Composite nano-drug carrier as well as preparation method and application thereof
A nanoparticle and system technology, applied in the field of biomedicine
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Embodiment 1
[0103] Embodiment 1: the investigation of PSA molecular weight
[0104] 1. Preparation of nanoparticles
[0105] Use PSA of different molecular weights to prepare PSA-zein nanoparticles respectively, and the specific steps are as follows:
[0106] Zein (100 mg) was dissolved in 5.0 mL ethanol / water (85:15, v / v). After stirring at 1000rpm for 1 hour, the above mixed solution was adjusted to pH 5.7 with 1mol / L HCl, and was added dropwise to 20.0mL 0.05% Tween 80 solution (adjusted to pH 4.0) under magnetic stirring (1000rpm) for 30min . Then, the remaining ethanol was removed by rotary evaporator at 37 °C.
[0107] For the method of coating zein nanoparticles with PSA, the resulting zein nanoparticle dispersion (5.0 mL) was injected into 5.0 mL of PSA solution (adjusted to pH 4.0) with constant stirring (1000 rpm) for 1 h . The prepared samples were centrifuged at 12000 rpm to remove impurities. The resulting samples were zein / PSA core-shell nanoparticles and were referred...
Embodiment 2
[0112] Embodiment 2: the investigation of PSA solution concentration
[0113] 1. Preparation of nanoparticles
[0114] PSA-zein nanoparticles were prepared using different concentrations of PSA solutions, and the specific steps were as follows:
[0115] Zein (100 mg) was dissolved in 5.0 mL ethanol / water (85:15, v / v). After stirring at 1000rpm for 1 hour, the above mixed solution was adjusted to pH 5.7 with 1mol / L HCl, and was added dropwise to 20.0mL 0.05% Tween 80 solution (adjusted to pH 4.0) under magnetic stirring (1000rpm) for 30min . Then, the remaining ethanol was removed by rotary evaporator at 37 °C.
[0116] For the method of coating zein nanoparticles with PSA, the resulting zein nanoparticle dispersion (5.0 mL) was injected into 5.0 mL of PSA solution (adjusted to pH 4.0) with constant stirring (1000 rpm) for 1 h . The prepared samples were centrifuged at 12000 rpm to remove impurities. The resulting samples were zein / PSA core-shell nanoparticles and were re...
Embodiment 3
[0121] Example 3: Preparation and Characterization of Zein / PSA Nanoparticles Loaded with HNK
[0122] 1. Preparation of nanoparticles
[0123] Zein (100 mg) and HNK (10 mg) were dissolved in 5.0 mL of ethanol / water (85:15, v / v). After stirring at 1000rpm for 1 hour, the above mixed solution was adjusted to pH 5.7 with 1mol / L HCl, and was added dropwise to 20.0mL 0.05% Tween 80 solution (adjusted to pH 4.0) under magnetic stirring (1000rpm) for 30min . Then, the remaining ethanol was removed by rotary evaporator at 37 °C.
[0124] For the method of coating zein-HNK nanoparticles with PSA, the resulting zein-HNK nanoparticle dispersion (5.0 mL) was injected into 5.0 mL of 0.1% PSA (PSA molecular weight 80 kDa) (adjusted to pH 4.0) in the solution, keep stirring (1000rpm) for 1 hour. Freshly prepared samples were centrifuged at 12000 rpm to remove unencapsulated HNK. The resulting sample is HNK-loaded zein / PSA core-shell nanoparticles and is called PSA-zein-HNK (abbreviated as...
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