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Enrichment culture method of ammonia-oxidizing archaea in sewage treatment system

A technology of ammonia oxidizing archaea and sewage treatment system, which is applied in the field of environmental microorganisms, can solve the problems that it is difficult to obtain pure ammonia oxidizing archaea enrichment culture solution, and the proportion of ammonia oxidizing archaea is small, so as to save power consumption, Strong competitiveness, the effect of improving the denitrification effect

Active Publication Date: 2013-12-18
HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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  • Abstract
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Problems solved by technology

[0005] In the traditional method of using domestic sewage or artificial water distribution to enrich and cultivate ammonia-oxidizing archaea, there will be a large number of heterotrophic bacteria in the enrichment culture system, and the proportion of ammonia-oxidizing archaea in the system is very small, so it is difficult to Obtain high purity ammonia oxidizing archaea enriched culture solution

Method used

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  • Enrichment culture method of ammonia-oxidizing archaea in sewage treatment system
  • Enrichment culture method of ammonia-oxidizing archaea in sewage treatment system
  • Enrichment culture method of ammonia-oxidizing archaea in sewage treatment system

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Enrichment culture method of ammonia oxidizing bacteria

[0034] (1) Preparation of ammonia oxidizing bacteria enrichment medium: NaCl 1g, MgCl 2 · 6H 2 O 0.4g, KCl 0.5g, CaCl 2 0.1g, KBr 0.1g, KH 2 PO 4 0.2g, water 1000mL. Sterilize at 121°C for 30 minutes, then add NH filtered through a 0.2 μm filter membrane 4 Cl (1M): 1 mL, NaHCO 3 Solution (1M): 2mL, EDTA Na-Fe solution: 1mL, sodium pyruvate solution (1M): 1mL, trace elements: 1mL, vitamins: 1mL, selenium-tungsten solution: 1mL.

[0035] (2) Enrichment culture of ammonia-oxidizing bacteria: inoculate the enrichment medium containing ammonia-oxidizing archaeal biofilm or activated sludge, adjust the pH value to 7.2-7.5, and incubator at a temperature of 37±1°C Cultured statically in medium, the culture time is 14-16 days as the first-generation culture cycle, cultured in a dark environment for 2 cycles, and supplemented with 1mL NH every 2-3 days 4 Cl solution and 2mL NaHCO 3 solution.

[0036] Purific...

Embodiment 2

[0040] Enrichment culture method of ammonia oxidizing bacteria

[0041] (1) Preparation of enriched medium: NaCl 2g, MgCl 2 · 6H 2 O 0.8g, KCl 1g, CaCl 2 0.2g, KBr 0.2g, KH 2 PO 4 0.4g, water 2000mL. Sterilize at 121°C for 30 minutes, then add NH filtered through a 0.2 μm filter membrane 4 Cl (1M): 2mL, NaHCO 3 Solution (1M): 4mL, EDTA Na-Fe solution: 2mL, sodium pyruvate solution (1M): 2mL, trace elements: 2mL, vitamins: 2mL, selenium-tungsten solution: 2mL.

[0042] (2) Enrichment culture of ammonia-oxidizing bacteria: inoculate the enrichment medium containing ammonia-oxidizing archaeal biofilm or activated sludge, adjust the pH value to 7.2-7.5, and incubator at a temperature of 37±1°C Cultured statically in medium, the culture time is 14-16 days as the first-generation culture cycle, cultured in a dark environment for 2 cycles, and supplemented with 2mL NH every 2-3 days 4 Cl solution and 4mL NaHCO 3 solution.

[0043] Purification and enrichment culture met...

Embodiment 3

[0047] Enrichment culture method of ammonia oxidizing bacteria

[0048] (1) Preparation of enrichment medium: NaCl 5g, MgCl 2 · 6H 2 O 2g, KCl 2.5g, CaCl 2 0.5g, KBr 0.5g, KH 2 PO 4 1g, water 5000mL. Sterilize at 121°C for 30 minutes, then add NH filtered through a 0.2 μm filter membrane 4 Cl (1M): 5mL, NaHCO 3 Solution (1M): 10mL, EDTA Na-Fe solution: 5mL, sodium pyruvate solution (1M): 5mL, trace elements: 5mL, vitamins: 5mL, selenium-tungsten solution: 5mL.

[0049] (2) Enrichment culture of ammonia oxidizing bacteria: inoculate biofilm or activated sludge containing ammonia oxidizing archaea in the enrichment medium, adjust the pH value to 7.2-7.5, and cultivate at a temperature of 37±1°C Cultivate statically in the box. The culture time is 14-16 days as the first-generation culture cycle, and the second-generation culture cycle is cultivated in a dark environment, and 5mL NH is supplemented every 2-3 days. 4 Cl solution and 10 mL NaHCO 3 solution.

[0050] P...

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Abstract

The invention provides an enrichment culture method of ammonia-oxidizing archaea in a sewage treatment system. The enrichment culture method comprises the following steps: A, preparing an enrichment culture medium of an ammonia-oxidizing bacteria flora; B, carrying out enrichment culture onto an ammonia-oxidizing bacteria flora, wherein a biological membrane or active sludge containing the ammonia-oxidizing archaea is inoculated in the enrichment culture medium; C, preparing an enrichment culture medium of the ammonia-oxidizing archaea, wherein antibiotics are added into the enrichment culture medium of the ammonia-oxidizing bacteria flora; and D, carrying out enrichment culture on the ammonia-oxidizing archaea, wherein a filter is used for filtering an enrichment culture solution and then transferring into the enrichment culture medium of the ammonia-oxidizing archaea in the step C to obtain the enrichment culture solution of the ammonia-oxidizing archaea with a higher purity. Possibility is provided for researching physiological and biochemical characteristics of the ammonia-oxidizing archaea by the enrichment culture solution of the ammonia-oxidizing archaea with the higher purity; the ammonia-oxidizing archaea has stronger tolerance; if the ammonia-oxidizing archaea is introduced to an ammonia-oxidizing enhancing technology, important practical significance is provided for improving biological denitrification capacity of the sewage treatment.

Description

technical field [0001] The invention relates to the field of environmental microorganisms, in particular to a method for enriching and culturing ammonia-oxidizing archaea for treating ammonia nitrogen in wastewater. Background technique [0002] Biological denitrification technology is one of the most important and commonly used technologies in water environment protection. The ammonia oxidation step in the nitrification process is the key step and rate-limiting step of biological denitrification technology. Research has long believed that the main microorganisms involved in this process are ammonia-oxidizing bacteria, but recent studies have found that an archaea also has the function of ammonia oxidation, called ammonia-oxidizing archaea. Although ammonia oxidizing bacteria play an important role in the process of ammonia oxidation in sewage treatment systems, it also has certain defects in biological nitrogen removal. The first is that the number of ammonia oxidizing bac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20
Inventor 金文标李志鑫岳洋洋梁昭云
Owner HARBIN INST OF TECH SHENZHEN GRADUATE SCHOOL
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