49 results about "Bio distribution" patented technology

The invention discloses a radionuclide-labeled collagen hybrid peptide probe as well as a preparation method and application thereof. The chemical structural general formula of the radionuclide-labeled collagen hybrid peptide probe is (metal nuclide-chelating agent)-Linker-CHP, wherein the metal nuclide is selected from any one of Al18F, 64Cu, 67Cu, 67Ga, 68Ga, 99mTc, 89Zr, 111In, 177Lu, 186Re and 225Ac, and the metal nuclide is selected from any one of Al18F, 64Cu, 67Cu, 67Ga, 68Ga, 99mTc, 89Zr, 111In, the chelating agent is selected from any one of NODAGA, NOTA2 and DOTA; linker is a connecting joint, and CHP is a collagen hybrid peptide with a polypeptide sequence of (GfO) n or (GPO) n. The probe not only has the characteristic of radiochemical marking stability, but also has the excellent biological distribution characteristics of high uptake in target organs such as tumors and low uptake in non-target organs such as liver and kidney, and has a good clinical application prospect.

The invention discloses a microbial spatial distribution monitoring method and system based on a multi-level microscopic scanning image, relates to the technical field of image processing, and is used for carrying out multi-level grid traversal type scanning image acquisition on an observation area of microscopic examination data. Identifying and counting the number of each target microorganism in each image and the position information of each target microorganism in the corresponding hierarchical grid, and storing the information into a to-be-identified hierarchical data set corresponding to each target microorganism; and according to the ecological type of each target microorganism, respectively inputting the corresponding to-be-identified hierarchical data set into the corresponding trained multi-organism distribution probability density model to obtain a spatial distribution rule of each microorganism in the microscopic examination data. By adopting multi-stage magnification grading scanning, the interaction and distribution rules among microbial species are respectively described according to microbial ecological types, and the accuracy of microbial distribution observation is improved.

The application discloses a kind of aquatic habitat positioning prediction methods, it is related to aquatic habitat distribution research field.The present application establishes the aquatic habitat positioning framework of suitable area + suitable river reach by integrating the target biological distribution point and the environmental condition of river reach in target area.The fine prediction of aquatic habitat is realized in the multi-scale of area plus river reach, when facing the need of target biological habitat prediction in wide range area, fine positioning can be realized, so as to help fine management of aquatic organism, which has important guiding significance and practical significance for maintaining ecosystem stability.The common characteristics of suitable river reach are obtained by comparing after predicting suitable river reach, so as to identify and judge the potential habitat of target organism, the identification of potential habitat can expand the positioning range of target biological habitat, which can effectively avoid habitat omission and protection in time.

The invention provides a dynamic tumor segmentation method suitable for BNCT treatment and a model training method. The dynamic tumor segmentation method comprises the following steps: acquiring PET-CT multi-modal data; inputting the PET-CT multi-modal data into a trained tumor segmentation model, so as to obtain a tumor segmentation probability graph based on a spatial position dimension and a boron concentration distribution dimension according to the tumor segmentation model; and performing dynamic threshold segmentation on the tumor segmentation probability graph based on boron drug metabolism kinetic characteristics to obtain a tumor segmentation result. According to the method, the PET-CT multi-mode data, the boron sensitive attention mechanism and the BNCT boron drug metabolism kinetic characteristics are combined, high-precision segmentation of the tumor is achieved, the segmentation result reflects the boron drug biological distribution characteristics at the same time, and more accurate input parameters are provided for a BNCT treatment plan.

This invention relates to a self-adjuvanted biomimetic lipid nanoparticle, its microfluidic assembly method, and its anti-tumor applications. The self-adjuvanted biomimetic lipid nanoparticle has a structure in which an activated dendritic cell membrane (ADCM) is coated on the surface of a lipid nanoparticle (LNP) loaded with mRNA. The advancements of this invention compared to traditional LNPs are: the self-adjuvanted biomimetic lipid nanoparticle (ADCM-LNP) exhibits a faster cellular uptake rate and higher transfection efficiency, thereby inducing a strong Th1-type immune response and a potent CTL-mediated tumor-killing effect. In vivo biodistribution studies have shown that this system exhibits significant spleen-targeting (splenic tropism) and demonstrates excellent therapeutic efficacy in a HER2-positive breast cancer model.

[Problem] To provide a denitrification filter material in which the form thereof is maintained without collapsing and which is digested only when fed on by denitrifying bacteria. [Solution] A denitrification filter material according to the present invention is configured from a biodegradable melt-kneaded resin composition containing (A) a water-insoluble and / or non-seawater-soluble biodegradable resin component and (B) a biomass polysaccharide polymer component. The component (A) is preferably an ester-bonded polymer, and is more preferably one or more selected from polybutylene succinate adipate, polyhydroxyalkanoate, and polycaprolactone. The component (B) preferably contains a biomass polysaccharide polymer component having a helical molecular structure. A water purification device comprises: a denitrification tank to which water being treated is supplied; the aforementioned denitrification filter material, which is accommodated in the denitrification tank and fixes aerobic denitrifying bacteria for reducing nitrate nitrogen in the water being treated; and an air supply mechanism that supplies air to the water being treated.

The present disclosure discloses a brand-new skeleton 99mTc-FAPI diagnostic probe and use thereof in the preparation of a drug or a reagent for diagnosing tumors, wherein the structural formula of a dimeric compound targeting FAP is shown by following formula. Compared with conventional FAP inhibitor radiopharmaceuticals, technetium-99m-labeled novel skeleton FAP inhibitor dimeric compound has a very high tumor uptake rate, high contrast between tumor and background and good in vivo biodistribution.

The invention discloses a sparse scanning path planning method and system based on a multi-magnification microscopic image, and relates to the technical field of microscopic microbe quantity statistics, low-magnification scanning is performed on a to-be-detected sample observation area, and a zoogloea distribution high-density area is screened out from an obtained zoogloea distribution image; calculating the global probability density of each high-density area by using a zoogloea dependent biological distribution model, determining a medium-magnification scanning path, and inputting the obtained medium-magnification microscopic image into the zoogloea dependent biological distribution model again to obtain a first total number of target organisms; and determining a high-magnification scanning path according to the distribution sequence of the first target organisms in the medium-magnification microscopic image, and inputting the obtained high-magnification microscopic image into the constructed biological association type distribution model to obtain the total number of the second target organisms. Based on a distribution association rule among microorganisms, a layered scanning strategy of locking a high-density area is adopted, so that the scanning amount and the empty scanning rate are greatly reduced.

The invention relates to the field of nuclear medicine, in particular to an imaging probe based on radionuclide labeling and application. The imaging probe based on radionuclide labeling provided by the invention comprises lanthanide nanocrystals, an amphiphilic block copolymer self-assembled by the lanthanide nanocrystals, and radionuclide ions labeled on the nanocrystals, and the imaging probe simultaneously realizes time-gated intensity imaging, life imaging and SPECT (Single Photon Emission Computed Tomography) imaging. The imaging probe based on radionuclide labeling is provided for the first time, the radionuclide labeling rate as high as 99.6% is achieved, the imaging probe not only supports time gating intensity imaging with high signal-to-background ratio, but also supports life imaging and SPECT imaging, and in-vivo biological distribution and metabolism tracking of radioactive nanoparticles are achieved.

The application discloses a microorganism spatial distribution monitoring method and system based on multi-level microscopic scanning images, relates to the field of image processing, and adopts multi-level grid traversal scanning image collection on the observation area of microscopic data, identifies and counts the number of each target microorganism in each image and the position information of each target microorganism in the corresponding level grid, and stores each target microorganism in the corresponding to-be-identified level data set; according to the ecological type of each target microorganism, the corresponding to-be-identified level data set is respectively input into the corresponding trained multi-organism distribution probability density model, and the spatial distribution law of each type of microorganism in the microscopic data is obtained. The multi-level magnification grading scanning is adopted, the interspecific interaction and distribution law of microorganisms are respectively described according to the ecological type of microorganisms, and the observation accuracy of the distribution of microorganisms is improved.

The present disclosure provides a modified AAV capsid protein comprising a targeting peptide in variable region VIII (VR, VIII) and / or a peptide segment in variable region I (VR I). The modified AAV capsid protein can form an rAAV, which has a preferred tropism, specificity or biodistribution in vivo or in vitro. The rAAV of the present disclosure can be used for gene therapies targeted at a specific tissue.

The present disclosure discloses a brand-new skeleton 99mTc-FAPI diagnostic probe and use thereof in the preparation of a drug or a reagent for diagnosing tumors, wherein the structural formula of a dimeric compound targeting FAP is shown by following formula. Compared with conventional FAP inhibitor radiopharmaceuticals, technetium-99m-labeled novel skeleton FAP inhibitor dimeric compound has a very high tumor uptake rate, high contrast between tumor and background and good in vivo biodistribution.

The present disclosure provides a modified AAV capsid protein comprising a targeting peptide in a variable region VIII (VRVIII). The modified AAV capsid protein may form an rAAV having a preferred tropism, specificity, or biological distribution in vivo or in vitro. The rAAVs of the present disclosure can be used in gene therapy targeting specific tissues.

The invention discloses a brand new carrier-based siRNA drug targeting delivery method, and belongs to the technical field of biological medicine, the delivery system comprises: a core layer: nanoparticles formed by loading siRNA on a biodegradable polymer (such as PLGA, molecular weight of 10,000-100,000 Da); the middle layer is a pH sensitive polymer (such as PDEAEMA) coating the core layer and is triggered to release in a target tissue acidic microenvironment (pH is less than or equal to 6.5); and the outer layer is a targeting ligand (such as an anti-EGFR / c-Met antibody fragment, the connection density is 1-10 / nm), and specific enrichment is realized. Through the design of the intelligent responsive nano-carrier, the problem of biological distribution heterogeneity is effectively solved, the delivery efficiency of siRNA drugs in target tissues of different patients is improved, the treatment effect is more stable and reliable, and the application prospect is wide. The nano-carrier is constructed by adopting a biodegradable material with low immunogenicity, so that the drug resistance risk is remarkably reduced, the in-vivo circulation time of the carrier is prolonged, and a guarantee is provided for long-term treatment of siRNA drugs.

Provided is a universal preclinical bio-distribution detection kit for NK cell therapy products. The present invention further relates to specific use of a gene sequence set forth in SEQ ID No. 1 in distinguishing a human NK cell gene from a non-human animal gene. By means of providing a specific DNA sequence, a DNA sequence derived from the human NK cell can be specifically distinguished from a DNA sequence derived from the non-human animal gene, and based on the specific DNA sequence, a qPCR system for distinguishing the human NK cell gene from the non-human animal gene is constructed, providing a primer pair and a probe set for NK cells and derivative cell therapy products thereof. In addition, a universal preclinical bio-distribution detection kit for the NK cell therapy products is successfully designed, thereby providing convenience for the preclinical research on the NK cells and derivative cell therapy products thereof.

A monitoring apparatus (10) for aquatic organisms, which monitors the distribution of aquatic organisms within a monitored water area. The monitoring apparatus (10) comprises: an acoustic sensor (1), which emits an ultrasonic beam into water and receives reflected wave signals from aquatic organisms; an aquatic organism counting unit (2), which counts the aquatic organisms within a detection range of the acoustic sensor on the basis of the reflected wave signals; a detection region area calculation unit (3), which calculates a beam width at a predetermined depth on the basis of an extension angle of the ultrasonic beam, and calculates the area of a detection region at the predetermined depth on the basis of the beam width; and a living density calculation unit (4), which calculates, on the basis of the number of aquatic organisms counted and the area of the detection region, a density distribution of the aquatic organisms within the range of the monitored water area.

The present invention relates to methods for large tissue labeling, clearing and / or imaging using labeling agents such as antibodies, and uses and products related thereto. The present invention includes inter alia methods for preparing an animal tissue for fluorescence microscopy, an animal tissue obtainable by said methods, methods for analyzing said animal tissues, and methods for the detection of metastases, for analyzing the biodistribution of a biopharmaceutical drug, and for analyzing the biodistribution of nanoparticles. The methods for preparing an animal tissue according to the present invention encompass whole-body labeling, clearing and imaging methods. The methods of the invention are advantageous in that they, for instance, allow the visualization of single cells within mammalian tissues, including whole mouse bodies or other large tissues, tumor metastases at the single cell level and of the distribution of biopharmaceutical drugs (e.g. the distribution of cancer-targeting therapeutic antibodies in whole animals such as intact mice) at single cell level in whole mouse using labeling agents such as antibodies.

The invention discloses a ship ultraviolet light organism adhesion prevention device and method fused with visual analysis, and relates to the technical field of ship antifouling, and the method comprises the steps: obtaining the type of an organism adhered to a ship body; a target frequency corresponding to the biological type is obtained, the action range of the ultraviolet lamp set at the installation position is obtained through analysis, and the part, below the water surface, of the ship body is divided into at least one local area; analyzing to obtain the real-time seawater temperature of the local area, analyzing to obtain the real-time seawater flow velocity and the biological distribution condition of the local area, and setting the operation frequency of the ultraviolet lamp group corresponding to the local area; and establishing an ultraviolet lamp intensity loss model, and setting the operation intensity of the ultraviolet lamp group corresponding to the local area. The biological distribution condition, the real-time seawater temperature and the real-time seawater flow velocity are obtained in an image analysis mode, and the established ultraviolet lamp intensity loss model is combined, so that the illumination setting is consistent with the actual condition as much as possible, the energy efficiency is improved, and the illumination mode is optimized.

A bran-new skeleton 99mTc-FAPI diagnostic probe and use thereof in preparation of drugs or reagents for diagnosing tumors are provided. A fibroblast activation protein-α (FAP)-targeting dimer compound has a structure represented by the following formula. Relative to the traditional FAP inhibitor radioactive drug, a technetium-99m labeled new skeleton FAP inhibitor dimer compound has an extremely high tumor intake rate, and has a relatively high contrast between the tumor and a background and has a good in-vivo biological distribution.

The invention discloses a nuclide-labeled targeted PET probe as well as a preparation method and application thereof, and provides a targeted reagent which comprises a PARP inhibitor, a fluorosulfonate group modified unnatural amino acid and an imaging agent, the structural formula of the targeting reagent is as shown in formula I in the specification. The targeting reagent provided by the invention has high radiochemical purity, good in-vitro stability and moderate Log D7.4. Small animal PET imaging and biological distribution research show that the polypeptide can be quickly cleared from normal tissues and is mainly excreted through a urinary system, obvious uptake of PARP-1 positive tumors is shown, the residence time of the tumors is prolonged, the signal-to-noise ratio of the tumors to the normal tissues is high, and the polypeptide has the potential of serving as a PARP-1 expression tumor PET diagnostic agent.

This invention discloses a 99mTc-FAPI diagnostic probe having a novel skeleton and its application in the manufacture of a tumor diagnostic drug or reagent, the structural formula of the FAP-targeting dimer compound is shown in the following formula. Compared to conventional FAP inhibitor radiopharmaceuticals, the technetium-99m-labeled FAP inhibitor dimer compound having a novel skeleton exhibits a very high tumor uptake rate, high contrast between tumor and background, and good in vivo biological distribution. [C28] TIFF2026518096000031.tif51170

Disclosed herein are compositions and methods for deploying interventional agents into constrained biological states using non-crystalline amorphous iron oxide nanoparticles (NAIONPs). The NAIONPs comprise coordination-stabilized, aqueous colloidal dispersions lacking long-range crystalline order and presenting surface-accessible coordination sites for reversible or covalent association with one or more interventional agents. The compositions are configured to modify the biological context in which an interventional agent is presented, enabling functional engagement of agents whose deployment is limited when administered in isolation due to factors including impaired biodistribution, reduced stability, stress sensitivity, immune-mediated clearance, altered cellular responsiveness, or non-optimal exposure conditions. Interventional agents may include polynucleotides, peptides, proteins, small molecules, targeting moieties, radiologic agents, or combinations thereof. The disclosed compositions and methods are applicable to in vivo, ex vivo, and in vitro biological systems, including oncologic disease states such as primary tumors, metastatic disease, hematologic malignancies, and treatment-refractory cancers.

Disclosed in the present invention are a novel scaffold 99mTc-FAPI diagnostic probe and a use thereof in preparation of a drug or a reagent for diagnosing a tumor. The structural formula of a dimeric compound targeting FAP is shown as follows. Compared with a conventional FAP inhibitor radiopharmaceutical, the technetium-99m-labeled novel scaffold FAP inhibitor dimeric compound has a very high tumor uptake rate, and exhibits high tumor-to-background contrast and good in vivo biodistribution.

The present invention provides a modified AAV capsid protein comprising a targeting peptide within variable region VIII (VR VIII) and / or a peptide fragment within variable region I (VR I). The modified AAV capsid proteins may form rAAVs having a specific tropism, specificity, or biodistribution in vivo or in vitro. The rAAV of the invention can be used for gene therapy for specific tissues.

The invention relates to a radiolabeled Exendin-4 polypeptide probe precursor and application thereof, and belongs to the technical field of radiopharmaceutical chemistry and nuclear medicine diagnosis and treatment. The radioactive labeled Exendin-4 polypeptide probe precursor is R-HBED-CC-PEGn-Exendin-4, the general formula structure of the radioactive labeled Exendin-4 polypeptide probe precursor is shown in the specification, and n is equal to 3, 6 or 12; r is-OH or PEG chains with different lengths are introduced into R-HBED-CC-Exendin-4 to modify the medicine, so that the pharmacokinetics of the medicine is changed. Through a radionuclide labeling experiment, an in-vitro cell uptake experiment, an in-vitro receptor competitive binding experiment, biological distribution, model mouse imaging and other experiments, the targeting and in-vivo metabolism level of the novel probe are explored. And a novel targeted GLP-1R diagnosis and treatment drug with a clinical application prospect is screened out.

The invention provides an environment restoration method based on a coral transplantation technology, and relates to the technical field of marine ecological environment protection. The method comprises the following steps: firstly, collecting benthic organism samples through marine ecological environment investigation, recording distribution characteristics, and generating benthic organism distribution data; on the basis, analyzing a biocenosis structure and evaluating ecological sensitivity, determining a coral transplantation type list and transplantation region coordinates, and generating a coral transplantation plan; then constructing a coral-microorganism symbiotic system in a transplantation area by utilizing a microbiome enhanced transplantation technology, and generating three-dimensional coordinate data of the symbiotic system; and finally, combining an engineering construction time sequence and coordinate data of the symbiotic system, arranging a suspended matter prevention and control device to limit pollutants from being migrated to the symbiotic area. According to the method, the survival rate of corals is increased through microbial enhancement, diffusion of suspended solids is accurately prevented and controlled, and collaborative optimization of engineering disturbance minimization and ecological restoration in the abrupt-change terrain area is achieved.

The present invention relates to methods for labeling, clearing, and / or imaging large tissues using labeling agents such as antibodies, and related uses and products. The present invention includes, inter alia, methods for preparing animal tissues for fluorescence microscopy, animal tissues obtainable by the methods, methods for analyzing the animal tissues, and methods for detecting metastasis, analyzing the biodistribution of biopharmaceuticals, and analyzing the biodistribution of nanoparticles. Methods for preparing the animal tissues according to the present invention include whole-body labeling, clearing, and imaging methods. The methods of the present invention are advantageous in that they allow visualization of, for example, single cells within mammalian tissues, including whole mice or other large tissues, tumor metastasis at the single-cell level, and the distribution of biopharmaceuticals at the single-cell level in whole mice using labeling agents such as antibodies (e.g., the distribution of cancer-targeting therapeutic antibodies in whole animals, such as intact mice).

The invention provides a sand or dust parameterization scheme construction method considering biological soil crust, and belongs to the field of sand and dust numerical simulation, the method comprises the following steps: based on survey sampling data of a research area, obtaining a biological suitable region and a biological distribution region, and intersecting the biological distribution region and the biological suitable region to obtain a crust distribution region; performing grid division on the research area, and obtaining the soil crust coverage rate of each organism according to the crust distribution area in each grid; constructing a calculation formula of the critical friction wind speed growth rate, and calculating the critical friction wind speed growth rate in combination with the soil crust coverage rate of each organism; and constructing a soil crust sand and dust emission formula, and calculating the sand and dust emission amount according to the critical friction wind speed growth rate to complete construction of a sand raising or dust raising parameterization scheme. The problems that an existing sand raising scheme does not consider the influence of biological earth surface crust on the sand raising process and the accuracy and reliability of sand raising or dust raising simulation of a land parcel with biological soil crust are low are solved.

The invention relates to the technical field of production quality monitoring, and particularly discloses an intelligent quality monitoring method for xylitol production, which comprises the following steps: S1, acquiring target microorganisms and environmental indexes and ranges required by growth of the target microorganisms, performing rectangular grid division on a contact surface of a xylitol production workshop, and acquiring regional environmental indexes in real time; s2, determining environment conformity according to the regional environment index value and the index range required by the target organism, correcting an initial detection period, setting a detection point, and obtaining an organism density value according to the correction period; s3, area points and biological density values are obtained, a density value gradient curve and a gradient center are obtained, a target biological distribution area is determined, a warning area is selected on a contact surface, and workshop disinfection is prompted when the distribution area and the warning area intersect; by acquiring regional environment indexes and biological density values in real time, the growth condition of microorganisms and environment change can be mastered in time, and the hysteresis problem of a traditional microorganism culture method is solved.