Spathiphyllum tetraploid inducing method

A technology of tetraploid and white crane, which is applied in the field of induction of tetraploid of white crane, can solve the problem of not inducing polyploid of white crane, and achieve the effects of easy proliferation and differentiation, good repeatability, and shortened preparation time

Inactive Publication Date: 2017-03-15
三明市农业科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no complete and efficient research report on the induction of polyploidy

Method used

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  • Spathiphyllum tetraploid inducing method
  • Spathiphyllum tetraploid inducing method
  • Spathiphyllum tetraploid inducing method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] step one:

[0078] Select the diploid white crane taro variety "Xiangxiang Baizhang" that grows vigorously, has emerald green leaves, plant height of 2-2.5cm, and is not excessively long and not jointed.

[0079] Step two:

[0080] The stem tip buds of robust tissue cultured plantlets were cut, inoculated in a solid induction medium with a pH value of 5.6 to 5.8, and cultivated for 32 days to obtain callus with buds of tissue cultured plantlets;

[0081] Among them, the formula of solid induction medium is: MS basic medium, 6-benzylaminoadenine (6-BA) 1.0 mg / L, 2,4-D 0.3mg / L, sugar 30g / L, agar 5g / L .

[0082] Step three:

[0083] The callus was inoculated in a solid differentiation medium with a pH value of 5.6 to 5.8, pre-cultured for 5 days, then inoculated in a liquid differentiation medium with a pH value of 5.6 to 5.8 and immersed for 8 days, and in the liquid differentiation medium Shake well every 2 days during the culture period;

[0084] Among them, the fo...

Embodiment 2

[0105] step one:

[0106] Select the diploid white crane taro variety "Meijiu", which grows vigorously, has emerald green leaves, and has a plant height of 1.8-2.2 cm. It is not excessively long and does not joint.

[0107] Step two:

[0108] The stem tip buds of robust tissue cultured plantlets were cut, inoculated in a solid induction medium with a pH value of 5.6 to 5.8, and cultivated for 36 days to obtain callus of tissue cultured plantlets with buds;

[0109] Among them, the formula of the solid induction medium is: MS basic medium, 6-benzylaminoadenine (6-BA) 1.1mg / L, 2,4-D 0.4mg / L, sugar 30g / L, agar 5g / L .

[0110] Step three:

[0111] The callus was inoculated in a solid differentiation medium with a pH value of 5.6 to 5.8, pre-cultured for 6 days, then inoculated in a liquid differentiation medium with a pH value of 5.6 to 5.8 and immersed for 9 days, and in the liquid differentiation medium Shake well every 2 days during the culture period;

[0112] Among them,...

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Abstract

Disclosed is a spathiphyllum tetraploid inducing method. According to the method, the shoot tips and buds of healthy diploid tissue-cultured seedlings are taken as explants and induced into calluses with buds. By means of the healthy diploid tissue-cultured seedlings, a large number of inducing materials can be obtain stably, preparation time of the inducing materials can be shortened, contamination can be reduced. Meanwhile, serving as the explants to be induced into the calluses with buds, the healthy diploid tissue-cultured seedlings has the advantages of being strong in bud activity, easy to proliferate and differentiate, good in repeatability and the like.

Description

technical field [0001] The invention relates to a method for inducing the white crane lily, in particular to a method for inducing the tetraploid of the white crane lily. Background technique [0002] Spathiphyllum floribundum, also known as Spathiphyllum floribundum, is a perennial herbaceous plant belonging to the genus Spathiphyllum of the family Araceae, native to the tropical regions of America and Asia. The leaves of the plant are emerald green, the flower looks like a crane with its head raised, and its color is white and flawless. It is regarded as "the flower of innocence". It is an important potted plant for viewing flowers and leaves, with great ornamental value and economic benefits. [0003] At present, the selection and breeding of new varieties of white crane taro rely on traditional cross-breeding, which has limitations such as lack of a single provenance, low natural variation rate, low natural pollination rate, and inconsistent flowering periods between mal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/08A01H4/00
CPCA01H1/08A01H4/001
Inventor 林辉锋周辉明陈昌铭尚伟林发壮邓才生夏朝水江秋萍姚凤琴王雪凤陈世平魏柳萍
Owner 三明市农业科学研究院
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