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Method for increasing PHA yield by inhibiting halophilic archaea EPS yield

A technology for improving production and halophilic archaea, which is applied in the field of microbial fermentation to produce polymers, can solve the problems of high cost of recombinant strains, difficult engineering implementation, and complicated operation, so as to control fermentation costs, reduce sterilization costs, and simplify sterilization. The effect of the bacterial process

Inactive Publication Date: 2018-06-01
BEIJING UNIV OF TECH
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  • Claims
  • Application Information

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Problems solved by technology

However, the cost of recombinant strains is high, the operation is complicated, and it is not easy to realize engineering

Method used

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  • Method for increasing PHA yield by inhibiting halophilic archaea EPS yield

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Embodiment 1

[0037] In the embodiment, the strain used is Haloferax mediterranei, strain number ATCC 33500.

[0038] The seed medium of the strain contains: 7.5g of peptone per 1L of deionized water, 10g of yeast extract, 3g of sodium citrate, 20g of magnesium sulfate, 2g of potassium chloride, 0.01g of ferrous ion, and 200g of sodium chloride. And adjust the pH to 7.2.

[0039] The medium formula for fermentation is 200g of sodium chloride, 10g of glucose, 0.48g of ammonium chloride, 13g of magnesium chloride hexahydrate, 0.69g of calcium chloride dihydrate, 20g of magnesium sulfate heptahydrate, 4g of potassium chloride, and hydrogen carbonate per 1L of distilled water. Sodium 0.25g, sodium bromide 0.5g, potassium dihydrogen phosphate 0.439g, trace element solution 1ml (trace element solution is prepared by a known method, every 100ml of trace element solution contains zinc sulfate heptahydrate 0.1g, magnesium chloride tetrahydrate 0.03g, boric acid 0.3g, cobalt chloride hexahydrate 0.2...

Embodiment 2

[0046] In the embodiment, the strain used is Haloferax mediterranei, strain number ATCC 33500.

[0047] The seed medium of the strain contains: 7.5g of peptone per 1L of deionized water, 10g of yeast extract, 3g of sodium citrate, 20g of magnesium sulfate, 2g of potassium chloride, 0.01g of ferrous ion, and 200g of sodium chloride. And adjust the pH to 7.2.

[0048] The medium formula for fermentation is 250g of sodium chloride, 10g of glucose, 0.48g of ammonium chloride, 13g of magnesium chloride hexahydrate, 0.69g of calcium chloride dihydrate, 20g of magnesium sulfate heptahydrate, 4g of potassium chloride, and hydrogen carbonate per 1L of distilled water. Sodium 0.25g, sodium bromide 0.5g, potassium dihydrogen phosphate 0.439g, trace element solution 1ml (trace element solution is prepared by a known method, every 100ml of trace element solution contains zinc sulfate heptahydrate 0.1g, magnesium chloride tetrahydrate 0.03g, boric acid 0.3g, cobalt chloride hexahydrate 0.2...

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Abstract

The invention discloses a method for increasing PHA yield by inhibiting halophilic archaea EPS yield, and belongs to the field of producing polymer by microbial fermentation. According to the technical method, adjusting on concentration of NaCl in fermentation liquor can be used for inhibiting synthesis of EPS, so that propensity synthesis of PHA is promoted. When NaCl concentration in fermentation liquor is controlled to be 200g / l-250g / l, the yield of EPS can be inhibited, so that synthesis amount of PHA is remarkably improved. The technical method for synthesizing PHA by adjusting Haloferaxmediterranei propensity is simple to implement, and is easy for engineering operation.

Description

technical field [0001] The invention belongs to the field of microbial fermentation production of polymers, and relates to a technical method for product regulation during the fermentation production of polyhydroxyalkanoate (PHA) and extracellular polymer (EPS) by using the extreme halophilic archaea H. mediterranei. Background technique [0002] PHA is a general term for a class of polymers synthesized intracellularly by microorganisms. Because of its similar properties to petroleum-derived plastics, and its biocompatibility and biodegradability, it has become an ideal substitute for petroleum-derived plastics. In addition to being widely used in the packaging field, PHA can also be used in fields such as daily necessities and biomedical materials. [0003] The extreme halophilic archaea H. mediterranei has the ability to simultaneously synthesize PHA and EPS. However, the simultaneous synthesis of EPS and PHA leads to low carbon source utilization efficiency, which is no...

Claims

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Application Information

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IPC IPC(8): C12P7/62C12R1/01
CPCC12P7/625
Inventor 崔有为宫晓宇
Owner BEIJING UNIV OF TECH
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