Breeding method of specific pathogen free reed vole
A specific pathogen-free, cultivation method technology, applied in the field of cultivation of specific pathogen-free oriental voles, to achieve the effect of large purification and high-efficiency filtration
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[0022] The donor mouse vole orientalis used in this example is an ordinary closed group of Dongting Lake population raised in open facilities; the suckling mice SPF grade ICR mice and SD rats are raised in PR series rats and rats isolators.
[0023] The fetal mouse vole orientalis, suckling mice SPF grade ICR mice and SD rats were all provided by Shanghai Xipuer-Bikay Experimental Animal Co., Ltd.
[0024] The cultivation method of SPF level oriental vole of the present invention, carries out successively according to the following steps:
[0025] (1) Pregnancy Three vole orientalis, male and female, were selected respectively, and the male and female pairs were raised in three cages, and each cage was raised with one vole orientalis male and one male respectively, so that the female mice were pregnant respectively;
[0026] (2) Determining the laparotomy time of the pregnant rat. Under non-anesthesia, touch both sides of the abdomen of the pregnant rat with fingers. When the ...
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