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A kind of proliferation preservation method and culture medium of dendrobium officinale somatic embryo

A technology for proliferating culture medium and somatic embryos, which is applied to the proliferation and preservation method of Dendrobium candidum somatic embryos and the field of culture medium, can solve the problems of unfavorable restoration and proliferation of Dendrobium candidum, no research report on somatic embryos of Dendrobium candidum, etc. Disinfection treatment, easy operation, simple effect of cultivation procedure

Inactive Publication Date: 2016-03-09
SHANGHAI ACAD OF AGRI SCI
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Problems solved by technology

Therefore, this method is only suitable for the preservation of Dendrobium officinale, and is not conducive to the restoration of proliferation after preservation of Dendrobium officinale
[0009] So far, there is no research report on the proliferation and preservation of somatic embryos in Dendrobium officinale

Method used

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  • A kind of proliferation preservation method and culture medium of dendrobium officinale somatic embryo
  • A kind of proliferation preservation method and culture medium of dendrobium officinale somatic embryo
  • A kind of proliferation preservation method and culture medium of dendrobium officinale somatic embryo

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Embodiment 1

[0058] 1) Disinfection of explants: get half ripe Dendrobium officinale capsule ( figure 1 ), on an ultra-clean workbench, after sterilizing with 75% alcohol for 45 seconds, soak in 15-20% (volume ratio) sodium hypochlorite aqueous solution for 16 minutes, and rinse with sterile water 3-5 times;

[0059] 2) Induced culture of protocorms: aseptically blot the moisture on the surface of Dendrobium candidum capsules after disinfection, cut open the capsules with a scalpel, pick the seeds, and inoculate them on the protocorm induction medium for weak light cultivation. About 500 lux, after 25 days, transfer to the light for cultivation. The light intensity is 2000 lux. After continuing to cultivate for 20-25 days, the explants germinate and form conical protocorms ( figure 2 ), the protocorm induction rate reaches 95%;

[0060] Protocorm induction medium formula is: MS basic medium, 6-BA1.0mg / L, NAA0.5mg / L, CH1.0g / L, AC0.5g / L, sucrose 3% + agar powder 6-7g / L ;

[0061] 3) Indu...

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Abstract

The invention discloses a method and a culture medium for proliferation storage of somatic embryos of dendrobiumcandidum. The method comprises the following steps: (1) disinfecting explants; (2) performing induction culture on protocorm; (3) inducing the somatic embryos; (4) performing proliferation storage culture on the somatic embryos; (5) performing recovery growth and proliferation culture on the somatic embryos; (6) germinating the somatic embryos; and (7) performing rooting culture on strong test-tube plantlets. According to the method for the proliferation storage culture of the somatic embryos of the dendrobiumcandidum, the culture can be continuously stored for 12 months and can be proliferated by 40 times without subculture during storage, the operation is easy, the cost is low, the recovery culture reaction is quick, and a large quantity of the somatic embryos can be proliferated or the somatic embryos can be stored under the control of the proliferation speed within a relatively short time according to market and production needs.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method and culture medium for proliferation and preservation of dendrobium candidum somatic embryos. Background technique [0002] Dendrobium candidum (Dendrobium candidum), commonly known as Tiepi Fengdou, is a perennial herb of the genus Dendrobium orchidaceae. It is a precious traditional Chinese medicine with important medicinal value. development value. In recent years, due to people's excessive excavation of it, its wild resources have decreased sharply, and it has become increasingly endangered. It has been listed as a national key protected plant. Because Dendrobium candidum has high requirements on the ecological environment, the reproductive rate of Dendrobium candidum is low under natural conditions and the growth rate is slow. The efficiency of breeding Dendrobium candidum by traditional methods is very low. Therefore, the research on tiss...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 殷丽青孙翊张永春周音朱建军杨柳燕李水根范晓芬
Owner SHANGHAI ACAD OF AGRI SCI
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