Breeding method of specific pathogen free reed vole

A specific pathogen-free, cultivation method technology, applied in the field of cultivation of specific pathogen-free oriental voles, to achieve the effect of large purification and high-efficiency filtration

Inactive Publication Date: 2010-08-18
上海西普尔-必凯实验动物有限公司
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Problems solved by technology

However, the drug purification method has the following defects: when the obtained animals are tested for bacteria and parasites, some bacteria, viruses and parasites that should not exist in rodent clean animals can be found

Method used

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  • Breeding method of specific pathogen free reed vole
  • Breeding method of specific pathogen free reed vole

Examples

Experimental program
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Embodiment Construction

[0022] The donor mouse vole orientalis used in this example is an ordinary closed group of Dongting Lake population raised in open facilities; the suckling mice SPF grade ICR mice and SD rats are raised in PR series rats and rats isolators.

[0023] The fetal mouse vole orientalis, suckling mice SPF grade ICR mice and SD rats were all provided by Shanghai Xipuer-Bikay Experimental Animal Co., Ltd.

[0024] The cultivation method of SPF level oriental vole of the present invention, carries out successively according to the following steps:

[0025] (1) Pregnancy Three vole orientalis, male and female, were selected respectively, and the male and female pairs were raised in three cages, and each cage was raised with one vole orientalis male and one male respectively, so that the female mice were pregnant respectively;

[0026] (2) Determining the laparotomy time of the pregnant rat. Under non-anesthesia, touch both sides of the abdomen of the pregnant rat with fingers. When the ...

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Abstract

The invention relates to a breeding method of a specific pathogen free (SPF for short) reed vole, which comprises the following steps in sequence: (1) breeding a male reed vole and a female reed vole as a pair in each cage and making the female reed vole pregnant; (2) determining the laparotomy time of the pregnant reed vole through palpation and observation of the pregnant reed vole; (3) disinfecting to prepare for laparotomy and extraction; (4) carrying out laparotomy and extraction for the reed vole; (5) breeding the newly born reed vole with a SPF CR mice within 1-5 day(s) and breeding the newly born reed vole with a SPF SD rat within 6-15 days; and (6) carrying out microbiologic and parasite detection for items necessary to be detected in GB14922.1-2001 and GB14922.2-2001. The laparotomy and extraction are carried out under sterile conditions, and two types of breeding mouse completely different from the carcass supply mice are adopted for breeding and purification, thereby achieving the purification effect with more advantages than the medical purification method, successfully obtaining the SPF reed vole for the first time, and laying a foundation for the establishment of an ideal animal model for schistosomiasis resistance, non-alcoholic fatty liver and other diseases and the research on pathogenic mechanisms of the diseases.

Description

technical field [0001] The invention relates to a method for cultivating specific pathogen-free (abbreviated as SPF) grade experimental animals in the field of pharmaceutical products and biotechnology, in particular to a method for cultivating SPF grade oriental voles. Background technique [0002] Oriental vole belongs to Cricetidae, Microtinae, and Microtus. It is a common rodent with a wide distribution in my country, covering 18 provinces and cities from Heilongjiang in the north to Guangdong in the south. ,autonomous region. [0003] As early as the 1950s, people noticed that oriental voles had natural resistance to schistosomiasis. In recent years, researchers have studied the genetics, biology, and anti-schistosomiasis mechanism of oriental voles raised and bred in ordinary environments. A more systematic observation and research has been carried out in multiple directions, and it has been found that in addition to its anti-schistosome infection properties, it is als...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61D1/08
Inventor 柏熊陈国强杨玉琴王晓东谢建云邢正弘高诚
Owner 上海西普尔-必凯实验动物有限公司
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