Preparation method of straw feed

A technology of straw feed and straw, which is applied in the field of preparation of straw feed, can solve the problems of increasing PHBV cost, environmental pollution, and increasing processing cost, and achieve the effects of reducing incineration, increasing nutritional value, and solving feed shortage

Inactive Publication Date: 2016-09-28
BEIJING UNION UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large amount of organic matter, direct discharge into the environment will cause serious environmental pollution problems
It can be treated by sewage treatment methods such as activated sludge, but it will increase the cost of PHBV
How to eliminate the pollution problem in PHBV production without increasing the processing cost, there is still no good solution

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) Cell culture

[0030] Use an inoculation loop to inoculate H.amylolyticum TNN58 into the seed medium, culture at 37°C, 220r / min, on a shaker for 24h, and then inoculate 120mL of the seed culture solution into a 7.5L fermenter containing 3L of fermentation medium (model Biotech -7BG-3). The fermentation conditions are: temperature 37°C, pH 7.0, stirring speed 400r / min, air flow speed 3vvm, fermentation time 180h.

[0031] Feed solution formula: glucose 100g / L, yeast extract 10g / L, NaCl 180g / L.

[0032] The feeding strategy is to regularly take samples to determine the concentration of residual sugar in the fermentation broth. When the residual sugar concentration is lower than 0.5g / L, supplement the glucose concentration to 10g / L, and the volume of supplemented feed liquid can be obtained by calculation.

[0033] (2) Supernatant preparation:

[0034]After the cell fermentation was completed, the cells were collected by centrifugation at 5000 r / min for 30 min. Th...

Embodiment 2

[0040] (1) Cell culture:

[0041] Inoculate H.amylolyticum TNN58 on the seed medium with an inoculation loop, culture at 37°C, 220r / min, on a shaker for 24h. Then, according to the inoculum amount of 5%, the seed culture solution was inoculated in the Erlenmeyer flask containing the fermentation medium at 37° C., 220 r / min, and cultured on a shaking table for 24 hours.

[0042] (2) Supernatant preparation:

[0043] After the cell fermentation was completed, the cells were collected by centrifugation at 5000 r / min for 30 min. Add the cells to 1 volume of 0.1% SDS aqueous solution, stir until the liquid becomes uniform, and the cells are completely lysed.

[0044] Centrifuge at 5000r / min for 30min. The precipitate was used for PHBV extraction, and the supernatant was used for straw feed preparation.

[0045] (3) Straw crushing:

[0046] The same amount of wheat straw and oat straw was crushed to a particle diameter of 1mm.

[0047] (4) supernatant mixed with straw:

[004...

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Abstract

The invention relates to a straw feed and a preparation method thereof. The preparation method comprises the following steps of culturing extremely halophilic archaea-halogranum amylolyticum TNN58; after the culturing of thalli is completed, performing cell pyrolysis by a water pyrolysis method. After centrifugal treatment, solids mainly comprise biological degradable plastics PHBV and part of intracellular substances, and precipitates can be used for PHBV extraction; supernatant contains a large quantity of nutrient substances, so that when the supernatant is added to straw, the content of nutrient substances such as protein in the straw can be effectively increased, and the nutrient value of the straw can be increased.

Description

technical field [0001] The invention belongs to the technical field of feed preparation, and in particular relates to an economical and environment-friendly preparation method of straw feed. Background technique [0002] my country's crop straw resources are very rich, with an annual output of more than 800 million tons. The main varieties of straw are corn, rice and wheat, of which corn straw occupies the largest amount. Straw is mainly composed of three substances: cellulose, hemicellulose and lignin. Low crude protein content, lack of vitamins, and low nutritional value. Therefore, straw has become the main agricultural waste. The waste and incineration of straw in the vast rural areas of our country is very serious, which not only causes a huge waste of resources, but also increases air pollution, making the smog weather worse in our country, which seriously violates the concept of sustainable development in our country. [0003] Straw mainly contains cellulose, hemic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/37A23K10/16C12N1/20C12R1/01
CPCC12N1/20Y02P60/87
Inventor 赵有玺马榴强冀颐之龚平程艳玲
Owner BEIJING UNION UNIVERSITY
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