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Rapid propagation method adopting tissue culture for Croton tiglium

A tissue culture and croton technology, applied in the field of plant cultivation, can solve the problems of low natural survival rate and the like

Inactive Publication Date: 2016-11-09
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, croton is mainly propagated by seeds, and the natural survival rate is low, which cannot meet the demand of the market

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Sieve with water to remove empty croton seeds, soak in saturated washing powder for 30 minutes, rinse with running water, soak in sterile water for 2 days, fully imbibition, replace with 1% KI and soak for 15 hours the night before inoculation, and soak in 75% ethanol before inoculation 1 min, 0.1% mercuric chloride soaking and disinfecting for 25 min, washing with sterile water 5 times, draining for later use, putting the sterilized croton seeds into VW medium for induction of sterile seedlings, adding 45 g / L sucrose and 7 g / L carrageenan L, pH5.8, light 2000lx, temperature 25°C, cut off the roots of the induced sterile seedlings and insert them into the medium 3 / 4VW+NAA0.2mg / L+Ad0.25mg / L for bud proliferation culture, add sucrose 30g / L , carrageenan 7g / L, pH 5.8, light 2000lx, temperature 25°C, the sprouts after proliferation were refrigerated at 4°C for 10 days, inserted into medium 3 / 4VW+NAA0.2mg / L for rooting induction, and added sucrose 50g / L, carrageenan 4.5g / L, ...

Embodiment 2

[0011] Sieve with water to remove empty croton seeds, soak in saturated washing powder for 30 minutes, rinse with running water, soak in sterile water for 2 days, fully imbibition, replace with 1% KI and soak for 15 hours the night before inoculation, and soak in 75% ethanol before inoculation 1 min, 0.1% mercuric chloride soaking and disinfecting for 25 min, washing with sterile water 5 times, draining for later use, putting the sterilized croton seeds into VW medium for induction of sterile seedlings, adding 45 g / L sucrose and 7 g / L carrageenan L, pH5.8, light 2000lx, temperature 25°C, cut off the roots of the induced sterile seedlings and insert them into the medium 3 / 4VW+NAA0.2mg / L+Ad0.25mg / L for bud proliferation culture, add sucrose 30g / L, carrageenan 7g / L, pH5.8, light 2000lx, temperature 25°C, the sprouts after proliferation were refrigerated at 4°C for 10 days, inserted into medium 3 / 4VW+NAA0.2mg / L for rooting induction, additional Sucrose 50g / L, carrageenan 4.5g / L, ...

Embodiment 3

[0013] Sieve with water to remove empty croton seeds, soak in saturated washing powder for 30 minutes, rinse with running water, soak in sterile water for 2 days, fully imbibition, replace with 1% KI and soak for 15 hours the night before inoculation, and soak in 75% ethanol before inoculation 1 min, 0.1% mercuric chloride soaking and disinfecting for 25 min, washing with sterile water 5 times, draining for later use, putting the sterilized croton seeds into VW medium for induction of sterile seedlings, adding 45 g / L sucrose and 7 g / L carrageenan L, pH5.8, light 2000lx, temperature 25°C, cut off the roots of the induced sterile seedlings and insert them into the medium 3 / 4VW+NAA0.2mg / L+Ad0.25mg / L for bud proliferation culture, add sucrose 30g / L, carrageenan 7g / L, pH5.8, light 2000lx, temperature 25°C, the sprouts after proliferation were refrigerated at 4°C for 10 days, inserted into medium 3 / 4VW+NAA0.2mg / L for rooting induction, additional Sucrose 50g / L, carrageenan 4.5g / L, ...

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PUM

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Abstract

The invention discloses a rapid propagation method adopting tissue culture for Croton tiglium. The rapid propagation method comprises steps of disinfection of Croton tiglium seeds, acquisition of aseptic seedlings; proliferation of cluster buds, rooting culture, strong seedling culture, acclimatization and transplanting. According to the method, a Croton tiglium regeneration system is established and can be applied to storage and reproduction of germplasm resources of the Croton tiglium as well as research on genetic improvement, and defects of a traditional mode for improving Croton tiglium germplasm can be overcome.

Description

technical field [0001] The invention relates to a rapid propagation method for tissue culture of croton, belonging to the technical field of plant cultivation. Background technique [0002] Croton, Euphorbiaceae, also known as Shuangyanlong, Jiangzi, Mengzishu, etc. Croton tiglium L. , shrub or small tree, 3-6 meters high, shoots are sparsely stellate pilose, branches glabrous, leaves papery, ovate, sparsely elliptic, raceme, terminal, capsule elliptic, sparsely Short stellate hairs or nearly glabrous, oval seeds, about 1 cm long, 6-7 mm in diameter, flowering period from April to June, born in villages or mountain forests, prefers warm and humid climate, not cold-resistant, afraid of frost It is suitable for cultivation in areas with a temperature of 17-19°C, an annual rainfall of 1000mm, a year-round sunshine of 1000h, and a frost-free period of more than 300d. When the temperature is lower than 3°C, all the leaves of the seedlings will die. , Sandy loam soil with good d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 杨成东
Owner NANJING ZELANG AGRI DEV
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