Method for improving biological coal bed gas yield through coal bed origin fungi

A bio-coalbed methane and coalbed methane technology, applied in the field of production increase and coalbed methane mining, can solve the problems of low methane production and rate of biodegradation coal, unsatisfactory field application conditions, etc., to solve the problem of methane production rate, output and adaptability strong effect

Active Publication Date: 2017-11-24
TAIYUAN UNIV OF TECH
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  • Application Information

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Problems solved by technology

However, due to the low yield and rate of methane produced by bio...

Method used

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  • Method for improving biological coal bed gas yield through coal bed origin fungi
  • Method for improving biological coal bed gas yield through coal bed origin fungi
  • Method for improving biological coal bed gas yield through coal bed origin fungi

Examples

Experimental program
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Effect test

Embodiment 1

[0032] Embodiment 1: the method for improving biological coalbed methane output

[0033] The specific steps of the method are as follows:

[0034] In the first step, the target CBM field is an area in the south of the CBM field in the Qinshui Basin9 # For coal seams, collect relevant geological data, collect coal samples from coalbed methane reservoirs, produce water samples at the wellhead of coalbed methane, and use Miseq high-throughput sequencing technology to analyze the microbial community structure of the collected water samples;

[0035] The results show that the reservoir pressure is 4.3Mpa and the reservoir temperature is 35°C. The archaeal groups of methanogens are mainly methanogens, the bacteria are mainly Proteobacteria and Firmicutes, and the fungi are mainly Ascomycota and Basidiomycota.

[0036] In the second step, using coal as the only carbon source, the microbial flora that degrades coal and produces methane is enriched from the produced water samples. T...

Embodiment 2

[0043] Embodiment 2: the method for improving bituminous coal bio-coal-bed methane output

[0044] The specific steps of the method are as follows:

[0045] In the first step, the target coal sample is a long-flame coal sample obtained from the northern area of ​​Qinshui Basin, and the source of the bacteria is the mixed bacteria group of fungi and methanogens enriched in Example 1.

[0046] In the second step, the target long-flame coal sample was used as the only carbon source to carry out the coalbed methane experiment of microbial degradation of coal products. The pH of the culture environment was 7.0, and the culture temperature was 35°C. The antibiotics added were ampicillin at a final concentration of 0.1 mM and streptomycin at a final concentration of 0.2 mM.

[0047] The third step is to cultivate under laboratory conditions for 30 days, and evaluate the gas production effect. The gas production of long-flame coal reaches 200 μmol / g coal, and the bacterial group sho...

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Abstract

The invention discloses a method for improving the biological coal bed gas yield through coal bed origin fungi. The method includes the steps that (1) target coal bed gas field data are collected, and a coal bed in-situ fungi community structure is analyzed; (2) origin fungus efficient flora cultivation is carried out; and (3) the coal bed gas production is increased through microorganisms. According to the method, the efficiency capacity of degrading macromolecular compounds through the fungi is used, origin fungus floras are cultivated from a coal bed, the microorganism increase production coal bed gas process of anaerobic degradation coal produced methane is achieved, the target coal bed environment can be better accommodated, coal bed in-site biological methane conversion is quickly achieved, the problems in biodegradation coal produced methane rate and yield are solved, the coal bed gas yield is improved, the coal bed gas well service life is prolonged, the microorganism increase production coal bed gas technical application is pushed, and the coal bed gas industrial development is promoted.

Description

technical field [0001] The invention belongs to the field of mining and increasing production of coalbed methane, and relates to a method for increasing the production of biological coalbed methane by using the original fungus of the coal seam, specifically a method of using the original fungus of the coalbed to anaerobically degrade coal to promote methane generation and increase the production of biological coalbed methane method. Background technique [0002] Coalbed methane is a new type of clean energy and an important alternative energy source in my country. It plays an important role in optimizing energy structure and improving energy supply. However, the low recovery rate of coalbed methane restricts the development of the coalbed methane industry. Microbial stimulation of coalbed methane takes advantage of the characteristics of microbial degradation of coal to produce methane, and realizes the regeneration of coalbed methane resources through the conversion of coa...

Claims

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Application Information

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IPC IPC(8): E21B43/22C12N1/14C12N1/20C12P5/02C12R1/01C12R1/645
CPCC12N1/14C12N1/20C12P5/023E21B43/006E21B43/16Y02E50/30
Inventor 郭红光韩青梁卫国
Owner TAIYUAN UNIV OF TECH
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