerobic microorganisms in the contaminated zone are replaced by anaerobic microorganisms. Therefore, anaerobic biodegradation of PAHs is more feasible and cost-effecti...

Claims

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Application Information

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IPC IPC(8): C12N1/20A62D3/02
Inventor 豆俊峰丁爱中
Owner BEIJING NORMAL UNIVERSITY
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