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Composite nano-drug carrier as well as preparation method and application thereof

A nanoparticle and system technology, applied in the field of biomedicine

Pending Publication Date: 2022-03-01
QIQIHAR MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the efficacy of drugs is still limited by some limitations of drug carriers, such as leakage of chemotherapeutic agents, uptake of chemotherapeutic agents by RES organs, insufficient penetration and accumulation of nanomedicines in tumor sites through the EPR effect.

Method used

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  • Composite nano-drug carrier as well as preparation method and application thereof
  • Composite nano-drug carrier as well as preparation method and application thereof
  • Composite nano-drug carrier as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0103] Embodiment 1: the investigation of PSA molecular weight

[0104] 1. Preparation of nanoparticles

[0105] Use PSA of different molecular weights to prepare PSA-zein nanoparticles respectively, and the specific steps are as follows:

[0106] Zein (100 mg) was dissolved in 5.0 mL ethanol / water (85:15, v / v). After stirring at 1000rpm for 1 hour, the above mixed solution was adjusted to pH 5.7 with 1mol / L HCl, and was added dropwise to 20.0mL 0.05% Tween 80 solution (adjusted to pH 4.0) under magnetic stirring (1000rpm) for 30min . Then, the remaining ethanol was removed by rotary evaporator at 37 °C.

[0107] For the method of coating zein nanoparticles with PSA, the resulting zein nanoparticle dispersion (5.0 mL) was injected into 5.0 mL of PSA solution (adjusted to pH 4.0) with constant stirring (1000 rpm) for 1 h . The prepared samples were centrifuged at 12000 rpm to remove impurities. The resulting samples were zein / PSA core-shell nanoparticles and were referred...

Embodiment 2

[0112] Embodiment 2: the investigation of PSA solution concentration

[0113] 1. Preparation of nanoparticles

[0114] PSA-zein nanoparticles were prepared using different concentrations of PSA solutions, and the specific steps were as follows:

[0115] Zein (100 mg) was dissolved in 5.0 mL ethanol / water (85:15, v / v). After stirring at 1000rpm for 1 hour, the above mixed solution was adjusted to pH 5.7 with 1mol / L HCl, and was added dropwise to 20.0mL 0.05% Tween 80 solution (adjusted to pH 4.0) under magnetic stirring (1000rpm) for 30min . Then, the remaining ethanol was removed by rotary evaporator at 37 °C.

[0116] For the method of coating zein nanoparticles with PSA, the resulting zein nanoparticle dispersion (5.0 mL) was injected into 5.0 mL of PSA solution (adjusted to pH 4.0) with constant stirring (1000 rpm) for 1 h . The prepared samples were centrifuged at 12000 rpm to remove impurities. The resulting samples were zein / PSA core-shell nanoparticles and were re...

Embodiment 3

[0121] Example 3: Preparation and Characterization of Zein / PSA Nanoparticles Loaded with HNK

[0122] 1. Preparation of nanoparticles

[0123] Zein (100 mg) and HNK (10 mg) were dissolved in 5.0 mL of ethanol / water (85:15, v / v). After stirring at 1000rpm for 1 hour, the above mixed solution was adjusted to pH 5.7 with 1mol / L HCl, and was added dropwise to 20.0mL 0.05% Tween 80 solution (adjusted to pH 4.0) under magnetic stirring (1000rpm) for 30min . Then, the remaining ethanol was removed by rotary evaporator at 37 °C.

[0124] For the method of coating zein-HNK nanoparticles with PSA, the resulting zein-HNK nanoparticle dispersion (5.0 mL) was injected into 5.0 mL of 0.1% PSA (PSA molecular weight 80 kDa) (adjusted to pH 4.0) in the solution, keep stirring (1000rpm) for 1 hour. Freshly prepared samples were centrifuged at 12000 rpm to remove unencapsulated HNK. The resulting sample is HNK-loaded zein / PSA core-shell nanoparticles and is called PSA-zein-HNK (abbreviated as...

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Abstract

The invention discloses a composite nano-drug carrier as well as a preparation method and application thereof, and particularly relates to a polysialic acid (PSA) modified zein nano-drug carrier and a drug loading system thereof, in particular to a tumor targeted drug delivery system, and preparation methods and application of the polysialic acid (PSA) modified zein nano-drug carrier and the drug loading system of the polysialic acid (PSA) modified zein nano-drug carrier and the tumor targeted drug delivery system. The drug carrier can realize enhanced drug delivery efficiency, active targeting ability and specific biological distribution at tumor sites, thereby inhibiting growth, migration and invasion of tumors. By taking HNK as an example, experiments prove that the PSA-zein-HNK has enhanced tumor accumulation in a mouse model carrying 4T1 breast cancer, so that an ideal anti-tumor effect and favorable biological safety are generated, and moreover, the PSA-zein-HNK remarkably inhibits the breast cancer from being transferred to the lung and the liver. Therefore, the nanoparticles are expected to become an effective tumor targeting drug carrier for drug development and disease treatment.

Description

technical field [0001] The present invention relates to the technical field of biomedicine, in particular to a composite nano-drug carrier and its preparation method and application, in particular to a polysialic acid (PSA)-modified zein nano-drug carrier and its drug-loading system, especially The invention relates to a tumor-targeted drug delivery system, as well as the preparation method and application of the two. Background technique [0002] With the development of society and economy and the improvement of people's living standards, affected by factors such as changes in dietary structure, population aging, and urbanization, the spectrum of diseases and deaths has changed significantly worldwide, and chronic non-communicable diseases have become the main cause of death. reason. Among them, malignant tumor is one of the main causes of death in the world at present, and has become a large class of diseases that seriously endanger human life and health and restrict soci...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/36A61K47/42A61K31/05A61P35/00A61P37/02A61P31/00B82Y5/00B82Y40/00A61K47/69A61K47/61
CPCA61K9/5161A61K47/42A61K31/05A61P35/00A61P37/02A61P31/00B82Y5/00B82Y40/00A61K47/6931A61K47/61
Inventor 蔡德富张琪王婧刘丹
Owner QIQIHAR MEDICAL UNIVERSITY
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