Molecule-labeled spathiphyllum (spathiphyllum kochii) directive breeding method

A molecular marker and white palm technology, applied in the field of agricultural biology, can solve the problems of heavy workload, long breeding cycle, difficult to breed new varieties with excellent and stable characters, etc., and achieve the effect of improving directionality and efficiency.

Inactive Publication Date: 2014-07-16
FOSHAN SANSHUI YOUNGPLANTS
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0008] At present, the breeding method of new varieties of white crane taro (white palm) is mainly to select hybrid offspring through artificial cultivation after cross breeding. It is not easy to breed new varieties with excellent and stable traits

Method used

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  • Molecule-labeled spathiphyllum (spathiphyllum kochii) directive breeding method
  • Molecule-labeled spathiphyllum (spathiphyllum kochii) directive breeding method

Examples

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Effect test

Embodiment 1

[0030] Example 1: Molecular marker phenylalanine lyase (PAL) gene and dihydroflavonol 4-reductase (DFR) gene are used for directional selection of the fine color system varieties of Craneus chinensis (Philopsis chinensis)

[0031] (1) Selection and detection of molecular markers: selection of key enzymes in the anthocyanin biosynthesis pathway, phenylalanine lyase (PAL) gene and dihydroflavonol, which are closely related to the color traits of white crane lily (Ph. The conserved region of the 4-reductase (DFR) gene was used as a molecular marker, and a pair of PCR primers were designed according to the above region, and detected by PCR method.

[0032] According to the bioinformatics analysis results of the conserved region of the phenylalanine lyase (PAL) gene, a pair of PCR detection primers (5'-TGTCGACCAGCGTCAACGG-3', 5'-TACTCTTCGTCGTCGTCGCTGA-3') were designed, and the expected amplified region The length is about 1kb or so.

[0033] According to the bioinformatics analys...

Embodiment 2

[0050] Example 2: Molecular marker R2R3-MYB protein gene, bHLH protein gene of myc family and WD40 protein gene for directional breeding of fine color system varieties of Heliconia chinensis (Ph.

[0051] (1) Selection and detection of molecular markers: three regulatory transcription factors in the anthocyanin biosynthesis pathway closely related to the color traits of white crane lily (Ph. The conserved regions of the gene and the WD40 protein gene were used as molecular markers, and a pair of PCR primers were designed based on the above regions, and detected by PCR.

[0052] According to the bioinformatics analysis results of the conserved region of the R2R3-MYB protein gene, a pair of PCR detection primers (5'-CAGGTGGCTAGTCATGCTCA-3', 5'-AGATTCGGATTGTGGTGGAG-3') were designed, and the expected length of the amplified region is about 1.1kb about.

[0053] According to the bioinformatics analysis results of the conserved region of the bHLH protein gene of the myc family, a ...

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Abstract

The present invention discloses a molecule-labeled spathiphyllum (spathiphyllum kochii) directive breeding method, wherein molecule labeling is introduced during a spathiphyllum (spathiphyllum kochii) cross breeding process, and is adopted to label gene or gene cluster related to excellent characteristics of spathiphyllum (spathiphyllum kochii), particularly gene or gene cluster closely related to color phenotype. According to the method, the conventional cross breeding and the molecule labeling technology are organically combined, and the tissue culture seedling production process is integrated, such that the technical problems of long breeding period, high workload, high randomness, and difficult breeding of the new variety with excellent and stable characteristics of the spathiphyllum (spathiphyllum kochii) cross breeding can be solved, the spathiphyllum (spathiphyllum kochii) breeding can have the directionality so as to substantially increase directionality and efficiency of the breeding, and the bred spathiphyllum (spathiphyllum kochii) variety can be used for large-scale tissue culture seedling.

Description

technical field [0001] The invention relates to a method for selecting and breeding white crane lily (white palm), in particular to using molecular markers to carry out directional selection of fine traits of white crane lily (white palm), and belongs to the field of agricultural biotechnology. Background technique [0002] White crane lily (White palm) is native to the tropical rainforest of South America, and is now one of the most popular indoor foliage plants in Europe and the Middle East, and is cultivated all over the world. The plant height is 40-60 cm, with short rhizomes, mostly clustered. Leaves oblong or nearly lanceolate, both ends acuminate, base cuneate. The flower is buddha, slightly fragrant, leaf-shaped, that is, the flower has no petals, but is composed of a white bract and a yellow-white spike, which resembles a palm, so it is called white palm; the flower is large and prominent, and the pedicel is long and thick. Higher than the leaf surface, white or g...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A01H1/02
CPCC12Q1/6895A01H1/02
Inventor 蒋雄辉詹启成周英彪王奎
Owner FOSHAN SANSHUI YOUNGPLANTS
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