Artificial breeding method for loaches
A technology of artificial breeding and loach, applied in fish farming, application, climate change adaptation, etc., can solve problems such as premature transshipment of loach seedlings, affecting the survival rate of loach larvae, and vicious cycle of germplasm resources, etc., to improve The survival rate of larvae and juveniles, the effect of ensuring good hatching water quality and reducing the cost of breeding
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example 1
[0029] A. Selection and screening of parent loach: select with the naked eye the parent loach with a winter age of 2 years, which is correct in body shape, strong in physique, free from disease and injury, normal in body color, and has a lot of mucus on the body surface, and passes through the stainless steel with a sieve hole of 1.5 cm. The parent loach is screened by the round hole screen, and the smaller individuals are removed and put into the holding pond;
[0030] B. Artificial induction of labor: Put the anesthetic (Ms-222, ethyl m-aminobenzoate methanesulfonate, commonly known as Yu Ding) into the holding pond, the water temperature is 24°C, the dose is 40mg / L, and after the pro-loach is anesthetized, use it again. Oxytocic agent (HCG: chorionic gonadotropin, DOM: dioconone maleate, LRH-A: luteinizing hormone releasing hormone, 0.7% sodium chloride solution of normal saline) carries out injection oxytocin to parent loach;
[0031] C, debonding: the fertilized eggs obta...
example 2
[0036] Select winter age to be the pro-loach of 3 years, the sieve hole diameter of sieve is 1.5cm, temporarily raise pond water temperature 25 ℃, anesthesia consumption is 80mg / L, debonding is carried out under 24 ℃ of water temperature, all the other are with example 1.
example 3
[0038] Select the pro-loach that winter age is 4 years, the sieve hole diameter of sieve is 2cm, temporarily raise pond water temperature 24 ℃, anesthesia consumption is 100mg / L, debonding is carried out under 25 ℃ of water temperature, all the other are with example 1.
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