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Method for detecting heterotrophic bacteria content of industrial circulating water

A technology for industrial circulating water and heterotrophic bacteria, applied in the field of detection, can solve the problems of long measurement time, unfavorable monitoring of the water quality of circulating cooling water, etc. Effect

Inactive Publication Date: 2012-08-01
NANJING UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The advantage of this method is that it can reduce the error, but the measurement time is still very long, which is not conducive to timely monitoring of the water quality of the circulating cooling water
There is also a test paper developed by using this method of adding a chromogenic agent to the medium to determine the content of heterotrophic bacteria in the water sample, which improves the shortcomings of the standard plate counting method. Then take it out and put it in the incubator for 2 days. The operation is simple and it is widely used at present, but the measurement time is still very long

Method used

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  • Method for detecting heterotrophic bacteria content of industrial circulating water
  • Method for detecting heterotrophic bacteria content of industrial circulating water

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Prepare the reagents needed for the experiment:

[0025] Ninhydrin reagent: dissolve 0.6g fructose and 1.0g ninhydrin in 100ml distilled water, store in brown volumetric flask;

[0026] pH buffer preparation: pH=5.9, 45ml 1 / 15mol / 1 potassium dihydrogen phosphate solution and 5ml 1 / 15mol / l disodium hydrogen phosphate solution;

[0027] Accurately prepare 1.00mol / l NaOH solution and 1.00mol / l HCl solution according to the standard method;

[0028] The volume of circulating water is 100ml (the source of the water sample is the circulating cooling water used in the refinery)

[0029] a) The water sample is filtered, and the bacteria in the water sample are enriched on a microporous filter membrane made of nylon material with a filter membrane pore size of 0.22 μm;

[0030] b) Transfer the bacteria-enriched carrier to 5 ml of 1.00 mol / l NaOH solution, and heat in a water bath at 100°C for 50 minutes to obtain a hydrolyzate; this step causes the bacterial membrane to be cle...

Embodiment 2

[0035] Ninhydrin reagent: dissolve 0.6g fructose and 1.0g ninhydrin in 100ml distilled water, store in brown volumetric flask;

[0036] pH buffer preparation: pH=5.9, 45ml 1 / 15mol / l potassium dihydrogen phosphate solution and 5ml 1 / 15mol / l disodium hydrogen phosphate solution;

[0037] Accurately prepare 1.00mol / l NaOH solution and 1.00mol / l HCl solution according to the standard method;

[0038] It is 100ml that the volume of circulating water is taken (the water sample source is the circulating cooling water used by the refinery 7 days later than Example 1)

[0039] a) The water sample is filtered, and the bacteria in the water sample are enriched on the microporous filter membrane made of nylon and the filter membrane pore size is 0.45 μm;

[0040] b) Transfer the bacteria-enriched carrier to 5 ml of 1.00 mol / l NaOH solution, and heat it in a water bath at 95°C for 60 minutes to obtain a hydrolyzate; this step causes the bacterial membrane to crack and release protein, whi...

Embodiment 3

[0045] The drawing of the standard curve of "absorbance-heterotrophic bacteria number": take water samples of different volumes at the same interval, according to the determination steps of water samples, and refer to GB / T 14643.1-2009 to determine the plate count method of heterotrophic bacteria in water samples The heterotrophic bacteria of different volumes of water samples are measured, so that the number of heterotrophic bacteria in different volumes of water samples can be determined. The number of heterotrophic bacteria is used as the abscissa, and the absorbance value is the ordinate to draw a standard curve. The source of the circulating water sample is the same as in Example 1, specifically as follows :

[0046] Referring to the standard plate counting method in GB / T14643.1-2009, the method is as follows:

[0047] 1. Preparation of medium: beef extract: 3.0g, peptone: 10.0g, sodium chloride: 5.0g, agar: 15.0g, add about 950ml of water to the above reagents and heat t...

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Abstract

The invention discloses a method for detecting heterotrophic bacteria content of industrial circulating water. The method comprises the following steps of: intercepting and enriching bacteria in a water sample, cracking the enriched bacteria to release protein, hydrolyzing the bacteria into amino acid, performing reactive color development on the amino acid by using ninhydrin, and comparing the measured absorbance with an 'absorbance-heterotrophic bacteria quantity' standard curve to obtain the quantity of the heterotrophic bacteria in the industrial circulating water with certain volume. The measuring method is quick and convenient compared with a national standard plate counting method, the heterotrophic bacteria content of the circulating cooling water can be acquired in short time, and the method facilitates instrumentation and automation and is easy to popularize.

Description

technical field [0001] The invention belongs to the technical field of detection, and relates to a detection method for the content of heterotrophic bacteria in industrial circulating water. Background technique [0002] Heterotrophic bacteria refer to a type of mixed bacteria that rely on organic matter nutrition as a carbon source for the synthesis of their own bacteria, and rely on the oxidation of organic matter to produce chemical energy for metabolism. In industrial circulating cooling water, heterotrophic bacteria not only grow and reproduce the fastest, but also have the largest number, which basically represents the number of all bacteria in the water. Therefore, in general measurement, the number of heterotrophic bacteria is often used to represent the total number of bacteria in the water. This type of flora can produce dense mucus, adhere to fine suspended matter in water and other filamentous bacteria, molds, algae, protozoa, etc., thereby forming slime. The ac...

Claims

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Application Information

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IPC IPC(8): C12Q1/06G01N21/31
Inventor 陈国松魏寒桥张琳昀唐美华张之翼
Owner NANJING UNIV OF TECH
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