Artificial breeding method for loaches
A technology of artificial breeding and loach, applied in fish farming, application, climate change adaptation, etc., can solve problems such as premature transshipment of loach seedlings, affecting the survival rate of loach larvae, and vicious cycle of germplasm resources, etc., to improve The survival rate of larvae and juveniles, the effect of ensuring good hatching water quality and reducing the cost of breeding
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[0028] Example 1
[0029] A. Selection and sieving of pro-loach: Use the naked eye to select the pro-loach that is 2 years old in winter with a correct body shape, a strong physique, no disease, no injury, normal body color, and a lot of mucus on the body surface, and pass through the stainless steel with a mesh of 1.5 cm The round hole screen screens the parent loach, removes the smaller individuals and puts them in the holding tank;
[0030] B. Artificial induction of labor: Put the anesthetic (Ms-222, ethyl m-aminobenzoate methanesulfonate, commonly known as Yu Diane) into the holding tank, the water temperature is 24 ℃, the dosage is 40 mg / L, and then use it again after the pro-loach is anesthetized Oxytocin (HCG: chorionic gonadotropin, DOM: dioxone maleate, LRH-A: luteinizing hormone releasing hormone, 0.7% sodium chloride solution in physiological saline) is injected to induce labor to the mother;
[0031] C. Debonding: Put the fertilized egg obtained in step B into the debon...
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[0035] Example 2
[0036] Choosing a three-year winter loach with a mesh diameter of 1.5 cm, a holding tank water temperature of 25°C, an anesthetic dosage of 80 mg / L, and debonding at a water temperature of 24°C. The rest is the same as in Example 1.
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[0037] Example 3
[0038] Choose a four-year winter loach, the mesh diameter of the screen is 2cm, the water temperature of the holding tank is 24°C, the dosage of anesthetic is 100mg / L, and the debonding is performed at the water temperature of 25°C. The rest is the same as in Example 1.
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