Bionic drug-loading nano-system combining cell membrane antagonism with nano-enzyme, preparation method and application

A drug-loaded nanotechnology, cell membrane technology, applied in nanomedicine, nanotechnology, nanotechnology and other directions, can solve problems such as disease recurrence, achieve the solution of metastasis, improve the ability to avoid immune clearance and biocompatibility, and improve biodistribution in vivo. Effect

Pending Publication Date: 2021-11-05
SOUTHEAST UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

After chemotherapy in leukemia patients, the leukemia cells in the bone marrow cavity are n

Method used

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  • Bionic drug-loading nano-system combining cell membrane antagonism with nano-enzyme, preparation method and application
  • Bionic drug-loading nano-system combining cell membrane antagonism with nano-enzyme, preparation method and application
  • Bionic drug-loading nano-system combining cell membrane antagonism with nano-enzyme, preparation method and application

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Embodiment 1

[0041] The drug-loaded biomimetic nanozyme system coated with the cell membrane of this embodiment and its preparation method and application include the following steps:

[0042] (1) Preparation of PFOB@PLGA nanospheres

[0043] Dissolve 50 mg of polylactic-glycolic acid (PLGA) and 2 mg of perfluorooctyl bromide (PFOB) in 2 mL of dichloromethane solution ultrasonically, and add to 10 mL of 30% polyvinyl alcohol (PVA) aqueous solution, ice bath and ultrasonically break (The time is 2s, the ultrasound interval is 3s, the power is 120W, and the number of ultrasounds is 80). Then transfer to a 50mL three-necked bottle, and mechanically stir at 400rpm for 4h to volatilize dichloromethane. 8000rpm was centrifuged and washed 3 times, and the precipitate was collected to obtain PFOB-loaded PLGA nanospheres (PFOB@PLGA). Finally, deionized water centrifugal ultrafiltration (ultrafiltration tube molecular weight 30KDa, centrifugation speed 4500rpm, centrifugation time 5min, centrifuga...

Embodiment 2

[0053] Take a small amount of PFOB@PLGA, PFOB@PLGA@Pt and PFOB@PLGA@Pt@CM nanospheres prepared by the above steps, and coat them on the copper grid, and observe the morphology and particle size of the nanospheres with a transmission electron microscope. Such as figure 1 As shown, it can be observed that the PFOB@PLGA nano-microspheres are spherical, the particle size distribution is uniform (60-120nm), there is no agglomeration phenomenon, and the stability is good. The results of EDS energy spectrum show that it mainly contains C, N, O, F, Br Elements, containing the expected basic elements; black Pt nanoparticle clusters can be clearly seen from the PFOB@PLGA@Pt nanosphere diagram, and the EDS energy spectrum results show that it mainly contains C, N, O, S, F, Br , Pt element; the cell membrane can be clearly seen from the PFOB@PLGA@Pt@CM nanospheres, which further confirms that the cell membrane has successfully coated the PFOB@PLGA@Pt nanospheres.

[0054] Such as figur...

Embodiment 3

[0056] Ultrasonic imaging of PLGA@SO, PFOB@PLGA, PFOB@PLGA@Pt nanospheres:

[0057] Inject 0.2mg / mL PLGA@SO, PFOB@PLGA, and PFOB@PLGA@Pt nanosphere aqueous solutions into the gel film respectively, and collect ultrasound images for 0, 5, and 10 minutes respectively. The amount of oxygen is related to the brightness of the ultrasound images .

[0058] Such as Figure 4 As shown, when PLGA nanosphere-loaded soybean oil (PLGA@SO) was used as the control group, the ultrasonic image brightness of PFOB@PLGA nanospheres was stronger, and the ultrasonic image of PFOB@PLGA@Pt nanospheres was the strongest, at least The ultrasonic imaging effect was maintained for 10 minutes, indicating that the PFOB@PLGA@Pt nanospheres have a strong oxygen-carrying function.

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Abstract

The invention discloses a bionic drug-loading nano-system combining cell membrane antagonism with nano-enzyme, a preparation method and application. The system comprises a shell of a cell membrane for loading chemotherapeutic drugs and an inner core of a nano-enzyme modified high-molecular polymer nano-microsphere for loading oxygen-carrying bodies. The bionic nano-carrier maintains the biological activity of cell membrane protein, improves the immune clearance avoiding capacity and biocompatibility of nano-particles, prolongs the blood circulation time of the nano-particles and improves the in-vivo biological distribution of the nano-particles. The drug-loading nano-microsphere is uniform in morphology, has long blood circulation time, can generate a cascade catalytic reaction to generate a large amount of active oxygen, kills leukemia cells, and can effectively inhibit recurrence and metastasis of leukemia; and a new strategy for cooperative treatment of leukemia is provided. The system combines nano-enzyme chemical power treatment with bionic treatment and drug chemotherapy of cell membranes, bone marrow targeted treatment of leukemia can be realized, and the problems of metastasis, recurrence, drug resistance and the like of leukemia are expected to be solved.

Description

technical field [0001] The invention relates to a pharmaceutical preparation and a preparation method thereof, in particular to a biomimetic drug-carrying nano system, a preparation method and an application of a cell membrane antagonism combined with a nanozyme. Background technique [0002] At present, many nanoparticles have been developed as drug delivery systems, and some drug-loaded nanosystems have been approved for the diagnosis and treatment of various cancers. After chemotherapy, the leukemia cells in the bone marrow cavity still remain in the patient's body because the leukemia cells are not completely cleared by the drug, leading to a recurrence of the disease. Stromal cell-derived factor 1 (CXCL12, also known as SDF-1) secreted by stromal cells can activate the specific receptor CXCR4 overexpressed on the surface of leukemia cells, thereby promoting the proliferation, migration and infiltration of leukemia cells to internal organs. The interaction of CXCR4 / CXCL...

Claims

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Application Information

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IPC IPC(8): A61K9/51A61K47/46A61K47/60A61K47/69A61K45/06A61P35/02A61P35/04B82Y5/00B82Y40/00
CPCA61K9/5068A61K47/60A61K47/6931A61K47/6937A61K47/6935A61K45/06A61P35/02A61P35/04B82Y5/00B82Y40/00
Inventor 张宇孔非马明顾宁王建国
Owner SOUTHEAST UNIV
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