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Method for keeping genetic diversity of Jian carps

A technology of genetic diversity and genetic distance, applied in the field of fish breeding, can solve the problems of inability to understand the genetic relationship of different individuals, germplasm degradation, easy inbreeding, etc., to improve genetic diversity, slow germplasm degradation, and avoid close relatives. effects of mating

Inactive Publication Date: 2017-03-08
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI +1
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AI Technical Summary

Problems solved by technology

[0004] Traditional Jian carp breeding methods can only select parents based on phenotypes such as growth, body shape, and physical fitness, and cannot understand the genetic relationship between different individuals, which is prone to inbreeding, resulting in germplasm degradation

Method used

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  • Method for keeping genetic diversity of Jian carps
  • Method for keeping genetic diversity of Jian carps
  • Method for keeping genetic diversity of Jian carps

Examples

Experimental program
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Embodiment 1

[0025] Step 1, individual marking: select 900 healthy individuals (450 males and 450 females) from the Jian carp breeding colony as parent fish, put PIT electronic markers into the abdominal cavity of each fish, and measure body length, Body height, body thickness.

[0026] Step 2. Establishing a template: 0.5 mL of blood was collected from the tail vein of each fish, and 30 μL of blood cells were taken to extract genomic DNA.

[0027] Step 3, Genotyping: Screen out 15 microsatellite loci with 4 to 13 alleles for analysis of genetic distance and genetic diversity. The primer sequences of microsatellite loci are shown in Table 1 The specific method is to use the 15 microsatellite loci and take the genomic DNA as a template to carry out PCR amplification. The total volume of the PCR reaction is 10 μL, which contains 1.0 μL of 10× reaction buffer, Mg 2+ 2mmol / L, dNTP 200μmol / L, upstream and downstream primers 0.2μmol / L, Taq enzyme 0.3U, DNA 50ng~80ng, make up to 10μL with steri...

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Abstract

The invention discloses a method for keeping the genetic diversity of Jian carps. The method comprises the steps of individual tagging, template creating, genetic typing, family building, breeding file establishing and parent updating. Compared with the prior art, inbreeding of the Jian carps can be effectively avoided, the genetic diversity of Jian carp groups is kept and increased, and genetical characterization decline of the Jian carps is slowed down; in addition, breeding is assisted through molecular markers, a breeding file is established, genetic distances of the groups are analyzed by combining all parameters in the file, and therefore dominant groups are selectively reserved, and descendants excellent in genetic characterization are obtained through mating.

Description

technical field [0001] The invention belongs to the field of fish breeding, and relates to a method for assisted breeding by using molecular markers, in particular to a breeding method for avoiding inbreeding of carps and maintaining genetic diversity. Background technique [0002] Jian carp (Cyprinus carplo vat.jian) has the advantages of fast growth, good body shape, blue-gray color, good meat quality and taste, high feed conversion rate, strong adaptability and stress resistance, etc. It is bred through family selection, multi-line hybridization and female A carp variety with stable genetic traits successfully bred by the comprehensive breeding technology combined with nuclear development. Genetic diversity is the core of biodiversity. In general, the richer the genetic diversity of a species, the stronger its adaptability and survival ability to the environment. species degeneration. [0003] Although Jian carp has relatively comprehensive and excellent traits, due to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68A01K61/10
CPCA01K61/00C12Q1/6888C12Q2600/124C12Q2600/156Y02A40/81
Inventor 李建林俞菊华傅纯洁李红霞唐永凯于凡
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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