Anther culture and detoxifying method of fragrant lily

A technology of lilies and lilies, applied in the field of horticulture, can solve problems such as low cultivation efficiency and failure to meet production requirements

Inactive Publication Date: 2016-10-12
王纪芝
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The main reason is that the existing lily anther culture efficienc

Method used

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  • Anther culture and detoxifying method of fragrant lily

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] In July 2012, the anthers of the Perfume Lily cultivars White Fairy and Marshal were selected for flower cultivation to induce detoxification. The detailed steps are as follows:

[0058] (1) Preparation of Perfume Lily Anther Induction Medium

[0059] Formulated per liter of distilled water from:

[0060] Macroelements: KNO 3 2600mg / L, NH 4 NO 3 1700mg / L, KH 2 PO 4 460mg / L, MgSO 4 ∙7H 2 O 370mg / L, CaCl 2 ∙2H 2 O 840mg / L;

[0061] Trace elements: MnSO 4 ∙4H 2 O 22.5mg / L, ZnSO 4 ∙7H 2 O 8.5mg / L, H 3 BO 3 6.25mg / L, KI 0.825mg / L, CuSO 4 ∙5H 2 O 0.025mg / L, CoCl∙6H 2 O 0.025mg / L, NaMoO 4 ∙2H 2 O 0.25mg / L;

[0062] Iron salt: Na 2 -EDTA∙2H 2 O 37.5mg / L, FeSO 4 ∙7H 2 O 27.5mg / L;

[0063] Organic Ingredients: Inositol 100mg / L, Vitamin B1 0.1mg / L, Vitamin B6 0.5mg / L, Niacin 0.5mg / L, Glycine 2.0mg / L, Serine 0.8mg / L;

[0064] Hormone: 2,4-D 3.4mg / L, KT 2.2mg / L;

[0065] Coagulant: vegetable gel 6.25g / L;

[0066] Active additives: coconut milk 15g / L,...

Embodiment 2

[0082] In April 2013, two conventional detoxification methods for lily lilies, namely, shoot apex meristem culture combined with heat treatment and shoot apex culture combined with ribavirin chemical treatment, were used to detoxify the lily cultivars Baijingling and Marshal. With Marshal virus-free seedlings, the white spirit and marshal virus-free seedlings frozen strains obtained by adopting the technical route of the present invention in Example 1 were used to detect 3 main viruses infecting lily: cucumber mosaic virus (CMV), lily asymptomatic Virus (LSV) and Lily Mottle Virus (LMoV), compare the difference in the detoxification effect of the technical route of the present invention and the conventional detoxification method on common lily viruses. The identification method adopts multiple RT-PCR technology (refer to "RT-PCR detection and detoxification technology research of three main viruses of lily" by Chen Jin et al.). 1.

[0083]

[0084] It can be found from Tab...

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Abstract

The invention provides a detoxification method for cultivating anthers of Perfume Lily. The detailed steps of the detoxification method include: (1) preparing an anther induction medium for Perfume Lily; (2) selecting materials for inoculating anthers; (3) disinfecting and inoculating anthers; (4) Anther induction culture; (5) Embryogenic callus differentiation and subculture; (6) Hardening and transplanting of lily bulbs; (7) Elimination of haploid plants of lily. The anther culture and detoxification method of perfume lily of the present invention obtains perfume lily pollen plants through the cultivation of perfume lily anthers, and then removes the haploid plants doped in the population to obtain perfume lily diploid plants, and further detects the lily virus and finds that the The detoxification method almost achieves 100% detoxification, greatly improves the lily germplasm, and obtains detoxified lily plants with greatly improved yield and quality.

Description

technical field [0001] The invention relates to the field of horticulture, in particular to a method for cultivating and detoxifying anthers of lilies of perfume through an anther cultivating approach for efficiently removing lily viruses. Background technique [0002] lily( Lilium spp.) is the Liliaceae ( Liliaceae ) Lilium ( Lilium ) is a perennial bulbous flower, which is loved by people for its large flowers, rich colors, beautiful flower shapes, and pure meaning. It is one of the important cut flower varieties in the world today. Westerners use lily as a symbol of holiness. About 1,000 years ago, the temple capital of King Solomon of Israel was decorated with lily. The flower name of the lily is to commemorate the Virgin Mary, who has been regarded by Christianity as a symbol of purity since ancient times, so its flower language is purity. At present, there are more than 100 kinds of lilies in the world, and there are more than 60 kinds of lilies in my country. L...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王纪芝宋晓燕
Owner 王纪芝
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